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Toxicokinetics of the ciguatoxin P-CTX-1 in rats after intraperitoneal or oral administration.
Toxicology. 2011 Jun 18; 284(1-3):1-6.T

Abstract

Ciguatoxins are voltage-gated selective algal toxins responsible for ciguatera fish poisoning. In this study we evaluate the toxicokinetics of one of the most common ciguatoxins found in the Pacific, the P-CTX-1, in rat after an oral or intraperitoneal (ip) dose of 0.26 μg/kg body weight. We report levels of ciguatoxin activity assessed over time in blood, urine and feces, and at 4 days in liver, muscle and brain, using the functional in vitro N2A cytotoxicity assay. Following exposure, the ciguatoxin activity exhibited a rapid systemic absorption that was followed by a bi-exponential decline, and data best fit a two-compartment model analysis. Maximum blood concentrations were reached at 1.97 and 0.43 h after the oral and ip dose, respectively. Ciguatoxin elimination from blood was slow with terminal half lives (t(½)β) estimated at 82 h for oral and 112 h for ip dosing. Ciguatoxin activity remained in liver, muscle and brain 96 h after ip and oral administration. While smaller amounts appeared in the urine, the main excretion route was feces, with peak rates reaching > 10 pg P-CTX-1 equivalents/h in both routes of administration. Assay guided fractionation showed the presence in the feces and liver of peaks of activity corresponding to the P-CTX-1 and to other less polar metabolites. In conclusion, biologically active ciguatoxins are detectable in blood, liver, muscle and brain, and continued to be excreted in urine and feces 4 days following exposure. Blood, as well as urine and feces may be useful matrices for low-invasive testing methods for ciguatera clinical cases.

Authors+Show Affiliations

Marine Biotoxins Program, Center for Coastal Environmental Health and Biomolecular Research, NOAA-National Ocean Service, 219 Fort Johnson Road, Charleston, SC 29412, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

21349314

Citation

Bottein, Marie-Yasmine Dechraoui, et al. "Toxicokinetics of the Ciguatoxin P-CTX-1 in Rats After Intraperitoneal or Oral Administration." Toxicology, vol. 284, no. 1-3, 2011, pp. 1-6.
Bottein MY, Wang Z, Ramsdell JS. Toxicokinetics of the ciguatoxin P-CTX-1 in rats after intraperitoneal or oral administration. Toxicology. 2011;284(1-3):1-6.
Bottein, M. Y., Wang, Z., & Ramsdell, J. S. (2011). Toxicokinetics of the ciguatoxin P-CTX-1 in rats after intraperitoneal or oral administration. Toxicology, 284(1-3), 1-6. https://doi.org/10.1016/j.tox.2011.02.005
Bottein MY, Wang Z, Ramsdell JS. Toxicokinetics of the Ciguatoxin P-CTX-1 in Rats After Intraperitoneal or Oral Administration. Toxicology. 2011 Jun 18;284(1-3):1-6. PubMed PMID: 21349314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicokinetics of the ciguatoxin P-CTX-1 in rats after intraperitoneal or oral administration. AU - Bottein,Marie-Yasmine Dechraoui, AU - Wang,Zhihong, AU - Ramsdell,John S, Y1 - 2011/02/22/ PY - 2010/12/17/received PY - 2011/02/08/revised PY - 2011/02/15/accepted PY - 2011/2/26/entrez PY - 2011/2/26/pubmed PY - 2011/6/29/medline SP - 1 EP - 6 JF - Toxicology JO - Toxicology VL - 284 IS - 1-3 N2 - Ciguatoxins are voltage-gated selective algal toxins responsible for ciguatera fish poisoning. In this study we evaluate the toxicokinetics of one of the most common ciguatoxins found in the Pacific, the P-CTX-1, in rat after an oral or intraperitoneal (ip) dose of 0.26 μg/kg body weight. We report levels of ciguatoxin activity assessed over time in blood, urine and feces, and at 4 days in liver, muscle and brain, using the functional in vitro N2A cytotoxicity assay. Following exposure, the ciguatoxin activity exhibited a rapid systemic absorption that was followed by a bi-exponential decline, and data best fit a two-compartment model analysis. Maximum blood concentrations were reached at 1.97 and 0.43 h after the oral and ip dose, respectively. Ciguatoxin elimination from blood was slow with terminal half lives (t(½)β) estimated at 82 h for oral and 112 h for ip dosing. Ciguatoxin activity remained in liver, muscle and brain 96 h after ip and oral administration. While smaller amounts appeared in the urine, the main excretion route was feces, with peak rates reaching > 10 pg P-CTX-1 equivalents/h in both routes of administration. Assay guided fractionation showed the presence in the feces and liver of peaks of activity corresponding to the P-CTX-1 and to other less polar metabolites. In conclusion, biologically active ciguatoxins are detectable in blood, liver, muscle and brain, and continued to be excreted in urine and feces 4 days following exposure. Blood, as well as urine and feces may be useful matrices for low-invasive testing methods for ciguatera clinical cases. SN - 1879-3185 UR - https://www.unboundmedicine.com/medline/citation/21349314/Toxicokinetics_of_the_ciguatoxin_P_CTX_1_in_rats_after_intraperitoneal_or_oral_administration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(11)00073-4 DB - PRIME DP - Unbound Medicine ER -