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Differential tolerance of two Gammarus pulex populations transplanted from different metallogenic regions to a polymetal gradient.
Aquat Toxicol. 2011 Mar; 102(1-2):95-103.AT

Abstract

The River Hayle, Cornwall, UK exhibits pronounced Cu and Zn concentration gradients which were used to compare the metal handling abilities of two populations of Gammarus pulex (Crustacea: Amphipoda). One population was native to the Hayle region (Drym) and presumably has been historically impacted by elevated Cu and Zn levels, whilst naïve gammarids were collected from the River Cray, Kent, UK. Both populations were subject to a 32 day in situ exposure at four R. Hayle sites (Drym, Godolphin, Relubbus and St. Erth). Mortality (LT50), Cu and Zn accumulation and sub-cellular distribution, and oxidative stress (malondialdehyde production) increased with the expected Cu and Zn bioavailabilities at the four sites (i.e. Godolphin>Relubbus>St. Erth>Drym). The naïve population experienced greater metal induced effects in terms of Cu and Zn accumulation, oxidative stress responses and lower LT50s. Analysis of Cu and Zn sub-cellular distribution, however, revealed no significant differences in metal handling. In both populations each metal was localised predominantly to the sub-cellular fraction containing metal bound to metallothionein-like proteins (MTLP) or that holding both metal-rich granules (MRG) and exoskeleton, MTLP and MRG binding being indicative of metal detoxification. However, a greater capacity for detoxified metal storage is not a mechanism implicated in the perceived tolerance of the historically impacted gammarids. Instead our results suggest that the historically impacted population was adapted for lower uptake of Cu and Zn leading to lower bioaccumulation, stress response and ultimately mortality. These results demonstrate not only the usefulness of the in situ methodology, but also that differences in population exposure history can cause significant differences in metal responses during exposure at higher concentrations.

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Authors+Show Affiliations

Khan FR
Nutritional Sciences Division, King's College London, 150 Stamford Street, London, United Kingdom. f.khan@nhm.ac.uk
Irving JR
No affiliation info available
Bury NR
No affiliation info available
Hogstrand C
No affiliation info available

MeSH

Adaptation, PhysiologicalAmphipodaAnimal Population GroupsAnimalsBiological AvailabilityCopperCytotoxinsEnglandEnvironmental ExposureInactivation, MetabolicMalondialdehydeMortalityOxidative StressRiversToxicity TestsWater Pollutants, ChemicalZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21371617

Citation

Khan, Farhan R., et al. "Differential Tolerance of Two Gammarus Pulex Populations Transplanted From Different Metallogenic Regions to a Polymetal Gradient." Aquatic Toxicology (Amsterdam, Netherlands), vol. 102, no. 1-2, 2011, pp. 95-103.
Khan FR, Irving JR, Bury NR, et al. Differential tolerance of two Gammarus pulex populations transplanted from different metallogenic regions to a polymetal gradient. Aquat Toxicol. 2011;102(1-2):95-103.
Khan, F. R., Irving, J. R., Bury, N. R., & Hogstrand, C. (2011). Differential tolerance of two Gammarus pulex populations transplanted from different metallogenic regions to a polymetal gradient. Aquatic Toxicology (Amsterdam, Netherlands), 102(1-2), 95-103. https://doi.org/10.1016/j.aquatox.2011.01.001
Khan FR, et al. Differential Tolerance of Two Gammarus Pulex Populations Transplanted From Different Metallogenic Regions to a Polymetal Gradient. Aquat Toxicol. 2011;102(1-2):95-103. PubMed PMID: 21371617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential tolerance of two Gammarus pulex populations transplanted from different metallogenic regions to a polymetal gradient. AU - Khan,Farhan R, AU - Irving,Jennifer R, AU - Bury,Nicolas R, AU - Hogstrand,Christer, Y1 - 2011/01/13/ PY - 2010/09/28/received PY - 2010/12/23/revised PY - 2011/01/04/accepted PY - 2011/3/5/entrez PY - 2011/3/5/pubmed PY - 2011/5/13/medline SP - 95 EP - 103 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 102 IS - 1-2 N2 - The River Hayle, Cornwall, UK exhibits pronounced Cu and Zn concentration gradients which were used to compare the metal handling abilities of two populations of Gammarus pulex (Crustacea: Amphipoda). One population was native to the Hayle region (Drym) and presumably has been historically impacted by elevated Cu and Zn levels, whilst naïve gammarids were collected from the River Cray, Kent, UK. Both populations were subject to a 32 day in situ exposure at four R. Hayle sites (Drym, Godolphin, Relubbus and St. Erth). Mortality (LT50), Cu and Zn accumulation and sub-cellular distribution, and oxidative stress (malondialdehyde production) increased with the expected Cu and Zn bioavailabilities at the four sites (i.e. Godolphin>Relubbus>St. Erth>Drym). The naïve population experienced greater metal induced effects in terms of Cu and Zn accumulation, oxidative stress responses and lower LT50s. Analysis of Cu and Zn sub-cellular distribution, however, revealed no significant differences in metal handling. In both populations each metal was localised predominantly to the sub-cellular fraction containing metal bound to metallothionein-like proteins (MTLP) or that holding both metal-rich granules (MRG) and exoskeleton, MTLP and MRG binding being indicative of metal detoxification. However, a greater capacity for detoxified metal storage is not a mechanism implicated in the perceived tolerance of the historically impacted gammarids. Instead our results suggest that the historically impacted population was adapted for lower uptake of Cu and Zn leading to lower bioaccumulation, stress response and ultimately mortality. These results demonstrate not only the usefulness of the in situ methodology, but also that differences in population exposure history can cause significant differences in metal responses during exposure at higher concentrations. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/21371617/Differential_tolerance_of_two_Gammarus_pulex_populations_transplanted_from_different_metallogenic_regions_to_a_polymetal_gradient_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(11)00002-6 DB - PRIME DP - Unbound Medicine ER -