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Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis.
Cytokine. 2011 May; 54(2):109-16.C

Abstract

The potential mechanisms for altered matrix metalloproteinase (MMP) or tissue inhibitors of matrix metalloproteinase (TIMP) function in patients with syphilis and HIV-1 co-infection (HIV-S) was unclear. To determine the expression of MMP-2, 9 and TIMP-1, 2, 4 in the serum and cerebrospinal fluid (CSF) of HIV-S patients, a total of 20 HIV-S patients and 8 controls were enrolled in a HIV-1 clinical cohort for diagnosis of neurosyphilis in Taiwan. Serum and CSF concentrations of MMP-2, 9, and TIMP-1, 2, 4 were determined by ELISA. Gelatin zymography was used to detect the expression of MMP-2 and MMP-9 in the CSF. Neurosyphilis was defined as a CSF white blood cell count ≥ 20 cells/μL or a reactive CSF Venereal Disease Research Laboratory (VDRL). All the patients with HIV-S were males. Most (85%) had sex with men (MSM) and serum rapid plasma reagin (RPR) titers of ≥ 1:32. The median age was 35 years (IQR 30-43). The median CD4 T cell counts at the time of the diagnosis of syphilis were 270 cells/μL (IQR 96-484). Ten patients (50%) had neurosyphilis based on a reactive CSF VDRL test (n=8) or increased CSF white cell counts ≥ 20/μL (n=2). The concentrations of CSF MMP-9, TIMP-1, and TIMP-2 were significantly higher in patients with HIV-S than the controls (P<0.05). The CSF TIMP-4 concentrations were significantly lower in those with HIV-S (452 pg/ml) than controls (3101 pg/ml), P<0001. There were no significant differences in serum concentrations between the groups. The only finding that distinguished HIV-1 patients with from those without neurosyphilis is a significant higher expression of CSF MMP-9. In conclusion, the MMP/TIMP system was found to be dysregulated in patients with HIV-S regardless of whether they met the laboratory definition of neurosyphilis. The CSF level of MMP-9 was the only measure that distinguished those with or without neurosyphilis.

Authors+Show Affiliations

Department of Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21354815

Citation

Tsai, Hung-Chin, et al. "Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in the Serum and Cerebrospinal Fluid of Patients With HIV-1 Infection and Syphilis or Neurosyphilis." Cytokine, vol. 54, no. 2, 2011, pp. 109-16.
Tsai HC, Ye SY, Kunin CM, et al. Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis. Cytokine. 2011;54(2):109-16.
Tsai, H. C., Ye, S. Y., Kunin, C. M., Lee, S. S., Wann, S. R., Tai, M. H., Shi, M. H., Liu, Y. C., & Chen, Y. S. (2011). Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis. Cytokine, 54(2), 109-16. https://doi.org/10.1016/j.cyto.2011.02.007
Tsai HC, et al. Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in the Serum and Cerebrospinal Fluid of Patients With HIV-1 Infection and Syphilis or Neurosyphilis. Cytokine. 2011;54(2):109-16. PubMed PMID: 21354815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis. AU - Tsai,Hung-Chin, AU - Ye,Shin-Yu, AU - Kunin,Calvin M, AU - Lee,Susan Shin-Jung, AU - Wann,Shue-Ren, AU - Tai,Ming-Hong, AU - Shi,Min-Hong, AU - Liu,Yung-Ching, AU - Chen,Yao-Shen, Y1 - 2011/02/26/ PY - 2010/01/10/received PY - 2011/01/18/revised PY - 2011/02/03/accepted PY - 2011/3/1/entrez PY - 2011/3/1/pubmed PY - 2011/7/30/medline SP - 109 EP - 16 JF - Cytokine JO - Cytokine VL - 54 IS - 2 N2 - The potential mechanisms for altered matrix metalloproteinase (MMP) or tissue inhibitors of matrix metalloproteinase (TIMP) function in patients with syphilis and HIV-1 co-infection (HIV-S) was unclear. To determine the expression of MMP-2, 9 and TIMP-1, 2, 4 in the serum and cerebrospinal fluid (CSF) of HIV-S patients, a total of 20 HIV-S patients and 8 controls were enrolled in a HIV-1 clinical cohort for diagnosis of neurosyphilis in Taiwan. Serum and CSF concentrations of MMP-2, 9, and TIMP-1, 2, 4 were determined by ELISA. Gelatin zymography was used to detect the expression of MMP-2 and MMP-9 in the CSF. Neurosyphilis was defined as a CSF white blood cell count ≥ 20 cells/μL or a reactive CSF Venereal Disease Research Laboratory (VDRL). All the patients with HIV-S were males. Most (85%) had sex with men (MSM) and serum rapid plasma reagin (RPR) titers of ≥ 1:32. The median age was 35 years (IQR 30-43). The median CD4 T cell counts at the time of the diagnosis of syphilis were 270 cells/μL (IQR 96-484). Ten patients (50%) had neurosyphilis based on a reactive CSF VDRL test (n=8) or increased CSF white cell counts ≥ 20/μL (n=2). The concentrations of CSF MMP-9, TIMP-1, and TIMP-2 were significantly higher in patients with HIV-S than the controls (P<0.05). The CSF TIMP-4 concentrations were significantly lower in those with HIV-S (452 pg/ml) than controls (3101 pg/ml), P<0001. There were no significant differences in serum concentrations between the groups. The only finding that distinguished HIV-1 patients with from those without neurosyphilis is a significant higher expression of CSF MMP-9. In conclusion, the MMP/TIMP system was found to be dysregulated in patients with HIV-S regardless of whether they met the laboratory definition of neurosyphilis. The CSF level of MMP-9 was the only measure that distinguished those with or without neurosyphilis. SN - 1096-0023 UR - https://www.unboundmedicine.com/medline/citation/21354815/Expression_of_matrix_metalloproteinases_and_their_tissue_inhibitors_in_the_serum_and_cerebrospinal_fluid_of_patients_with_HIV_1_infection_and_syphilis_or_neurosyphilis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(11)00036-6 DB - PRIME DP - Unbound Medicine ER -