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A simple assay to amplify the electrochemical signal by the aptamer based biosensor modified with CdS hollow nanospheres.
Biosens Bioelectron. 2011 Apr 15; 26(8):3531-5.BB

Abstract

A simple method to amplify the electrochemical signal by an aptamer with 22 bases modified with CdS hollow nanospheres (CdSHNs) was described. Using the thrombin as a model, the interaction between the aptamer and CdSHNs was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and circular dichroism spectroscopy. CdSHNs promoted the electron transfer between the gold electrode and K(3)[Fe(CN)(6)] and facilitated the conformation conversion of the aptamer from hairpin to G-quadruplex after the aptamer interacted with thrombin. Under optimal conditions, the modified electrode could be used for the determination of thrombin from 0 to 33 μg mL(-1) and the sensitivity was 1.34 μA mL μg(-1)cm(-2), while the linear range of the modified electrode without the immobilization of CdSHNs was from 2.75 to 27.5 μg mL(-1) and the sensitivity was 0.062 μA mL μg(-1)cm(-2). This constructed biosensor also had a good stability, specificity, reproducibility and accuracy which could provide a promising platform for fabrication of aptamer based biosensors.

Authors+Show Affiliations

Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21371879

Citation

Li, Yanfen, et al. "A Simple Assay to Amplify the Electrochemical Signal By the Aptamer Based Biosensor Modified With CdS Hollow Nanospheres." Biosensors & Bioelectronics, vol. 26, no. 8, 2011, pp. 3531-5.
Li Y, Bao J, Han M, et al. A simple assay to amplify the electrochemical signal by the aptamer based biosensor modified with CdS hollow nanospheres. Biosens Bioelectron. 2011;26(8):3531-5.
Li, Y., Bao, J., Han, M., Dai, Z., & Wang, H. (2011). A simple assay to amplify the electrochemical signal by the aptamer based biosensor modified with CdS hollow nanospheres. Biosensors & Bioelectronics, 26(8), 3531-5. https://doi.org/10.1016/j.bios.2011.01.039
Li Y, et al. A Simple Assay to Amplify the Electrochemical Signal By the Aptamer Based Biosensor Modified With CdS Hollow Nanospheres. Biosens Bioelectron. 2011 Apr 15;26(8):3531-5. PubMed PMID: 21371879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple assay to amplify the electrochemical signal by the aptamer based biosensor modified with CdS hollow nanospheres. AU - Li,Yanfen, AU - Bao,Jianchun, AU - Han,Min, AU - Dai,Zhihui, AU - Wang,Huaisheng, Y1 - 2011/03/02/ PY - 2010/11/29/received PY - 2011/01/27/revised PY - 2011/01/31/accepted PY - 2011/3/5/entrez PY - 2011/3/5/pubmed PY - 2011/8/4/medline SP - 3531 EP - 5 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 8 N2 - A simple method to amplify the electrochemical signal by an aptamer with 22 bases modified with CdS hollow nanospheres (CdSHNs) was described. Using the thrombin as a model, the interaction between the aptamer and CdSHNs was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and circular dichroism spectroscopy. CdSHNs promoted the electron transfer between the gold electrode and K(3)[Fe(CN)(6)] and facilitated the conformation conversion of the aptamer from hairpin to G-quadruplex after the aptamer interacted with thrombin. Under optimal conditions, the modified electrode could be used for the determination of thrombin from 0 to 33 μg mL(-1) and the sensitivity was 1.34 μA mL μg(-1)cm(-2), while the linear range of the modified electrode without the immobilization of CdSHNs was from 2.75 to 27.5 μg mL(-1) and the sensitivity was 0.062 μA mL μg(-1)cm(-2). This constructed biosensor also had a good stability, specificity, reproducibility and accuracy which could provide a promising platform for fabrication of aptamer based biosensors. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21371879/A_simple_assay_to_amplify_the_electrochemical_signal_by_the_aptamer_based_biosensor_modified_with_CdS_hollow_nanospheres_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00063-7 DB - PRIME DP - Unbound Medicine ER -