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Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings.
Biol Trace Elem Res. 2011 Dec; 143(3):1758-76.BT

Abstract

In order to observe the possible regulatory role of selenium (Se) in relation to the changes in ascorbate (AsA) glutathione (GSH) levels and to the activities of antioxidant and glyoxalase pathway enzymes, rapeseed (Brassica napus) seedlings were grown in Petri dishes. A set of 10-day-old seedlings was pretreated with 25 μM Se (Sodium selenate) for 48 h. Two levels of drought stress (10% and 20% PEG) were imposed separately as well as on Se-pretreated seedlings, which were grown for another 48 h. Drought stress, at any level, caused a significant increase in GSH and glutathione disulfide (GSSG) content; however, the AsA content increased only under mild stress. The activity of ascorbate peroxidase (APX) was not affected by drought stress. The monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) activity increased only under mild stress (10% PEG). The activity of dehydroascorbate reductase (DHAR), glutathione S-transferase (GST), glutathione peroxidase (GPX), and glyoxalase I (Gly I) activity significantly increased under any level of drought stress, while catalase (CAT) and glyoxalase II (Gly II) activity decreased. A sharp increase in hydrogen peroxide (H(2)O(2)) and lipid peroxidation (MDA content) was induced by drought stress. On the other hand, Se-pretreated seedlings exposed to drought stress showed a rise in AsA and GSH content, maintained a high GSH/GSSG ratio, and evidenced increased activities of APX, DHAR, MDHAR, GR, GST, GPX, CAT, Gly I, and Gly II as compared with the drought-stressed plants without Se. These seedlings showed a concomitant decrease in GSSG content, H(2)O(2), and the level of lipid peroxidation. The results indicate that the exogenous application of Se increased the tolerance of the plants to drought-induced oxidative damage by enhancing their antioxidant defense and methylglyoxal detoxification systems.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hasanuzzaman M
Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan. mhzsauag@yahoo.com
Fujita M
No affiliation info available

MeSH

Adaptation, PhysiologicalAntioxidantsAscorbic AcidBrassicaDroughtsGlutathioneLipid PeroxidationPyruvaldehydeSeleniumStress, PhysiologicalUp-Regulation

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21347652

Citation

Hasanuzzaman, Mirza, and Masayuki Fujita. "Selenium Pretreatment Upregulates the Antioxidant Defense and Methylglyoxal Detoxification System and Confers Enhanced Tolerance to Drought Stress in Rapeseed Seedlings." Biological Trace Element Research, vol. 143, no. 3, 2011, pp. 1758-76.
Hasanuzzaman M, Fujita M. Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings. Biol Trace Elem Res. 2011;143(3):1758-76.
Hasanuzzaman, M., & Fujita, M. (2011). Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings. Biological Trace Element Research, 143(3), 1758-76. https://doi.org/10.1007/s12011-011-8998-9
Hasanuzzaman M, Fujita M. Selenium Pretreatment Upregulates the Antioxidant Defense and Methylglyoxal Detoxification System and Confers Enhanced Tolerance to Drought Stress in Rapeseed Seedlings. Biol Trace Elem Res. 2011;143(3):1758-76. PubMed PMID: 21347652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium pretreatment upregulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings. AU - Hasanuzzaman,Mirza, AU - Fujita,Masayuki, Y1 - 2011/02/24/ PY - 2011/01/12/received PY - 2011/02/08/accepted PY - 2011/2/25/entrez PY - 2011/2/25/pubmed PY - 2012/3/17/medline SP - 1758 EP - 76 JF - Biological trace element research JO - Biol Trace Elem Res VL - 143 IS - 3 N2 - In order to observe the possible regulatory role of selenium (Se) in relation to the changes in ascorbate (AsA) glutathione (GSH) levels and to the activities of antioxidant and glyoxalase pathway enzymes, rapeseed (Brassica napus) seedlings were grown in Petri dishes. A set of 10-day-old seedlings was pretreated with 25 μM Se (Sodium selenate) for 48 h. Two levels of drought stress (10% and 20% PEG) were imposed separately as well as on Se-pretreated seedlings, which were grown for another 48 h. Drought stress, at any level, caused a significant increase in GSH and glutathione disulfide (GSSG) content; however, the AsA content increased only under mild stress. The activity of ascorbate peroxidase (APX) was not affected by drought stress. The monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) activity increased only under mild stress (10% PEG). The activity of dehydroascorbate reductase (DHAR), glutathione S-transferase (GST), glutathione peroxidase (GPX), and glyoxalase I (Gly I) activity significantly increased under any level of drought stress, while catalase (CAT) and glyoxalase II (Gly II) activity decreased. A sharp increase in hydrogen peroxide (H(2)O(2)) and lipid peroxidation (MDA content) was induced by drought stress. On the other hand, Se-pretreated seedlings exposed to drought stress showed a rise in AsA and GSH content, maintained a high GSH/GSSG ratio, and evidenced increased activities of APX, DHAR, MDHAR, GR, GST, GPX, CAT, Gly I, and Gly II as compared with the drought-stressed plants without Se. These seedlings showed a concomitant decrease in GSSG content, H(2)O(2), and the level of lipid peroxidation. The results indicate that the exogenous application of Se increased the tolerance of the plants to drought-induced oxidative damage by enhancing their antioxidant defense and methylglyoxal detoxification systems. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/21347652/Selenium_pretreatment_upregulates_the_antioxidant_defense_and_methylglyoxal_detoxification_system_and_confers_enhanced_tolerance_to_drought_stress_in_rapeseed_seedlings_ L2 - https://dx.doi.org/10.1007/s12011-011-8998-9 DB - PRIME DP - Unbound Medicine ER -