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Longitudinal profiles of immunoglobulin G antibodies against severe acute respiratory syndrome coronavirus components and neutralizing activities in recovered patients.
Scand J Infect Dis. 2011 Jul; 43(6-7):515-21.SJ

Abstract

BACKGROUND

Immunological memory is the basis for vaccination. Currently, the longitudinal profiles of antibody responses in recovered severe acute respiratory syndrome (SARS) patients have not been fully characterized.

METHODS

In this study we sequentially followed up 19 recovered SARS patients over a 3-y period in order to characterize the dynamic changes in antibody responses against viral components in detail. In addition, 4 blood samples were obtained at month 60.

RESULTS

We found that immunoglobulin G (IgG) antibodies and their neutralizing activities decreased throughout the entire phase of the study. For IgG antibodies in the 3rd y, the positive rate of whole-virus-specific antibodies was 42%, which was tested with commercial kits at 1/10 dilution of the sera. In comparison, the positive rate of spike (S) protein-specific antibodies was 100%, which was tested by spike protein-based ELISA at 1/100 dilution; 4 samples at month 60 were included. The average optical density (OD) reading of nucleocapsid (N) protein-specific antibodies fell dramatically between month 3 and month 12, and it decreased gradually at low levels that were a little higher than the cut-off value from month 12. For neutralizing antibodies, neutralizing activity was detectable in 89% of recovered patients in the 3rd y. S protein-specific IgG levels (r = 0.717) correlated better with neutralizing activity than SARS coronavirus-specific IgG levels (r = 0.571).

CONCLUSIONS

These systematic findings provide valuable information on natural humoral memory responses, and the data will be helpful for understanding the pathogenesis of SARS coronavirus infection and for the rational design of vaccines.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Liu L
Department of Infectious Diseases, Peking Union Medical College Hospital, Beijing, China.
Xie J
No affiliation info available
Sun J
No affiliation info available
Han Y
No affiliation info available
Zhang C
No affiliation info available
Fan H
No affiliation info available
Liu Z
No affiliation info available
Qiu Z
No affiliation info available
He Y
No affiliation info available
Li T
No affiliation info available

MeSH

AdultAntibodies, NeutralizingAntibodies, ViralAntigens, ViralEnzyme-Linked Immunosorbent AssayFemaleFollow-Up StudiesHumansImmunoglobulin GMaleMiddle AgedNeutralization TestsSARS VirusSevere Acute Respiratory SyndromeTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21366405

Citation

Liu, Lifeng, et al. "Longitudinal Profiles of Immunoglobulin G Antibodies Against Severe Acute Respiratory Syndrome Coronavirus Components and Neutralizing Activities in Recovered Patients." Scandinavian Journal of Infectious Diseases, vol. 43, no. 6-7, 2011, pp. 515-21.
Liu L, Xie J, Sun J, et al. Longitudinal profiles of immunoglobulin G antibodies against severe acute respiratory syndrome coronavirus components and neutralizing activities in recovered patients. Scand J Infect Dis. 2011;43(6-7):515-21.
Liu, L., Xie, J., Sun, J., Han, Y., Zhang, C., Fan, H., Liu, Z., Qiu, Z., He, Y., & Li, T. (2011). Longitudinal profiles of immunoglobulin G antibodies against severe acute respiratory syndrome coronavirus components and neutralizing activities in recovered patients. Scandinavian Journal of Infectious Diseases, 43(6-7), 515-21. https://doi.org/10.3109/00365548.2011.560184
Liu L, et al. Longitudinal Profiles of Immunoglobulin G Antibodies Against Severe Acute Respiratory Syndrome Coronavirus Components and Neutralizing Activities in Recovered Patients. Scand J Infect Dis. 2011;43(6-7):515-21. PubMed PMID: 21366405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Longitudinal profiles of immunoglobulin G antibodies against severe acute respiratory syndrome coronavirus components and neutralizing activities in recovered patients. AU - Liu,Lifeng, AU - Xie,Jing, AU - Sun,Jianpin, AU - Han,Yang, AU - Zhang,Chao, AU - Fan,Hongwei, AU - Liu,Zhengyin, AU - Qiu,Zhifeng, AU - He,Yuxian, AU - Li,Taisheng, Y1 - 2011/03/02/ PY - 2011/3/4/entrez PY - 2011/3/4/pubmed PY - 2011/9/29/medline SP - 515 EP - 21 JF - Scandinavian journal of infectious diseases JO - Scand J Infect Dis VL - 43 IS - 6-7 N2 - BACKGROUND: Immunological memory is the basis for vaccination. Currently, the longitudinal profiles of antibody responses in recovered severe acute respiratory syndrome (SARS) patients have not been fully characterized. METHODS: In this study we sequentially followed up 19 recovered SARS patients over a 3-y period in order to characterize the dynamic changes in antibody responses against viral components in detail. In addition, 4 blood samples were obtained at month 60. RESULTS: We found that immunoglobulin G (IgG) antibodies and their neutralizing activities decreased throughout the entire phase of the study. For IgG antibodies in the 3rd y, the positive rate of whole-virus-specific antibodies was 42%, which was tested with commercial kits at 1/10 dilution of the sera. In comparison, the positive rate of spike (S) protein-specific antibodies was 100%, which was tested by spike protein-based ELISA at 1/100 dilution; 4 samples at month 60 were included. The average optical density (OD) reading of nucleocapsid (N) protein-specific antibodies fell dramatically between month 3 and month 12, and it decreased gradually at low levels that were a little higher than the cut-off value from month 12. For neutralizing antibodies, neutralizing activity was detectable in 89% of recovered patients in the 3rd y. S protein-specific IgG levels (r = 0.717) correlated better with neutralizing activity than SARS coronavirus-specific IgG levels (r = 0.571). CONCLUSIONS: These systematic findings provide valuable information on natural humoral memory responses, and the data will be helpful for understanding the pathogenesis of SARS coronavirus infection and for the rational design of vaccines. SN - 1651-1980 UR - https://www.unboundmedicine.com/medline/citation/21366405/Longitudinal_profiles_of_immunoglobulin_G_antibodies_against_severe_acute_respiratory_syndrome_coronavirus_components_and_neutralizing_activities_in_recovered_patients_ DB - PRIME DP - Unbound Medicine ER -