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Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors.
Stem Cells. 2011 May; 29(5):777-90.SC

Abstract

An effective long-term cell therapy for skeletal muscle regeneration requires donor contribution to both muscle fibers and the muscle stem cell pool. Although satellite cells have these abilities, their therapeutic potential so far has been limited due to their scarcity in adult muscle. Myogenic progenitors obtained from Pax3-engineered mouse embryonic stem (ES) cells have the ability to generate myofibers and to improve the contractility of transplanted muscles in vivo, however, whether these cells contribute to the muscle stem cell pool and are able to self-renew in vivo are still unknown. Here, we addressed this question by investigating the ability of Pax3, which plays a critical role in embryonic muscle formation, and Pax7, which is important for maintenance of the muscle satellite cell pool, to promote the derivation of self-renewing functional myogenic progenitors from ES cells. We show that Pax7, like Pax3, can drive the expansion of an ES-derived myogenic progenitor with significant muscle regenerative potential. We further demonstrate that a fraction of transplanted cells remains mononuclear, and displays key features of skeletal muscle stem cells, including satellite cell localization, response to reinjury, and contribution to muscle regeneration in secondary transplantation assays. The ability to engraft, self-renew, and respond to injury provide foundation for the future therapeutic application of ES-derived myogenic progenitors in muscle disorders.

Authors+Show Affiliations

Lillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21374762

Citation

Darabi, Radbod, et al. "Assessment of the Myogenic Stem Cell Compartment Following Transplantation of Pax3/Pax7-induced Embryonic Stem Cell-derived Progenitors." Stem Cells (Dayton, Ohio), vol. 29, no. 5, 2011, pp. 777-90.
Darabi R, Santos FN, Filareto A, et al. Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors. Stem Cells. 2011;29(5):777-90.
Darabi, R., Santos, F. N., Filareto, A., Pan, W., Koene, R., Rudnicki, M. A., Kyba, M., & Perlingeiro, R. C. (2011). Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors. Stem Cells (Dayton, Ohio), 29(5), 777-90. https://doi.org/10.1002/stem.625
Darabi R, et al. Assessment of the Myogenic Stem Cell Compartment Following Transplantation of Pax3/Pax7-induced Embryonic Stem Cell-derived Progenitors. Stem Cells. 2011;29(5):777-90. PubMed PMID: 21374762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors. AU - Darabi,Radbod, AU - Santos,Filipe N C, AU - Filareto,Antonio, AU - Pan,Weihong, AU - Koene,Ryan, AU - Rudnicki,Michael A, AU - Kyba,Michael, AU - Perlingeiro,Rita C R, PY - 2011/3/5/entrez PY - 2011/3/5/pubmed PY - 2011/9/29/medline SP - 777 EP - 90 JF - Stem cells (Dayton, Ohio) JO - Stem Cells VL - 29 IS - 5 N2 - An effective long-term cell therapy for skeletal muscle regeneration requires donor contribution to both muscle fibers and the muscle stem cell pool. Although satellite cells have these abilities, their therapeutic potential so far has been limited due to their scarcity in adult muscle. Myogenic progenitors obtained from Pax3-engineered mouse embryonic stem (ES) cells have the ability to generate myofibers and to improve the contractility of transplanted muscles in vivo, however, whether these cells contribute to the muscle stem cell pool and are able to self-renew in vivo are still unknown. Here, we addressed this question by investigating the ability of Pax3, which plays a critical role in embryonic muscle formation, and Pax7, which is important for maintenance of the muscle satellite cell pool, to promote the derivation of self-renewing functional myogenic progenitors from ES cells. We show that Pax7, like Pax3, can drive the expansion of an ES-derived myogenic progenitor with significant muscle regenerative potential. We further demonstrate that a fraction of transplanted cells remains mononuclear, and displays key features of skeletal muscle stem cells, including satellite cell localization, response to reinjury, and contribution to muscle regeneration in secondary transplantation assays. The ability to engraft, self-renew, and respond to injury provide foundation for the future therapeutic application of ES-derived myogenic progenitors in muscle disorders. SN - 1549-4918 UR - https://www.unboundmedicine.com/medline/citation/21374762/Assessment_of_the_myogenic_stem_cell_compartment_following_transplantation_of_Pax3/Pax7_induced_embryonic_stem_cell_derived_progenitors_ L2 - https://doi.org/10.1002/stem.625 DB - PRIME DP - Unbound Medicine ER -