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Applicability of SSR markers to the traceability of monovarietal olive oils.
J Sci Food Agric. 2011 Jun; 91(8):1381-91.JS

Abstract

BACKGROUND

To protect the features and authenticity of food products, the European Commission enforces two certification labels: Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI). EEC Regulation No. 510/2006 imposes criteria for labelling, production and commercialisation of olive oil. Since plant genotype is a major determinant in establishing the PDO and PGI labels, methods to ascertain the varieties present in a batch of olive oil are essential in validating product conformity. The traceability of olive oil can be assessed through simple sequence repeat (SSR) co-dominant markers targeted to specific regions of DNA from olive cultivars.

RESULTS

Twenty-one monovarietal olive oils were analysed with nine nuclear and two shortened SSRs. For each marker the correspondence of allelic profile with the reference cultivar, the reproducibility of profiles in different DNA extractions and the polymorphism information content were determined.

CONCLUSION

The results showed that using a panel of SSR markers such as those described in this paper allows one to make a reliable attribution of an olive oil to a specific cultivar.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Vietina M
Division of Genetics and Environmental Biotechnology, Department of Environmental Sciences, University of Parma, Via G.P. Usberti 11/A, I-43100 Parma, Italy.
Agrimonti C
No affiliation info available
Marmiroli M
No affiliation info available
Bonas U
No affiliation info available
Marmiroli N
No affiliation info available

MeSH

AllelesDNA, PlantEuropeFood LabelingGenetic MarkersGenotypeMicrosatellite RepeatsOleaOlive OilPlant OilsPolymorphism, GeneticSpecies Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21384371

Citation

Vietina, Michelangelo, et al. "Applicability of SSR Markers to the Traceability of Monovarietal Olive Oils." Journal of the Science of Food and Agriculture, vol. 91, no. 8, 2011, pp. 1381-91.
Vietina M, Agrimonti C, Marmiroli M, et al. Applicability of SSR markers to the traceability of monovarietal olive oils. J Sci Food Agric. 2011;91(8):1381-91.
Vietina, M., Agrimonti, C., Marmiroli, M., Bonas, U., & Marmiroli, N. (2011). Applicability of SSR markers to the traceability of monovarietal olive oils. Journal of the Science of Food and Agriculture, 91(8), 1381-91. https://doi.org/10.1002/jsfa.4317
Vietina M, et al. Applicability of SSR Markers to the Traceability of Monovarietal Olive Oils. J Sci Food Agric. 2011;91(8):1381-91. PubMed PMID: 21384371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Applicability of SSR markers to the traceability of monovarietal olive oils. AU - Vietina,Michelangelo, AU - Agrimonti,Caterina, AU - Marmiroli,Marta, AU - Bonas,Urbana, AU - Marmiroli,Nelson, Y1 - 2011/03/07/ PY - 2010/07/27/received PY - 2010/12/09/revised PY - 2010/12/20/accepted PY - 2011/3/9/entrez PY - 2011/3/9/pubmed PY - 2011/12/13/medline SP - 1381 EP - 91 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 91 IS - 8 N2 - BACKGROUND: To protect the features and authenticity of food products, the European Commission enforces two certification labels: Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI). EEC Regulation No. 510/2006 imposes criteria for labelling, production and commercialisation of olive oil. Since plant genotype is a major determinant in establishing the PDO and PGI labels, methods to ascertain the varieties present in a batch of olive oil are essential in validating product conformity. The traceability of olive oil can be assessed through simple sequence repeat (SSR) co-dominant markers targeted to specific regions of DNA from olive cultivars. RESULTS: Twenty-one monovarietal olive oils were analysed with nine nuclear and two shortened SSRs. For each marker the correspondence of allelic profile with the reference cultivar, the reproducibility of profiles in different DNA extractions and the polymorphism information content were determined. CONCLUSION: The results showed that using a panel of SSR markers such as those described in this paper allows one to make a reliable attribution of an olive oil to a specific cultivar. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/21384371/Applicability_of_SSR_markers_to_the_traceability_of_monovarietal_olive_oils_ L2 - https://doi.org/10.1002/jsfa.4317 DB - PRIME DP - Unbound Medicine ER -