Tags

Type your tag names separated by a space and hit enter

Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle.
Acta Orthop Traumatol Turc. 2010; 44(6):476-83.AO

Abstract

OBJECTIVES

The aim of the study was to investigate the effect of hyperbaric oxygen-preconditioning (HBO-PC) and ozone-preconditioning (O3-PC) on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion (I/R) in rat skeletal muscle.

METHODS

Thirty-two Wistar-Albino-type male rats included in the study were divided into four groups of equal number: 1) sham operation, 2) I/R, 3) I/R+HBO-PC, or 4) I/R+O3-PC. One session of 3-4 L/min 100% oxygenation for 60 min at 3 absolute atmosphere (ATA) was defined as one dose of HBO; in total, 7 doses of HBO-PC were administered before ischemia. One dose of O3 comprised 0.7 mg/kg ozone/oxygen mixture, administered intraperitoneally; a total of 4 doses of O3-PC were administered before ischemia. The I/R model was performed in anesthetized rats by clipping right femoral artery to induce 2 h ischemia followed by 22 h of reperfusion. The right gastrocnemius muscle and venous blood samples were harvested. Tissue was assayed for levels of malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). Serum was assayed to measure the level of nitrite-nitrate (NOx).

RESULTS

Tissue MDA level, SOD activity, and serum NOx level were increased, whereas GSHPx was decreased in the I/R group. MDA and NOx levels were decreased, whereas GSH-Px activity was increased in both the I/R+HBO-PC and I/R+O3-PC groups. SOD activity was increased in the I/R+O3-PC group, but did not change significantly in the I/R+HBO-PC group. iNOS staining score and intensity were lower in the I/R+HBO-PC and I/R+O3-PC groups than I/R group.

CONCLUSION

Both O3-PC and HBO-PC reduced tissue lipid peroxidation, NOx levels, and iNOS staining scores in the experimental I/R model. Our data suggest that HBO-PC and O3-PC protect against oxidative/nitrosative stress induced by I/R in rat skeletal muscle.

Authors+Show Affiliations

GATA, Department of Orthopedics, Ankara, Turkey. drkenankoca@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21358255

Citation

Koca, Kenan, et al. "Effect of Hyperbaric Oxygen and Ozone Preconditioning On Oxidative/nitrosative Stress Induced By Tourniquet Ischemia/reperfusion in Rat Skeletal Muscle." Acta Orthopaedica Et Traumatologica Turcica, vol. 44, no. 6, 2010, pp. 476-83.
Koca K, Yurttaş Y, Yıldız C, et al. Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthop Traumatol Turc. 2010;44(6):476-83.
Koca, K., Yurttaş, Y., Yıldız, C., Caycı, T., Uysal, B., & Korkmaz, A. (2010). Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopaedica Et Traumatologica Turcica, 44(6), 476-83. https://doi.org/10.3944/AOTT.2010.2327
Koca K, et al. Effect of Hyperbaric Oxygen and Ozone Preconditioning On Oxidative/nitrosative Stress Induced By Tourniquet Ischemia/reperfusion in Rat Skeletal Muscle. Acta Orthop Traumatol Turc. 2010;44(6):476-83. PubMed PMID: 21358255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. AU - Koca,Kenan, AU - Yurttaş,Yüksel, AU - Yıldız,Cemil, AU - Caycı,Tuncer, AU - Uysal,Bülent, AU - Korkmaz,Ahmet, PY - 2011/3/2/entrez PY - 2011/3/2/pubmed PY - 2011/7/1/medline SP - 476 EP - 83 JF - Acta orthopaedica et traumatologica turcica JO - Acta Orthop Traumatol Turc VL - 44 IS - 6 N2 - OBJECTIVES: The aim of the study was to investigate the effect of hyperbaric oxygen-preconditioning (HBO-PC) and ozone-preconditioning (O3-PC) on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion (I/R) in rat skeletal muscle. METHODS: Thirty-two Wistar-Albino-type male rats included in the study were divided into four groups of equal number: 1) sham operation, 2) I/R, 3) I/R+HBO-PC, or 4) I/R+O3-PC. One session of 3-4 L/min 100% oxygenation for 60 min at 3 absolute atmosphere (ATA) was defined as one dose of HBO; in total, 7 doses of HBO-PC were administered before ischemia. One dose of O3 comprised 0.7 mg/kg ozone/oxygen mixture, administered intraperitoneally; a total of 4 doses of O3-PC were administered before ischemia. The I/R model was performed in anesthetized rats by clipping right femoral artery to induce 2 h ischemia followed by 22 h of reperfusion. The right gastrocnemius muscle and venous blood samples were harvested. Tissue was assayed for levels of malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). Serum was assayed to measure the level of nitrite-nitrate (NOx). RESULTS: Tissue MDA level, SOD activity, and serum NOx level were increased, whereas GSHPx was decreased in the I/R group. MDA and NOx levels were decreased, whereas GSH-Px activity was increased in both the I/R+HBO-PC and I/R+O3-PC groups. SOD activity was increased in the I/R+O3-PC group, but did not change significantly in the I/R+HBO-PC group. iNOS staining score and intensity were lower in the I/R+HBO-PC and I/R+O3-PC groups than I/R group. CONCLUSION: Both O3-PC and HBO-PC reduced tissue lipid peroxidation, NOx levels, and iNOS staining scores in the experimental I/R model. Our data suggest that HBO-PC and O3-PC protect against oxidative/nitrosative stress induced by I/R in rat skeletal muscle. SN - 2589-1294 UR - https://www.unboundmedicine.com/medline/citation/21358255/Effect_of_hyperbaric_oxygen_and_ozone_preconditioning_on_oxidative/nitrosative_stress_induced_by_tourniquet_ischemia/reperfusion_in_rat_skeletal_muscle_ L2 - http://www.aott.org.tr/index.php/aott/article/view/4807/2556 DB - PRIME DP - Unbound Medicine ER -