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Protection against 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells.
J Ethnopharmacol. 2011 Apr 26; 135(1):34-42.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for screening therapeutic agents for degenerative disorders such as Parkinson's disease.

MATERIALS AND METHODS

SH-SY5Y human neuroblastoma cells were used to analyze the protective effects of water extract of ginseng (WEG) against multiple parameters such as MPP(+)-induced viability, oxidative injury, expression of Bax, Bcl-2, cytochrome c and cleaved caspase-3.

RESULTS

WEG exerted inhibitory effect on cell death, overproduction of ROS, elevated Bax/Bcl-2 ratio, release of cytochrome c and activation of caspase-3 expression in MPP(+)-treated SH-SY5Y cells.

CONCLUSIONS

WEG exhibited significant protective effects against MPP(+)-induced cytotoxicity in SH-SY5Y cells possibly through the suppression of ROS generation and the inhibition of mitochondria-dependent apoptotic pathway.

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Authors+Show Affiliations

Hu S
Department of Applied Biology and Chemical Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, Hong Kong, China.
Han R
No affiliation info available
Mak S
No affiliation info available
Han Y
No affiliation info available

MeSH

1-Methyl-4-phenylpyridiniumAntioxidantsApoptosisCaspase 3Cell Line, TumorCytochromes cDrug Evaluation, PreclinicalEnzyme InhibitorsHerbicidesHumansMitochondriaNeuroblastomaNeurotoxinsPanaxParkinson DiseasePhytotherapyPlant ExtractsProto-Oncogene Proteins c-bcl-2Reactive Oxygen Speciesbcl-2-Associated X Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21349320

Citation

Hu, Shengquan, et al. "Protection Against 1-methyl-4-phenylpyridinium Ion (MPP+)-induced Apoptosis By Water Extract of Ginseng (Panax Ginseng C.A. Meyer) in SH-SY5Y Cells." Journal of Ethnopharmacology, vol. 135, no. 1, 2011, pp. 34-42.
Hu S, Han R, Mak S, et al. Protection against 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells. J Ethnopharmacol. 2011;135(1):34-42.
Hu, S., Han, R., Mak, S., & Han, Y. (2011). Protection against 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells. Journal of Ethnopharmacology, 135(1), 34-42. https://doi.org/10.1016/j.jep.2011.02.017
Hu S, et al. Protection Against 1-methyl-4-phenylpyridinium Ion (MPP+)-induced Apoptosis By Water Extract of Ginseng (Panax Ginseng C.A. Meyer) in SH-SY5Y Cells. J Ethnopharmacol. 2011 Apr 26;135(1):34-42. PubMed PMID: 21349320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection against 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells. AU - Hu,Shengquan, AU - Han,Renwen, AU - Mak,Shinghung, AU - Han,Yifan, Y1 - 2011/02/22/ PY - 2010/07/05/received PY - 2011/02/14/revised PY - 2011/02/15/accepted PY - 2011/2/26/entrez PY - 2011/2/26/pubmed PY - 2011/12/13/medline SP - 34 EP - 42 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 135 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for screening therapeutic agents for degenerative disorders such as Parkinson's disease. MATERIALS AND METHODS: SH-SY5Y human neuroblastoma cells were used to analyze the protective effects of water extract of ginseng (WEG) against multiple parameters such as MPP(+)-induced viability, oxidative injury, expression of Bax, Bcl-2, cytochrome c and cleaved caspase-3. RESULTS: WEG exerted inhibitory effect on cell death, overproduction of ROS, elevated Bax/Bcl-2 ratio, release of cytochrome c and activation of caspase-3 expression in MPP(+)-treated SH-SY5Y cells. CONCLUSIONS: WEG exhibited significant protective effects against MPP(+)-induced cytotoxicity in SH-SY5Y cells possibly through the suppression of ROS generation and the inhibition of mitochondria-dependent apoptotic pathway. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21349320/Protection_against_1_methyl_4_phenylpyridinium_ion__MPP+__induced_apoptosis_by_water_extract_of_ginseng__Panax_ginseng_C_A__Meyer__in_SH_SY5Y_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00102-4 DB - PRIME DP - Unbound Medicine ER -