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Biosynthesis of very long-chain fatty acids (C>24) in Atlantic salmon: cloning, functional characterisation, and tissue distribution of an Elovl4 elongase.

Abstract

The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step of the elongation process in fatty acid (FA) biosynthesis in vertebrates. One member of the Elovl family, Elovl4, has been regarded as a critical enzyme in vertebrates in the production of the so-called very long-chain fatty acids (VLC-FA), a group of compounds that has been scarcely explored in fish. Here we report on the cloning of a novel Elovl4-like elongase from Atlantic salmon (Salmo salar). The salmon Elovl4 cDNA codes for a putative protein containing 306 amino acids. Heterologous expression in yeast demonstrated that salmon Elovl4 efficiently elongated saturated FAs up to 36:0, with 24:0 and 26:0 appearing as preferred substrates. Additionally, salmon Elovl4 effectively converted C20 and C22 polyunsaturated fatty acids to elongated polyenoic products up to C36. Tissue distribution showed that Elovl4 mRNA transcripts are abundant in eye, brain and testes, suggesting that, as described in mammals, these tissues are important metabolic sites for the biosynthesis of VLC-FA. Our results are discussed in comparison with the functional analyses observed in Elovl4 proteins from other vertebrates, and also other Elovl proteins investigated previously in Atlantic salmon.

Authors+Show Affiliations

Institute of Aquaculture, School of Natural Sciences, University of Stirling, Scotland, UK. g.e.carmonaantonanzas@stir.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21377536

Citation

Carmona-Antoñanzas, Greta, et al. "Biosynthesis of Very Long-chain Fatty Acids (C>24) in Atlantic Salmon: Cloning, Functional Characterisation, and Tissue Distribution of an Elovl4 Elongase." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 159, no. 2, 2011, pp. 122-9.
Carmona-Antoñanzas G, Monroig O, Dick JR, et al. Biosynthesis of very long-chain fatty acids (C>24) in Atlantic salmon: cloning, functional characterisation, and tissue distribution of an Elovl4 elongase. Comp Biochem Physiol B, Biochem Mol Biol. 2011;159(2):122-9.
Carmona-Antoñanzas, G., Monroig, O., Dick, J. R., Davie, A., & Tocher, D. R. (2011). Biosynthesis of very long-chain fatty acids (C>24) in Atlantic salmon: cloning, functional characterisation, and tissue distribution of an Elovl4 elongase. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 159(2), pp. 122-9. doi:10.1016/j.cbpb.2011.02.007.
Carmona-Antoñanzas G, et al. Biosynthesis of Very Long-chain Fatty Acids (C>24) in Atlantic Salmon: Cloning, Functional Characterisation, and Tissue Distribution of an Elovl4 Elongase. Comp Biochem Physiol B, Biochem Mol Biol. 2011;159(2):122-9. PubMed PMID: 21377536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biosynthesis of very long-chain fatty acids (C>24) in Atlantic salmon: cloning, functional characterisation, and tissue distribution of an Elovl4 elongase. AU - Carmona-Antoñanzas,Greta, AU - Monroig,Oscar, AU - Dick,James R, AU - Davie,Andrew, AU - Tocher,Douglas R, Y1 - 2011/03/04/ PY - 2011/01/27/received PY - 2011/02/28/revised PY - 2011/02/28/accepted PY - 2011/3/8/entrez PY - 2011/3/8/pubmed PY - 2011/8/4/medline SP - 122 EP - 9 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 159 IS - 2 N2 - The elongases of very long-chain fatty acids (Elovl) account for the rate-limiting condensation step of the elongation process in fatty acid (FA) biosynthesis in vertebrates. One member of the Elovl family, Elovl4, has been regarded as a critical enzyme in vertebrates in the production of the so-called very long-chain fatty acids (VLC-FA), a group of compounds that has been scarcely explored in fish. Here we report on the cloning of a novel Elovl4-like elongase from Atlantic salmon (Salmo salar). The salmon Elovl4 cDNA codes for a putative protein containing 306 amino acids. Heterologous expression in yeast demonstrated that salmon Elovl4 efficiently elongated saturated FAs up to 36:0, with 24:0 and 26:0 appearing as preferred substrates. Additionally, salmon Elovl4 effectively converted C20 and C22 polyunsaturated fatty acids to elongated polyenoic products up to C36. Tissue distribution showed that Elovl4 mRNA transcripts are abundant in eye, brain and testes, suggesting that, as described in mammals, these tissues are important metabolic sites for the biosynthesis of VLC-FA. Our results are discussed in comparison with the functional analyses observed in Elovl4 proteins from other vertebrates, and also other Elovl proteins investigated previously in Atlantic salmon. SN - 1879-1107 UR - https://www.unboundmedicine.com/medline/citation/21377536/Biosynthesis_of_very_long_chain_fatty_acids__C>24__in_Atlantic_salmon:_cloning_functional_characterisation_and_tissue_distribution_of_an_Elovl4_elongase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096-4959(11)00045-5 DB - PRIME DP - Unbound Medicine ER -