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[Effect of electroacupuncture on expression of ionotropic glutamate receptor subunits and their genes in lumbar segments of spinal cord in rats with neuropathic pain].
Zhen Ci Yan Jiu. 2010 Dec; 35(6):403-8.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on the expression of ionotropic glutamate receptor (iGluR) subunits and their mRNAs in the lumbar segments of spinal cord in rats with neuropathic pain, so as to explore its underlying mechanism in relieving spinal hyperalgesia.

METHODS

Thirty SD rats were randomly divided into control, model, and EA groups, with 10 rats in each. The spared nerve injury (SNI) model was established by ligature of the sural nerve after cutting off the common peroneal nerve and anterior tibial nerve. EA (2 Hz, 1 mA) was applied to "Huantiao" (GB 30) and "Weizhong" (BL 40) for 30 min, once daily for 7 days. Mechanical pain threshold was detected before and after modeling and before and after EA treatment. The expression levels of N-methyl-d-aspartic acid (NMDA) receptor subunits NR1 and NR 2 B,and AMPA receptor subunit GluR 1 of iGluR and their genes were assayed by Western blot and reverse transcription polymerase chain reaction (RT-PCR) separately.

RESULTS

In comparison with control group, the mechanical pain thresholds were decreased significantly on day 2, 7 and day 14 following modeling in the model group (P < 0.05, P < 0.01). While compared with the model group, the pain threshold was increased considerably on day 14 in the EA group (P < 0.01). Compared with the control group, the expression levels of lumbar spinal cord NR 2 B and NR 2 B mRNA in the model group were increased significantly (P < 0.05), and those of lumbar spinal cord NR 1 and NR 1 mRNA, GluR 1 and GluR 1 mRNA in the model group increased slightly (P > 0.05). In comparison with the model group, the expression levels of lumbar spinal cord NR 2 B and NR 2 B mRNA in the EA group were downregulated remarkably (P < 0.05), and those of lumbar spinal cord NR 1 and NR 1 mRNA, GluR 1 and GluR 1 mRNA in the EA group down-regulated slightly (P > 0.05).

CONCLUSION

EA can significantly suppress pain reaction in rats with neuropathic pain probably through down-regulating the expression of lumbar spinal cord NR 2 B protein and NR 2 B mRNA.

Authors+Show Affiliations

Jiangsu Provincial Key Laboratory of Combined Acupuncture and Drugs, Nanjing University of Chinese Medicine, Nanjing 210029, China. cma1954@126.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21375012

Citation

Ma, Cheng, et al. "[Effect of Electroacupuncture On Expression of Ionotropic Glutamate Receptor Subunits and Their Genes in Lumbar Segments of Spinal Cord in Rats With Neuropathic Pain]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 35, no. 6, 2010, pp. 403-8.
Ma C, Yu L, Yan LP. [Effect of electroacupuncture on expression of ionotropic glutamate receptor subunits and their genes in lumbar segments of spinal cord in rats with neuropathic pain]. Zhen Ci Yan Jiu. 2010;35(6):403-8.
Ma, C., Yu, L., & Yan, L. P. (2010). [Effect of electroacupuncture on expression of ionotropic glutamate receptor subunits and their genes in lumbar segments of spinal cord in rats with neuropathic pain]. Zhen Ci Yan Jiu = Acupuncture Research, 35(6), 403-8.
Ma C, Yu L, Yan LP. [Effect of Electroacupuncture On Expression of Ionotropic Glutamate Receptor Subunits and Their Genes in Lumbar Segments of Spinal Cord in Rats With Neuropathic Pain]. Zhen Ci Yan Jiu. 2010;35(6):403-8. PubMed PMID: 21375012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on expression of ionotropic glutamate receptor subunits and their genes in lumbar segments of spinal cord in rats with neuropathic pain]. AU - Ma,Cheng, AU - Yu,Li, AU - Yan,Li-ping, PY - 2011/3/8/entrez PY - 2011/3/8/pubmed PY - 2011/3/23/medline SP - 403 EP - 8 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 35 IS - 6 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on the expression of ionotropic glutamate receptor (iGluR) subunits and their mRNAs in the lumbar segments of spinal cord in rats with neuropathic pain, so as to explore its underlying mechanism in relieving spinal hyperalgesia. METHODS: Thirty SD rats were randomly divided into control, model, and EA groups, with 10 rats in each. The spared nerve injury (SNI) model was established by ligature of the sural nerve after cutting off the common peroneal nerve and anterior tibial nerve. EA (2 Hz, 1 mA) was applied to "Huantiao" (GB 30) and "Weizhong" (BL 40) for 30 min, once daily for 7 days. Mechanical pain threshold was detected before and after modeling and before and after EA treatment. The expression levels of N-methyl-d-aspartic acid (NMDA) receptor subunits NR1 and NR 2 B,and AMPA receptor subunit GluR 1 of iGluR and their genes were assayed by Western blot and reverse transcription polymerase chain reaction (RT-PCR) separately. RESULTS: In comparison with control group, the mechanical pain thresholds were decreased significantly on day 2, 7 and day 14 following modeling in the model group (P < 0.05, P < 0.01). While compared with the model group, the pain threshold was increased considerably on day 14 in the EA group (P < 0.01). Compared with the control group, the expression levels of lumbar spinal cord NR 2 B and NR 2 B mRNA in the model group were increased significantly (P < 0.05), and those of lumbar spinal cord NR 1 and NR 1 mRNA, GluR 1 and GluR 1 mRNA in the model group increased slightly (P > 0.05). In comparison with the model group, the expression levels of lumbar spinal cord NR 2 B and NR 2 B mRNA in the EA group were downregulated remarkably (P < 0.05), and those of lumbar spinal cord NR 1 and NR 1 mRNA, GluR 1 and GluR 1 mRNA in the EA group down-regulated slightly (P > 0.05). CONCLUSION: EA can significantly suppress pain reaction in rats with neuropathic pain probably through down-regulating the expression of lumbar spinal cord NR 2 B protein and NR 2 B mRNA. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/21375012/[Effect_of_electroacupuncture_on_expression_of_ionotropic_glutamate_receptor_subunits_and_their_genes_in_lumbar_segments_of_spinal_cord_in_rats_with_neuropathic_pain]_ DB - PRIME DP - Unbound Medicine ER -