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Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry.
J Breath Res. 2010 Dec; 4(4):046001.JB

Abstract

Using selected ion flow tube-mass spectrometry (SIFT-MS) for on-line analysis, we aimed to define the optimal single-exhalation breathing manoeuvre from which a measure of expired acetone concentration could be obtained. Using known acetone concentrations in vitro, we determined the instrument's accuracy, inter-measurement variability and dynamic response time. Further, we determined the effects of expiratory flow and volume on acetone concentration in the breath of 12 volunteers and calculated intra-individual coefficients of variation (CVs). At acetone concentrations of 600-3000 ppb on 30 days over 2 months there was an instrument measurement bias of 8% that did not change over time, inter-day and intra-day CVs were 5.6% and 0.0%, respectively, and the 10-90% response time was 500 ± 50 ms (mean ± SE). Acetone concentrations at exhalation flows of 193 ± 18 (mean ± SD) and 313 ± 32 ml s(-1) were 619 ± 1.83 (geometric mean ± logSD) and 618 ± 1.82 ppb in the fraction 70-85% by volume of exhaled vital capacity (V(70-85%)) and 636 ± 1.82 (geometric mean ± logSD) and 631 ± 1.83 ppb in V(85-100%). A difference was observed between acetone concentrations in the V(70-85%) and V(85-100%) fractions (p < 0.01), but flow had no effect. Median intra-individual CVs were 1.6-2.6%. On-line SIFT-MS measurement of acetone concentration in a single exhalation requires control of exhaled volume but not flow, and yields low intra-individual CVs and is potentially useful in approximating blood glucose and monitoring metabolic stress.

Authors+Show Affiliations

Respiratory Services, Christchurch Hospital, Christchurch, New Zealand. jack.dummer@otago.ac.nzNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21383484

Citation

Dummer, Jack F., et al. "Accurate, Reproducible Measurement of Acetone Concentration in Breath Using Selected Ion Flow Tube-mass Spectrometry." Journal of Breath Research, vol. 4, no. 4, 2010, p. 046001.
Dummer JF, Storer MK, Hu WP, et al. Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry. J Breath Res. 2010;4(4):046001.
Dummer, J. F., Storer, M. K., Hu, W. P., Swanney, M. P., Milne, G. J., Frampton, C. M., Scotter, J. M., Prisk, G. K., & Epton, M. J. (2010). Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry. Journal of Breath Research, 4(4), 046001. https://doi.org/10.1088/1752-7155/4/4/046001
Dummer JF, et al. Accurate, Reproducible Measurement of Acetone Concentration in Breath Using Selected Ion Flow Tube-mass Spectrometry. J Breath Res. 2010;4(4):046001. PubMed PMID: 21383484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accurate, reproducible measurement of acetone concentration in breath using selected ion flow tube-mass spectrometry. AU - Dummer,Jack F, AU - Storer,Malina K, AU - Hu,Wan-Ping, AU - Swanney,Maureen P, AU - Milne,Gordon J, AU - Frampton,Christopher M, AU - Scotter,Jenny M, AU - Prisk,G Kim, AU - Epton,Michael J, Y1 - 2010/11/15/ PY - 2011/3/9/entrez PY - 2011/3/9/pubmed PY - 2011/7/27/medline SP - 046001 EP - 046001 JF - Journal of breath research JO - J Breath Res VL - 4 IS - 4 N2 - Using selected ion flow tube-mass spectrometry (SIFT-MS) for on-line analysis, we aimed to define the optimal single-exhalation breathing manoeuvre from which a measure of expired acetone concentration could be obtained. Using known acetone concentrations in vitro, we determined the instrument's accuracy, inter-measurement variability and dynamic response time. Further, we determined the effects of expiratory flow and volume on acetone concentration in the breath of 12 volunteers and calculated intra-individual coefficients of variation (CVs). At acetone concentrations of 600-3000 ppb on 30 days over 2 months there was an instrument measurement bias of 8% that did not change over time, inter-day and intra-day CVs were 5.6% and 0.0%, respectively, and the 10-90% response time was 500 ± 50 ms (mean ± SE). Acetone concentrations at exhalation flows of 193 ± 18 (mean ± SD) and 313 ± 32 ml s(-1) were 619 ± 1.83 (geometric mean ± logSD) and 618 ± 1.82 ppb in the fraction 70-85% by volume of exhaled vital capacity (V(70-85%)) and 636 ± 1.82 (geometric mean ± logSD) and 631 ± 1.83 ppb in V(85-100%). A difference was observed between acetone concentrations in the V(70-85%) and V(85-100%) fractions (p < 0.01), but flow had no effect. Median intra-individual CVs were 1.6-2.6%. On-line SIFT-MS measurement of acetone concentration in a single exhalation requires control of exhaled volume but not flow, and yields low intra-individual CVs and is potentially useful in approximating blood glucose and monitoring metabolic stress. SN - 1752-7163 UR - https://www.unboundmedicine.com/medline/citation/21383484/Accurate_reproducible_measurement_of_acetone_concentration_in_breath_using_selected_ion_flow_tube_mass_spectrometry_ L2 - https://doi.org/10.1088/1752-7155/4/4/046001 DB - PRIME DP - Unbound Medicine ER -