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[Effect of electroacupuncture on morphological changes and apoptosis of substantia nigra cells in Parkinson's disease rats].

Abstract

OBJECTIVE

To observe the influence of electroacupuncture (EA) of "Fengfu" (GV16) and "Taichong" (LR3) on morphological changes and apoptotic percentage of substantia nigra cells so as to explore its underlying mechanism in relieving Parkinson's disease.

METHODS

Forty Wistar rats were randomly divided into normal control, sham-operation (sham), model and EA groups, with 10 rats in each. The Parkinson's disease model was established by micro-injection of 6-hydroxyl-dopamine (6-OHDA) into the right striatum. EA (2 Hz, 1 mA) was applied to "Fengfu"(GV16) and "Taichong" (LR3) for 30 min, once daily for 2 weeks. HE staining, Nissl staining and immunohistochemical staining were conducted for observing the morphological changes of substantia nigra pars compacta (SNc) and striatum, and the tyrosine hydroxylase(TH) immuno-reaction (IR) positive neurons and nerve fibers. The apoptosis was detected by flow cytometry.

RESULTS

Compared to the normal control and sham groups, the total number of TH-IR positive neurons and Nissl-stained cells, and OD value of TH-IR positive nerve fibers in the SNc on the 6-OHDA-lesioned side were significantly lower in the model group (P < 0.01). Compared with the model group, the aforementioned 3 indexes of the EA group were increased obviously (P < 0.01). The percentage of apoptosis of SNc on the 6-OHDA-lesioned side was obviously lower in the EA group than in the model group (P < 0.01), but still obviously higher than in the normal group and sham group (P < 0.01).

CONCLUSION

Electroacupuncture therapy can significantly increase the number of neurons of substantia nigra and the density of striatum nerve fibers,and reduce the apoptotic percentage of substantia nigra cells in the Parkinsonian rats, which may contribute to its effect in relieving Parkinson's disease.

Authors+Show Affiliations

,

Hubei University of Chinese Medicine, Wuhan 430061, China. wangyanchunonly@yahoo.com.cn

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Source

MeSH

Animals
Apoptosis
Disease Models, Animal
Electroacupuncture
Female
Gene Expression
Humans
Male
Parkinson Disease
Random Allocation
Rats
Rats, Wistar
Substantia Nigra

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21375014

Citation

Wang, Yan-chun, et al. "[Effect of Electroacupuncture On Morphological Changes and Apoptosis of Substantia Nigra Cells in Parkinson's Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 35, no. 6, 2010, pp. 415-21.
Wang YC, Cheng YH, Ma J, et al. [Effect of electroacupuncture on morphological changes and apoptosis of substantia nigra cells in Parkinson's disease rats]. Zhen Ci Yan Jiu. 2010;35(6):415-21.
Wang, Y. C., Cheng, Y. H., Ma, J., Gan, S. Y., Wang, S. J., Zhou, H., ... Shen, F. (2010). [Effect of electroacupuncture on morphological changes and apoptosis of substantia nigra cells in Parkinson's disease rats]. Zhen Ci Yan Jiu = Acupuncture Research, 35(6), pp. 415-21.
Wang YC, et al. [Effect of Electroacupuncture On Morphological Changes and Apoptosis of Substantia Nigra Cells in Parkinson's Disease Rats]. Zhen Ci Yan Jiu. 2010;35(6):415-21. PubMed PMID: 21375014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on morphological changes and apoptosis of substantia nigra cells in Parkinson's disease rats]. AU - Wang,Yan-chun, AU - Cheng,Yu-he, AU - Ma,Jun, AU - Gan,Shui-yong, AU - Wang,Shu-ju, AU - Zhou,Hua, AU - Du,Yan-jun, AU - Yang,Min, AU - Shen,Feng, PY - 2011/3/8/entrez PY - 2011/3/8/pubmed PY - 2011/3/23/medline SP - 415 EP - 21 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 35 IS - 6 N2 - OBJECTIVE: To observe the influence of electroacupuncture (EA) of "Fengfu" (GV16) and "Taichong" (LR3) on morphological changes and apoptotic percentage of substantia nigra cells so as to explore its underlying mechanism in relieving Parkinson's disease. METHODS: Forty Wistar rats were randomly divided into normal control, sham-operation (sham), model and EA groups, with 10 rats in each. The Parkinson's disease model was established by micro-injection of 6-hydroxyl-dopamine (6-OHDA) into the right striatum. EA (2 Hz, 1 mA) was applied to "Fengfu"(GV16) and "Taichong" (LR3) for 30 min, once daily for 2 weeks. HE staining, Nissl staining and immunohistochemical staining were conducted for observing the morphological changes of substantia nigra pars compacta (SNc) and striatum, and the tyrosine hydroxylase(TH) immuno-reaction (IR) positive neurons and nerve fibers. The apoptosis was detected by flow cytometry. RESULTS: Compared to the normal control and sham groups, the total number of TH-IR positive neurons and Nissl-stained cells, and OD value of TH-IR positive nerve fibers in the SNc on the 6-OHDA-lesioned side were significantly lower in the model group (P < 0.01). Compared with the model group, the aforementioned 3 indexes of the EA group were increased obviously (P < 0.01). The percentage of apoptosis of SNc on the 6-OHDA-lesioned side was obviously lower in the EA group than in the model group (P < 0.01), but still obviously higher than in the normal group and sham group (P < 0.01). CONCLUSION: Electroacupuncture therapy can significantly increase the number of neurons of substantia nigra and the density of striatum nerve fibers,and reduce the apoptotic percentage of substantia nigra cells in the Parkinsonian rats, which may contribute to its effect in relieving Parkinson's disease. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/21375014/[Effect_of_electroacupuncture_on_morphological_changes_and_apoptosis_of_substantia_nigra_cells_in_Parkinson's_disease_rats]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -