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Hepatitis E virus ORF3 antigens derived from genotype 1 and 4 viruses are detected with varying efficiencies by an anti-HEV enzyme immunoassay.
J Med Virol. 2011 May; 83(5):827-32.JM

Abstract

The function of the hepatitis E virus (HEV) open reading frame 3 (ORF3) protein product remains unclear but it is able to induce a strong antibody response following HEV infection. Therefore, it has been used in some enzyme immunoassays (EIAs) for detecting anti-HEV antibody. In order to evaluate the difference in antigenicity of HEV ORF3 polypeptides derived from genotypes 1 and 4, two EIAs were developed, based on ORF3 polypeptides from genotypes 1 and 4 HEV. Serial weekly serum samples from two rhesus monkeys vaccinated with ORF3 antigens derived from the genotype 4 ORF3 protein and nine rhesus monkeys experimentally infected with genotypes 1 and 4 HEV were tested for anti-HEV using the assays. HEV ORF3 antigens derived from viruses of genotypes 1 and 4 showed different patterns of reactivity with sera obtained from monkeys immunized with ORF3 antigens or infected experimentally with HEV. The genotype 1 ORF3 polypeptide exhibited stronger reactivity with the sera from monkeys infected with genotype 1 than the genotype 4 ORF3 polypeptide. The genotype 4 ORF3 polypeptide demonstrated stronger reactivity with the sera from monkeys infected with genotype 4 than did the genotype 1 ORF3 polypeptide. The HEV ORF3 polypeptide contains genotype-specific antigens and the antigen-antibody reactions between the same genotypes were stronger than those between different genotypes.

Authors+Show Affiliations

Department of Cell Biology, National Institute for Control of Pharmaceutical and Biological Products, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21360543

Citation

Ma, Hongxia, et al. "Hepatitis E Virus ORF3 Antigens Derived From Genotype 1 and 4 Viruses Are Detected With Varying Efficiencies By an anti-HEV Enzyme Immunoassay." Journal of Medical Virology, vol. 83, no. 5, 2011, pp. 827-32.
Ma H, Song X, Harrison TJ, et al. Hepatitis E virus ORF3 antigens derived from genotype 1 and 4 viruses are detected with varying efficiencies by an anti-HEV enzyme immunoassay. J Med Virol. 2011;83(5):827-32.
Ma, H., Song, X., Harrison, T. J., Zhang, H., Huang, W., & Wang, Y. (2011). Hepatitis E virus ORF3 antigens derived from genotype 1 and 4 viruses are detected with varying efficiencies by an anti-HEV enzyme immunoassay. Journal of Medical Virology, 83(5), 827-32. https://doi.org/10.1002/jmv.22032
Ma H, et al. Hepatitis E Virus ORF3 Antigens Derived From Genotype 1 and 4 Viruses Are Detected With Varying Efficiencies By an anti-HEV Enzyme Immunoassay. J Med Virol. 2011;83(5):827-32. PubMed PMID: 21360543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatitis E virus ORF3 antigens derived from genotype 1 and 4 viruses are detected with varying efficiencies by an anti-HEV enzyme immunoassay. AU - Ma,Hongxia, AU - Song,Xiaoguo, AU - Harrison,Tim J, AU - Zhang,Heqiu, AU - Huang,Weijin, AU - Wang,Youchun, Y1 - 2011/02/25/ PY - 2010/12/09/accepted PY - 2011/3/2/entrez PY - 2011/3/2/pubmed PY - 2011/7/16/medline SP - 827 EP - 32 JF - Journal of medical virology JO - J Med Virol VL - 83 IS - 5 N2 - The function of the hepatitis E virus (HEV) open reading frame 3 (ORF3) protein product remains unclear but it is able to induce a strong antibody response following HEV infection. Therefore, it has been used in some enzyme immunoassays (EIAs) for detecting anti-HEV antibody. In order to evaluate the difference in antigenicity of HEV ORF3 polypeptides derived from genotypes 1 and 4, two EIAs were developed, based on ORF3 polypeptides from genotypes 1 and 4 HEV. Serial weekly serum samples from two rhesus monkeys vaccinated with ORF3 antigens derived from the genotype 4 ORF3 protein and nine rhesus monkeys experimentally infected with genotypes 1 and 4 HEV were tested for anti-HEV using the assays. HEV ORF3 antigens derived from viruses of genotypes 1 and 4 showed different patterns of reactivity with sera obtained from monkeys immunized with ORF3 antigens or infected experimentally with HEV. The genotype 1 ORF3 polypeptide exhibited stronger reactivity with the sera from monkeys infected with genotype 1 than the genotype 4 ORF3 polypeptide. The genotype 4 ORF3 polypeptide demonstrated stronger reactivity with the sera from monkeys infected with genotype 4 than did the genotype 1 ORF3 polypeptide. The HEV ORF3 polypeptide contains genotype-specific antigens and the antigen-antibody reactions between the same genotypes were stronger than those between different genotypes. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/21360543/Hepatitis_E_virus_ORF3_antigens_derived_from_genotype_1_and_4_viruses_are_detected_with_varying_efficiencies_by_an_anti_HEV_enzyme_immunoassay_ L2 - https://doi.org/10.1002/jmv.22032 DB - PRIME DP - Unbound Medicine ER -