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Antioxidant activity and protective effect of banana peel against oxidative hemolysis of human erythrocyte at different stages of ripening.
Appl Biochem Biotechnol. 2011 Aug; 164(7):1192-206.AB

Abstract

Phytochemicals such as polyphenols and carotenoids are gaining importance because of their contribution to human health and their multiple biological effects such as antioxidant, antimutagenic, anticarcinogenic, and cytoprotective activities and their therapeutic properties. Banana peel is a major by-product in pulp industry and it contains various bioactive compounds like polyphenols, carotenoids, and others. In the present study, effect of ripening, solvent polarity on the content of bioactive compounds of crude banana peel and the protective effect of peel extracts of unripe, ripe, and leaky ripe banana fruit on hydrogen peroxide-induced hemolysis and their antioxidant capacity were investigated. Banana (Musa paradisica) peel at different stages of ripening (unripe, ripe, leaky ripe) were treated with 70% acetone, which were partitioned in order of polarity with water, ethyl acetate, chloroform (CHCl₃), and hexane sequentially. The antioxidant activity of the samples was evaluated by the red cell hemolysis assay, free radical scavenging (1,1-diphenyl-2-picrylhydrazyl free radical elimination) and superoxide dismutase activities. The Folin-Ciocalteu's reagent assay was used to estimate the phenolic content of extracts. The findings of this investigation suggest that the unripe banana peel sample had higher antioxidant potency than ripe and leaky ripe. Further on fractionation, ethyl acetate and water soluble fractions of unripe peel displayed high antioxidant activity than CHCl₃ and hexane fraction, respectively. A positive correlation between free radical scavenging capacity and the content of phenolic compound were found in unripe, ripe, and leaky ripe stages of banana peel.

Authors+Show Affiliations

Centre for Biotechnology, University of Allahabad, Allahabad 211002, India. shanthy_s@rediffmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21369778

Citation

Sundaram, Shanthy, et al. "Antioxidant Activity and Protective Effect of Banana Peel Against Oxidative Hemolysis of Human Erythrocyte at Different Stages of Ripening." Applied Biochemistry and Biotechnology, vol. 164, no. 7, 2011, pp. 1192-206.
Sundaram S, Anjum S, Dwivedi P, et al. Antioxidant activity and protective effect of banana peel against oxidative hemolysis of human erythrocyte at different stages of ripening. Appl Biochem Biotechnol. 2011;164(7):1192-206.
Sundaram, S., Anjum, S., Dwivedi, P., & Rai, G. K. (2011). Antioxidant activity and protective effect of banana peel against oxidative hemolysis of human erythrocyte at different stages of ripening. Applied Biochemistry and Biotechnology, 164(7), 1192-206. https://doi.org/10.1007/s12010-011-9205-3
Sundaram S, et al. Antioxidant Activity and Protective Effect of Banana Peel Against Oxidative Hemolysis of Human Erythrocyte at Different Stages of Ripening. Appl Biochem Biotechnol. 2011;164(7):1192-206. PubMed PMID: 21369778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and protective effect of banana peel against oxidative hemolysis of human erythrocyte at different stages of ripening. AU - Sundaram,Shanthy, AU - Anjum,Shadma, AU - Dwivedi,Priyanka, AU - Rai,Gyanendra Kumar, Y1 - 2011/03/03/ PY - 2010/08/31/received PY - 2011/02/10/accepted PY - 2011/3/4/entrez PY - 2011/3/4/pubmed PY - 2011/10/4/medline SP - 1192 EP - 206 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 164 IS - 7 N2 - Phytochemicals such as polyphenols and carotenoids are gaining importance because of their contribution to human health and their multiple biological effects such as antioxidant, antimutagenic, anticarcinogenic, and cytoprotective activities and their therapeutic properties. Banana peel is a major by-product in pulp industry and it contains various bioactive compounds like polyphenols, carotenoids, and others. In the present study, effect of ripening, solvent polarity on the content of bioactive compounds of crude banana peel and the protective effect of peel extracts of unripe, ripe, and leaky ripe banana fruit on hydrogen peroxide-induced hemolysis and their antioxidant capacity were investigated. Banana (Musa paradisica) peel at different stages of ripening (unripe, ripe, leaky ripe) were treated with 70% acetone, which were partitioned in order of polarity with water, ethyl acetate, chloroform (CHCl₃), and hexane sequentially. The antioxidant activity of the samples was evaluated by the red cell hemolysis assay, free radical scavenging (1,1-diphenyl-2-picrylhydrazyl free radical elimination) and superoxide dismutase activities. The Folin-Ciocalteu's reagent assay was used to estimate the phenolic content of extracts. The findings of this investigation suggest that the unripe banana peel sample had higher antioxidant potency than ripe and leaky ripe. Further on fractionation, ethyl acetate and water soluble fractions of unripe peel displayed high antioxidant activity than CHCl₃ and hexane fraction, respectively. A positive correlation between free radical scavenging capacity and the content of phenolic compound were found in unripe, ripe, and leaky ripe stages of banana peel. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/21369778/Antioxidant_activity_and_protective_effect_of_banana_peel_against_oxidative_hemolysis_of_human_erythrocyte_at_different_stages_of_ripening_ L2 - https://dx.doi.org/10.1007/s12010-011-9205-3 DB - PRIME DP - Unbound Medicine ER -