Citation
Fu, Ni-Na, et al. "Rapid Analysis of Phosphoamino Acids From Phosvitin By Near-infrared Cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate Potassium Derivatization and Polyacrylamide-coated CE With LIF Detection." Electrophoresis, vol. 32, no. 6-7, 2011, pp. 712-9.
Fu NN, Wang H, Zhang HS. Rapid analysis of phosphoamino acids from phosvitin by near-infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium derivatization and polyacrylamide-coated CE with LIF detection. Electrophoresis. 2011;32(6-7):712-9.
Fu, N. N., Wang, H., & Zhang, H. S. (2011). Rapid analysis of phosphoamino acids from phosvitin by near-infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium derivatization and polyacrylamide-coated CE with LIF detection. Electrophoresis, 32(6-7), 712-9. https://doi.org/10.1002/elps.201000540
Fu NN, Wang H, Zhang HS. Rapid Analysis of Phosphoamino Acids From Phosvitin By Near-infrared Cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate Potassium Derivatization and Polyacrylamide-coated CE With LIF Detection. Electrophoresis. 2011;32(6-7):712-9. PubMed PMID: 21365656.
TY - JOUR
T1 - Rapid analysis of phosphoamino acids from phosvitin by near-infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium derivatization and polyacrylamide-coated CE with LIF detection.
AU - Fu,Ni-Na,
AU - Wang,Hong,
AU - Zhang,Hua-Shan,
Y1 - 2011/03/01/
PY - 2010/10/13/received
PY - 2010/12/15/revised
PY - 2010/12/22/accepted
PY - 2011/3/3/entrez
PY - 2011/3/3/pubmed
PY - 2011/6/21/medline
SP - 712
EP - 9
JF - Electrophoresis
JO - Electrophoresis
VL - 32
IS - 6-7
N2 - A polyacrylamide-coated CE method with LIF detection was developed for analyzing three phosphoamino acids including phosphotyrosine (p-Tyr), phosphothreonine (p-Thr), and phosphoserine (p-Ser). A near-infrared dye, 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium (MeCy5-OSu) was employed for derivatization of these phosphoamino acids. Results indicated that the complete baseline resolution of each phosphoamino acid was obtained within 6.1 min, using 10 mmol/L phosphate buffer (pH 4.0) containing 60 mmol/L SDS as running buffer. The highest derivatization efficiency was achieved in 0.2 mol/L borate buffer (pH 8.8) for 30 min at 30 °C. Linearity of response was found in the range of 0.05-1 μmol/L. The correlation coefficients for these phosphoamino acids were from 0.9940 to 0.9976. The LODs for phosphotyrosine, phosphothreonine, and phosphoserine were about 6, 8, and 8 nmol/L, respectively. The proposed method has been successfully applied to the determination of phosphoamino acids in the hydrolysis sample of a phosphorylated phosvitin. Average recoveries for phosvitin sample were in the range of 94.0-98.0% and coefficients of variation ranged from 2.7 to 4.8%.
SN - 1522-2683
UR - https://www.unboundmedicine.com/medline/citation/21365656/Rapid_analysis_of_phosphoamino_acids_from_phosvitin_by_near_infrared_cyanine_1__ε_succinimydyl_hexanoate__1'_methyl_333'3'_tetramethyl_indocarbocyanine_55'_disulfonate_potassium_derivatization_and_polyacrylamide_coated_CE_with_LIF_detection_
L2 - https://doi.org/10.1002/elps.201000540
DB - PRIME
DP - Unbound Medicine
ER -
