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Oxytocin, vasoactive-intestinal peptide, and serotonin regulate the mating-induced surges of prolactin secretion in the rat.
Endocrinology. 1990 Jan; 126(1):279-84.E

Abstract

In female rats the mating stimulus induces a bimodal pattern of PRL secretion. A surge of PRL occurs at approximately 0300 h, called the nocturnal surge (N). Another surge occurs at 1700 h on the same day, called the diurnal surge (D). By lowering dopaminergic tone pharmacologically, we have recently demonstrated the existence of an endogenous rhythm stimulatory to PRL secretion in female rats. The periods of this stimulatory influence coincide with the periods of the N and D surges of PRL that occur in mated rats. In addition, we have shown that the 0300 h component of this endogenous rhythm is regulated by oxytocin (OT) and vasoactive intestinal peptide (VIP), and the 1700 h component is regulated by OT and serotonin (5-HT). In this study, we investigated the roles of OT, VIP, and 5-HT in controlling the N and D surges of PRL in ovariectomized (OVX) rats receiving a physiological dopamine-lowering stimulus, copulomimetic stimulation. Blood samples were obtained the day before the experiments between 1700 and 1900 h to verify that the rats used were having surges of PRL in response to cervical stimulation (CS). The role of OT was studied by infusing the OT antagonist 1-deamino-2-D-Trp4-Val8-Orn-OT (OT-A; 0.5 micrograms/kg.min) beginning at either 0100 or 1500 h and continuing for 5 h on day 2 after the last CS. Serial blood samples were obtained immediately before infusion and 60, 90, 120, 150, 180, 240, and 300 min after the start of infusion. The samples overlapped either the N or D surge of PRL. All rats used in these studies demonstrated D surges of PRL the day before the experiment. Saline infusion had no effect on either the N or D surge of PRL in OVX-CS rats. However, infusion of OT-A completely blocked both the N and D surges of PRL. The role of VIP was studied by infusing the VIP antagonist [4-D-Cl-Phe6-Leu17]VIP (VIP-A; 01 micrograms/kg.min) beginning at either 0100 or 1500 h and continuing for 5 h. VIP-A completely blocked both the N and D surges of PRL. To study the role of 5-HT, rats received an acute treatment with p-chlorophenylalanine (PCPA; 250 mg/kg, sc) at either 0100 or 1500 h, and blood samples were taken as before. PCPA had no effect on the N surge of PRL.(ABSTRACT TRUNCATED AT 400 WORDS)

Authors+Show Affiliations

Arey BJ
Department of Biological Sciences, Florida State University, Tallahassee 32306.
Freeman ME
No affiliation info available

MeSH

AnimalsCervix UteriCircadian RhythmElectric StimulationFemaleFenclonineOvariectomyOxytocinProlactinRatsRats, Inbred StrainsReproductionSerotoninVasoactive Intestinal Peptide

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2136724

Citation

Arey, B J., and M E. Freeman. "Oxytocin, Vasoactive-intestinal Peptide, and Serotonin Regulate the Mating-induced Surges of Prolactin Secretion in the Rat." Endocrinology, vol. 126, no. 1, 1990, pp. 279-84.
Arey BJ, Freeman ME. Oxytocin, vasoactive-intestinal peptide, and serotonin regulate the mating-induced surges of prolactin secretion in the rat. Endocrinology. 1990;126(1):279-84.
Arey, B. J., & Freeman, M. E. (1990). Oxytocin, vasoactive-intestinal peptide, and serotonin regulate the mating-induced surges of prolactin secretion in the rat. Endocrinology, 126(1), 279-84.
Arey BJ, Freeman ME. Oxytocin, Vasoactive-intestinal Peptide, and Serotonin Regulate the Mating-induced Surges of Prolactin Secretion in the Rat. Endocrinology. 1990;126(1):279-84. PubMed PMID: 2136724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxytocin, vasoactive-intestinal peptide, and serotonin regulate the mating-induced surges of prolactin secretion in the rat. AU - Arey,B J, AU - Freeman,M E, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 279 EP - 84 JF - Endocrinology JO - Endocrinology VL - 126 IS - 1 N2 - In female rats the mating stimulus induces a bimodal pattern of PRL secretion. A surge of PRL occurs at approximately 0300 h, called the nocturnal surge (N). Another surge occurs at 1700 h on the same day, called the diurnal surge (D). By lowering dopaminergic tone pharmacologically, we have recently demonstrated the existence of an endogenous rhythm stimulatory to PRL secretion in female rats. The periods of this stimulatory influence coincide with the periods of the N and D surges of PRL that occur in mated rats. In addition, we have shown that the 0300 h component of this endogenous rhythm is regulated by oxytocin (OT) and vasoactive intestinal peptide (VIP), and the 1700 h component is regulated by OT and serotonin (5-HT). In this study, we investigated the roles of OT, VIP, and 5-HT in controlling the N and D surges of PRL in ovariectomized (OVX) rats receiving a physiological dopamine-lowering stimulus, copulomimetic stimulation. Blood samples were obtained the day before the experiments between 1700 and 1900 h to verify that the rats used were having surges of PRL in response to cervical stimulation (CS). The role of OT was studied by infusing the OT antagonist 1-deamino-2-D-Trp4-Val8-Orn-OT (OT-A; 0.5 micrograms/kg.min) beginning at either 0100 or 1500 h and continuing for 5 h on day 2 after the last CS. Serial blood samples were obtained immediately before infusion and 60, 90, 120, 150, 180, 240, and 300 min after the start of infusion. The samples overlapped either the N or D surge of PRL. All rats used in these studies demonstrated D surges of PRL the day before the experiment. Saline infusion had no effect on either the N or D surge of PRL in OVX-CS rats. However, infusion of OT-A completely blocked both the N and D surges of PRL. The role of VIP was studied by infusing the VIP antagonist [4-D-Cl-Phe6-Leu17]VIP (VIP-A; 01 micrograms/kg.min) beginning at either 0100 or 1500 h and continuing for 5 h. VIP-A completely blocked both the N and D surges of PRL. To study the role of 5-HT, rats received an acute treatment with p-chlorophenylalanine (PCPA; 250 mg/kg, sc) at either 0100 or 1500 h, and blood samples were taken as before. PCPA had no effect on the N surge of PRL.(ABSTRACT TRUNCATED AT 400 WORDS) SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/2136724/Oxytocin_vasoactive_intestinal_peptide_and_serotonin_regulate_the_mating_induced_surges_of_prolactin_secretion_in_the_rat_ DB - PRIME DP - Unbound Medicine ER -