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Interaction of silver nanoparticles with an environmentally beneficial bacterium, Pseudomonas chlororaphis.
J Hazard Mater. 2011 Apr 15; 188(1-3):428-35.JH

Abstract

This study explores the potential antimicrobial mechanisms of commercial silver nanoparticles (Ag NPs) in the environmental bacterium, Pseudomonas chlororaphis O6. The 10nm size NPs aggregated in water, as demonstrated by atomic force microscopy. Solubility of the NPs at 10mg/L was 0.28 mg/L (pH 6) and 2.3mg/L (pH 7); release from 10mg/L bulk Ag was below detection. The NPs eliminated cell culturability at 3mg/L, whereas no effect was observed at 10mg/L bulk Ag. Zeta potential measurements revealed that the NPs were negatively charged; unlike Ag ions, their addition to the negatively charged cells did not change cell charge at pH 6, but showed a trend to reduce cell charge at pH 7. Isolated extracellular polymeric substances (EPS) from PcO6 was polydisperse, with negative charge that was neutralized by Ag ions, but not by the NPs. Addition of EPS eliminated Ag NP's toxicity in cells lacking EPS. Intracellular accumulation of OH was not detected in NP-treated cells; however, the use of scavengers suggested the NPs caused extracellular H(2)O(2) production. No evidence was found for loss of membrane integrity upon treatment with the NPs. Our findings indicate that growth of environmental bacteria could be impaired by Ag NPs, depending on the extent of EPS production.

Authors+Show Affiliations

Department of Biological Engineering, Utah State University, Logan, UT 84322, USA. cdimkpa@usu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21339046

Citation

Dimkpa, Christian O., et al. "Interaction of Silver Nanoparticles With an Environmentally Beneficial Bacterium, Pseudomonas Chlororaphis." Journal of Hazardous Materials, vol. 188, no. 1-3, 2011, pp. 428-35.
Dimkpa CO, Calder A, Gajjar P, et al. Interaction of silver nanoparticles with an environmentally beneficial bacterium, Pseudomonas chlororaphis. J Hazard Mater. 2011;188(1-3):428-35.
Dimkpa, C. O., Calder, A., Gajjar, P., Merugu, S., Huang, W., Britt, D. W., McLean, J. E., Johnson, W. P., & Anderson, A. J. (2011). Interaction of silver nanoparticles with an environmentally beneficial bacterium, Pseudomonas chlororaphis. Journal of Hazardous Materials, 188(1-3), 428-35. https://doi.org/10.1016/j.jhazmat.2011.01.118
Dimkpa CO, et al. Interaction of Silver Nanoparticles With an Environmentally Beneficial Bacterium, Pseudomonas Chlororaphis. J Hazard Mater. 2011 Apr 15;188(1-3):428-35. PubMed PMID: 21339046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction of silver nanoparticles with an environmentally beneficial bacterium, Pseudomonas chlororaphis. AU - Dimkpa,Christian O, AU - Calder,Alyssa, AU - Gajjar,Priyanka, AU - Merugu,Srinivas, AU - Huang,Wenjie, AU - Britt,David W, AU - McLean,Joan E, AU - Johnson,William P, AU - Anderson,Anne J, Y1 - 2011/02/20/ PY - 2010/10/20/received PY - 2011/01/27/revised PY - 2011/01/31/accepted PY - 2011/2/23/entrez PY - 2011/2/23/pubmed PY - 2011/7/16/medline SP - 428 EP - 35 JF - Journal of hazardous materials JO - J Hazard Mater VL - 188 IS - 1-3 N2 - This study explores the potential antimicrobial mechanisms of commercial silver nanoparticles (Ag NPs) in the environmental bacterium, Pseudomonas chlororaphis O6. The 10nm size NPs aggregated in water, as demonstrated by atomic force microscopy. Solubility of the NPs at 10mg/L was 0.28 mg/L (pH 6) and 2.3mg/L (pH 7); release from 10mg/L bulk Ag was below detection. The NPs eliminated cell culturability at 3mg/L, whereas no effect was observed at 10mg/L bulk Ag. Zeta potential measurements revealed that the NPs were negatively charged; unlike Ag ions, their addition to the negatively charged cells did not change cell charge at pH 6, but showed a trend to reduce cell charge at pH 7. Isolated extracellular polymeric substances (EPS) from PcO6 was polydisperse, with negative charge that was neutralized by Ag ions, but not by the NPs. Addition of EPS eliminated Ag NP's toxicity in cells lacking EPS. Intracellular accumulation of OH was not detected in NP-treated cells; however, the use of scavengers suggested the NPs caused extracellular H(2)O(2) production. No evidence was found for loss of membrane integrity upon treatment with the NPs. Our findings indicate that growth of environmental bacteria could be impaired by Ag NPs, depending on the extent of EPS production. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/21339046/Interaction_of_silver_nanoparticles_with_an_environmentally_beneficial_bacterium_Pseudomonas_chlororaphis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(11)00167-1 DB - PRIME DP - Unbound Medicine ER -