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The role of automated measurement of RBC subpopulations in differential diagnosis of microcytic anemia and β-thalassemia screening.
Am J Clin Pathol 2011; 135(3):374-9AJ

Abstract

Cell counter-based formulas have been used in the differential diagnosis of microcytic anemia. The measurement of RBC subpopulations is now available on the Sysmex XE 5000 analyzer (Sysmex, Kobe, Japan). We describe the new formulas: % microcytic - % hypochromic; and % microcytic - % hypochromic - red cell distribution width (RDW), derived from the percentages of microcytic and hypochromic RBCs. The present study aimed to prospectively evaluate the reliability of these new formulas in the differential diagnosis of microcytosis and β-thalassemia screening compared with already published indices. The indices were calculated for a set of 250 iron-deficient patients and 270 β-thalassemia carriers. Independent samples t test and receiver-operating characteristics analysis were applied. The % microcytic - % hypochromic - RDW, % microcytic - % hypochromic, and Green and King indices provided higher areas under the curve. The % microcytic - % hypochromic - RDW was the most reliable index evaluated, with 100% sensitivity and 92.6% specificity. This index can be used to efficiently screen patients with microcytosis for further hematologic studies to confirm β-thalassemia.

Authors+Show Affiliations

Hematology Laboratory, Hospital Galdakao-Usansolo, Galdakao, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21350090

Citation

Urrechaga, Eloísa, et al. "The Role of Automated Measurement of RBC Subpopulations in Differential Diagnosis of Microcytic Anemia and Β-thalassemia Screening." American Journal of Clinical Pathology, vol. 135, no. 3, 2011, pp. 374-9.
Urrechaga E, Borque L, Escanero JF. The role of automated measurement of RBC subpopulations in differential diagnosis of microcytic anemia and β-thalassemia screening. Am J Clin Pathol. 2011;135(3):374-9.
Urrechaga, E., Borque, L., & Escanero, J. F. (2011). The role of automated measurement of RBC subpopulations in differential diagnosis of microcytic anemia and β-thalassemia screening. American Journal of Clinical Pathology, 135(3), pp. 374-9. doi:10.1309/AJCPJRH1I0XTNFGA.
Urrechaga E, Borque L, Escanero JF. The Role of Automated Measurement of RBC Subpopulations in Differential Diagnosis of Microcytic Anemia and Β-thalassemia Screening. Am J Clin Pathol. 2011;135(3):374-9. PubMed PMID: 21350090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of automated measurement of RBC subpopulations in differential diagnosis of microcytic anemia and β-thalassemia screening. AU - Urrechaga,Eloísa, AU - Borque,Luís, AU - Escanero,Jesús F, PY - 2011/2/26/entrez PY - 2011/2/26/pubmed PY - 2011/4/13/medline SP - 374 EP - 9 JF - American journal of clinical pathology JO - Am. J. Clin. Pathol. VL - 135 IS - 3 N2 - Cell counter-based formulas have been used in the differential diagnosis of microcytic anemia. The measurement of RBC subpopulations is now available on the Sysmex XE 5000 analyzer (Sysmex, Kobe, Japan). We describe the new formulas: % microcytic - % hypochromic; and % microcytic - % hypochromic - red cell distribution width (RDW), derived from the percentages of microcytic and hypochromic RBCs. The present study aimed to prospectively evaluate the reliability of these new formulas in the differential diagnosis of microcytosis and β-thalassemia screening compared with already published indices. The indices were calculated for a set of 250 iron-deficient patients and 270 β-thalassemia carriers. Independent samples t test and receiver-operating characteristics analysis were applied. The % microcytic - % hypochromic - RDW, % microcytic - % hypochromic, and Green and King indices provided higher areas under the curve. The % microcytic - % hypochromic - RDW was the most reliable index evaluated, with 100% sensitivity and 92.6% specificity. This index can be used to efficiently screen patients with microcytosis for further hematologic studies to confirm β-thalassemia. SN - 1943-7722 UR - https://www.unboundmedicine.com/medline/citation/21350090/The_role_of_automated_measurement_of_RBC_subpopulations_in_differential_diagnosis_of_microcytic_anemia_and_β_thalassemia_screening_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/AJCPJRH1I0XTNFGA DB - PRIME DP - Unbound Medicine ER -