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The acute toxic effects of particulate matter in mouse lung are related to size and season of collection.
Toxicol Lett. 2011 May 10; 202(3):209-17.TL

Abstract

The toxicity of size-fractionated particulate matter (PM10 and PM2.5) collected in Milano during two different seasons (summer and winter) has been evaluated in vivo. The focus is on time related (3 h, 24 h and 1 week) lung response following a single intratracheal aerosolization in BALB/c mice. The bronchoalveolar lavage fluid (BALf) and the lung parenchyma were screened for different markers of inflammation and cytotoxicity. Histology and immunohistochemistry were performed on excised fixed lungs to assess the effects produced by the different PM fractions. All the analyzed inflammatory markers (PMNs percentage, TNF-α, Hsp70 in the BALf, HO-1 in lung parenchyma), increased after summer PM10 administration; on the contrary winter PM10 and PM2.5 specifically increased the amount of the Cyp1B1, a protein putatively involved in the induction of pro-carcinogenic effect. Moreover, we detected an intensification of LDH activity in the BALf after the administration of winter PM10 and PM2.5, potentially related to an in progress necrotic process while after summer PM10 and PM2.5 administration, the initiation of the caspase cascade suggested a cytotoxic effect sustained by apoptosis. Our results evidenced the toxicity mechanisms elicited by size fractionated PM samples, collected in winter and summer seasons, which differs for dimensions, chemical and microbiological composition. PM10 has been indicated to elicit above all a pro-inflammatory response, linked to its specific biological components, while PM2.5 is supposed to be more harmful due to its smaller dimension and the ability to distribute into the lung alveolar districts. We hypothesized that adverse health effects observed after a single dose of winter PM2.5 is at least partly caused by specific winter PM components, i.e. PAH and transitional metals.

Authors+Show Affiliations

Department of Experimental Medicine, POLARIS Research Center, University of Milano-Bicocca, 48 via Cadore, Monza 20052, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21371539

Citation

Farina, Francesca, et al. "The Acute Toxic Effects of Particulate Matter in Mouse Lung Are Related to Size and Season of Collection." Toxicology Letters, vol. 202, no. 3, 2011, pp. 209-17.
Farina F, Sancini G, Mantecca P, et al. The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol Lett. 2011;202(3):209-17.
Farina, F., Sancini, G., Mantecca, P., Gallinotti, D., Camatini, M., & Palestini, P. (2011). The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicology Letters, 202(3), 209-17. https://doi.org/10.1016/j.toxlet.2011.01.031
Farina F, et al. The Acute Toxic Effects of Particulate Matter in Mouse Lung Are Related to Size and Season of Collection. Toxicol Lett. 2011 May 10;202(3):209-17. PubMed PMID: 21371539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. AU - Farina,Francesca, AU - Sancini,Giulio, AU - Mantecca,Paride, AU - Gallinotti,Daniele, AU - Camatini,Marina, AU - Palestini,Paola, Y1 - 2011/03/01/ PY - 2010/09/24/received PY - 2011/01/27/revised PY - 2011/01/31/accepted PY - 2011/3/5/entrez PY - 2011/3/5/pubmed PY - 2011/6/15/medline SP - 209 EP - 17 JF - Toxicology letters JO - Toxicol Lett VL - 202 IS - 3 N2 - The toxicity of size-fractionated particulate matter (PM10 and PM2.5) collected in Milano during two different seasons (summer and winter) has been evaluated in vivo. The focus is on time related (3 h, 24 h and 1 week) lung response following a single intratracheal aerosolization in BALB/c mice. The bronchoalveolar lavage fluid (BALf) and the lung parenchyma were screened for different markers of inflammation and cytotoxicity. Histology and immunohistochemistry were performed on excised fixed lungs to assess the effects produced by the different PM fractions. All the analyzed inflammatory markers (PMNs percentage, TNF-α, Hsp70 in the BALf, HO-1 in lung parenchyma), increased after summer PM10 administration; on the contrary winter PM10 and PM2.5 specifically increased the amount of the Cyp1B1, a protein putatively involved in the induction of pro-carcinogenic effect. Moreover, we detected an intensification of LDH activity in the BALf after the administration of winter PM10 and PM2.5, potentially related to an in progress necrotic process while after summer PM10 and PM2.5 administration, the initiation of the caspase cascade suggested a cytotoxic effect sustained by apoptosis. Our results evidenced the toxicity mechanisms elicited by size fractionated PM samples, collected in winter and summer seasons, which differs for dimensions, chemical and microbiological composition. PM10 has been indicated to elicit above all a pro-inflammatory response, linked to its specific biological components, while PM2.5 is supposed to be more harmful due to its smaller dimension and the ability to distribute into the lung alveolar districts. We hypothesized that adverse health effects observed after a single dose of winter PM2.5 is at least partly caused by specific winter PM components, i.e. PAH and transitional metals. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/21371539/The_acute_toxic_effects_of_particulate_matter_in_mouse_lung_are_related_to_size_and_season_of_collection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(11)00074-9 DB - PRIME DP - Unbound Medicine ER -