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Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bert.
Exp Toxicol Pathol. 2012 Nov; 64(7-8):807-11.ET

Abstract

In the present study, we carried out a systematic research on relative antioxidant activity of aqueous leaf extract of Stevia rebaudiana. The DPPH activity of aqueous leaf extract (20, 40, 50, 100 and 200 μg/ml) was increased in a dose dependent manner, which was found in the range of 40.00-72.37% as compared to ascorbic acid 64.26-82.58%. The IC(50) values of aqueous extract and ascorbic acid in DPPH radical scavenging assay were obtained to be 83.45 and 26.75 μg/ml, respectively. Measurement of total phenolic content of the aqueous leaf extract of S. rebaudiana was achieved using Folin-Ciocalteau reagent containing 56.73 mg/g of phenolic content, which was found significantly potent when compared to reference standard gallic acid. The aqueous extract also inhibited the hydroxyl radical, nitric oxide and superoxide anions with IC(50) values of 100.86, 98.73 and 100.86 μg/ml, respectively. The greater amount of phenolic compounds leads to more potent radical scavenging effects as shown by the aqueous leaf extract of S. rebaudiana.

Authors+Show Affiliations

Department of Food Science and Technology, Yeungnam University, 214-1 Dae-dong, Gyeongsan-si, Gyeongsangbuk-do 712-749, Republic of Korea. shruti.shukla15@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21377849

Citation

Shukla, Shruti, et al. "Antioxidant Ability and Total Phenolic Content of Aqueous Leaf Extract of Stevia Rebaudiana Bert." Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, vol. 64, no. 7-8, 2012, pp. 807-11.
Shukla S, Mehta A, Mehta P, et al. Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bert. Exp Toxicol Pathol. 2012;64(7-8):807-11.
Shukla, S., Mehta, A., Mehta, P., & Bajpai, V. K. (2012). Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bert. Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, 64(7-8), 807-11. https://doi.org/10.1016/j.etp.2011.02.002
Shukla S, et al. Antioxidant Ability and Total Phenolic Content of Aqueous Leaf Extract of Stevia Rebaudiana Bert. Exp Toxicol Pathol. 2012;64(7-8):807-11. PubMed PMID: 21377849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant ability and total phenolic content of aqueous leaf extract of Stevia rebaudiana Bert. AU - Shukla,Shruti, AU - Mehta,Archana, AU - Mehta,Pradeep, AU - Bajpai,Vivek K, Y1 - 2011/03/05/ PY - 2010/09/16/received PY - 2011/02/08/accepted PY - 2011/3/8/entrez PY - 2011/3/8/pubmed PY - 2013/3/27/medline SP - 807 EP - 11 JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie JO - Exp. Toxicol. Pathol. VL - 64 IS - 7-8 N2 - In the present study, we carried out a systematic research on relative antioxidant activity of aqueous leaf extract of Stevia rebaudiana. The DPPH activity of aqueous leaf extract (20, 40, 50, 100 and 200 μg/ml) was increased in a dose dependent manner, which was found in the range of 40.00-72.37% as compared to ascorbic acid 64.26-82.58%. The IC(50) values of aqueous extract and ascorbic acid in DPPH radical scavenging assay were obtained to be 83.45 and 26.75 μg/ml, respectively. Measurement of total phenolic content of the aqueous leaf extract of S. rebaudiana was achieved using Folin-Ciocalteau reagent containing 56.73 mg/g of phenolic content, which was found significantly potent when compared to reference standard gallic acid. The aqueous extract also inhibited the hydroxyl radical, nitric oxide and superoxide anions with IC(50) values of 100.86, 98.73 and 100.86 μg/ml, respectively. The greater amount of phenolic compounds leads to more potent radical scavenging effects as shown by the aqueous leaf extract of S. rebaudiana. SN - 1618-1433 UR - https://www.unboundmedicine.com/medline/citation/21377849/Antioxidant_ability_and_total_phenolic_content_of_aqueous_leaf_extract_of_Stevia_rebaudiana_Bert_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0940-2993(11)00022-4 DB - PRIME DP - Unbound Medicine ER -