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Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.).
Theor Appl Genet. 2011 May; 122(8):1503-16.TA

Abstract

Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In the present study, using conventional polymerase chain reaction (PCR) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying LMW-GS gene family members. Based on sequence alignment of 13 LMW-GS genes previously identified in the Chinese bread wheat variety Xiaoyan 54 and other genes available in GenBank, PCR primers were developed and assigned to conserved sequences spanning the length polymorphism regions of LMW-GS genes. After PCR amplification, 17 DNA fragments in Xiaoyan 54 were detected using capillary electrophoresis. In total, 13 fragments were identical to previously identified LMW-GS genes, and the other 4 were derived from unique LMW-GS genes by sequencing. This marker system was also used to identify LMW-GS genes in Chinese Spring and its group 1 nulli-tetrasomic lines. Among the 17 detected DNA fragments, 4 were located on chromosome 1A, 5 on 1B, and 8 on 1D. The results suggest that this marker system is useful for large-scale identification of LMW-GS genes in bread wheat varieties, and for the selection of desirable LMW-GS genes to improve the bread-making quality in wheat molecular breeding programmes.

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Authors+Show Affiliations

Zhang X
State Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing, China.
Liu D
No affiliation info available
Yang W
No affiliation info available
Liu K
No affiliation info available
Sun J
No affiliation info available
Guo X
No affiliation info available
Li Y
No affiliation info available
Wang D
No affiliation info available
Ling H
No affiliation info available
Zhang A
No affiliation info available

MeSH

Base SequenceDNA PrimersElectrophoresis, CapillaryGenetic MarkersGlutensMolecular Sequence DataMultigene FamilyPolymerase Chain ReactionSequence Analysis, DNATriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21344181

Citation

Zhang, Xiaofei, et al. "Development of a New Marker System for Identifying the Complex Members of the Low-molecular-weight Glutenin Subunit Gene Family in Bread Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 122, no. 8, 2011, pp. 1503-16.
Zhang X, Liu D, Yang W, et al. Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2011;122(8):1503-16.
Zhang, X., Liu, D., Yang, W., Liu, K., Sun, J., Guo, X., Li, Y., Wang, D., Ling, H., & Zhang, A. (2011). Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 122(8), 1503-16. https://doi.org/10.1007/s00122-011-1550-7
Zhang X, et al. Development of a New Marker System for Identifying the Complex Members of the Low-molecular-weight Glutenin Subunit Gene Family in Bread Wheat (Triticum Aestivum L.). Theor Appl Genet. 2011;122(8):1503-16. PubMed PMID: 21344181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). AU - Zhang,Xiaofei, AU - Liu,Dongcheng, AU - Yang,Wenlong, AU - Liu,Kunfan, AU - Sun,Jiazhu, AU - Guo,Xiaoli, AU - Li,Yiwen, AU - Wang,Daowen, AU - Ling,Hongqing, AU - Zhang,Aimin, Y1 - 2011/02/23/ PY - 2010/08/04/received PY - 2011/02/05/accepted PY - 2011/2/24/entrez PY - 2011/2/24/pubmed PY - 2011/8/23/medline SP - 1503 EP - 16 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 122 IS - 8 N2 - Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In the present study, using conventional polymerase chain reaction (PCR) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying LMW-GS gene family members. Based on sequence alignment of 13 LMW-GS genes previously identified in the Chinese bread wheat variety Xiaoyan 54 and other genes available in GenBank, PCR primers were developed and assigned to conserved sequences spanning the length polymorphism regions of LMW-GS genes. After PCR amplification, 17 DNA fragments in Xiaoyan 54 were detected using capillary electrophoresis. In total, 13 fragments were identical to previously identified LMW-GS genes, and the other 4 were derived from unique LMW-GS genes by sequencing. This marker system was also used to identify LMW-GS genes in Chinese Spring and its group 1 nulli-tetrasomic lines. Among the 17 detected DNA fragments, 4 were located on chromosome 1A, 5 on 1B, and 8 on 1D. The results suggest that this marker system is useful for large-scale identification of LMW-GS genes in bread wheat varieties, and for the selection of desirable LMW-GS genes to improve the bread-making quality in wheat molecular breeding programmes. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/21344181/Development_of_a_new_marker_system_for_identifying_the_complex_members_of_the_low_molecular_weight_glutenin_subunit_gene_family_in_bread_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-011-1550-7 DB - PRIME DP - Unbound Medicine ER -