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[UPLC fingerprint for quality assessment of ginsenosides of ginseng radix et rhizoma].
Yao Xue Xue Bao. 2010 Oct; 45(10):1296-300.YX

Abstract

This paper is aimed to establish the method of fingerprint analysis of chemical constituents by reversed-phase ultra-performance liquid chromatography (UPLC) for the quality control of the roots and rhizomes of Panax ginseng (Ginseng Radix et Rhizoma). The method was performed on a ACQUITY UPLC BEH C18 (50 mm x 2.1 mm ID, 1.7 microm) with a mixed mobile phase of water and acetonitrile in a gradient mode. The flow rate was 0.3 mL x min(-1) and the wavelength of measurement was 203 nm. Eleven batches of the Ginseng Radix et Rhizoma were determined. The UPLC chromatographic fingerprints of chemical constituents were established from the eleven batches of the Ginseng Radix et Rhizoma and showed fifteen characteristic common peaks, among which fifteen peaks were recognized and nine compounds (ginsenosides Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3 and Rd) were determined by comparison with chromatographic behaviors and UV spectra of the authentic compounds. The eleven batches of samples were classified as two clusters by hierarchical clustering analysis (HCA) and principle component analysis (PCA), and six samples were confirmed to establish the mutual model. The quality was assessed by similarity evaluation system for chromatographic fingerprint of TCM (2004B Version). The convenient and high specific method can be used to identify and evaluate the quality of the Ginseng Radix et Rhizoma.

Authors+Show Affiliations

Zhang CY
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Dong L
No affiliation info available
Chen SL
No affiliation info available
Xie CX
No affiliation info available
Chang DL
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCluster AnalysisGinsenosidesPanaxPlant RootsPlants, MedicinalPrincipal Component AnalysisQuality ControlRhizome

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21348309

Citation

Zhang, Cui-ying, et al. "[UPLC Fingerprint for Quality Assessment of Ginsenosides of Ginseng Radix Et Rhizoma]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 45, no. 10, 2010, pp. 1296-300.
Zhang CY, Dong L, Chen SL, et al. [UPLC fingerprint for quality assessment of ginsenosides of ginseng radix et rhizoma]. Yao Xue Xue Bao. 2010;45(10):1296-300.
Zhang, C. Y., Dong, L., Chen, S. L., Xie, C. X., & Chang, D. L. (2010). [UPLC fingerprint for quality assessment of ginsenosides of ginseng radix et rhizoma]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 45(10), 1296-300.
Zhang CY, et al. [UPLC Fingerprint for Quality Assessment of Ginsenosides of Ginseng Radix Et Rhizoma]. Yao Xue Xue Bao. 2010;45(10):1296-300. PubMed PMID: 21348309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [UPLC fingerprint for quality assessment of ginsenosides of ginseng radix et rhizoma]. AU - Zhang,Cui-ying, AU - Dong,Liang, AU - Chen,Shi-lin, AU - Xie,Cai-xiang, AU - Chang,Duan-ling, PY - 2011/2/26/entrez PY - 2011/2/26/pubmed PY - 2012/9/20/medline SP - 1296 EP - 300 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 45 IS - 10 N2 - This paper is aimed to establish the method of fingerprint analysis of chemical constituents by reversed-phase ultra-performance liquid chromatography (UPLC) for the quality control of the roots and rhizomes of Panax ginseng (Ginseng Radix et Rhizoma). The method was performed on a ACQUITY UPLC BEH C18 (50 mm x 2.1 mm ID, 1.7 microm) with a mixed mobile phase of water and acetonitrile in a gradient mode. The flow rate was 0.3 mL x min(-1) and the wavelength of measurement was 203 nm. Eleven batches of the Ginseng Radix et Rhizoma were determined. The UPLC chromatographic fingerprints of chemical constituents were established from the eleven batches of the Ginseng Radix et Rhizoma and showed fifteen characteristic common peaks, among which fifteen peaks were recognized and nine compounds (ginsenosides Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3 and Rd) were determined by comparison with chromatographic behaviors and UV spectra of the authentic compounds. The eleven batches of samples were classified as two clusters by hierarchical clustering analysis (HCA) and principle component analysis (PCA), and six samples were confirmed to establish the mutual model. The quality was assessed by similarity evaluation system for chromatographic fingerprint of TCM (2004B Version). The convenient and high specific method can be used to identify and evaluate the quality of the Ginseng Radix et Rhizoma. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/21348309/[UPLC_fingerprint_for_quality_assessment_of_ginsenosides_of_ginseng_radix_et_rhizoma]_ L2 - https://antibodies.cancer.gov/detail/CPTC-RB1-1 DB - PRIME DP - Unbound Medicine ER -