Tags

Type your tag names separated by a space and hit enter

Analysis of Staphylococcal cassette chromosome mec in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7).
Microb Drug Resist. 2011 Jun; 17(2):291-7.MD

Abstract

Methicillin resistance in staphylococci is conferred by the acquisition in its chromosome of the mecA gene, which is located on a mobile genetic element called staphylococcal cassette chromosome mec (SCCmec). Genetic type of SCCmec is defined by combination of mec gene complex class and cassette chromosome recombinase gene (ccr) allotype. In this study, we analyzed genetic diversity of the SCCmec in 11 Staphylococcus haemolyticus strains and a Staphylococcus sciuri strain, which were recently isolated from clinical specimens in Bangladesh. Among these strains, only two S. haemolyticus strains were proved to have the known types of SCCmec, that is, SCCmec V (class C2 mec-ccrC) and VII (class C1 mec-ccrC). Five S. haemolyticus strains were assigned two unique mec-ccr gene complexes combination; that is, class C1 mec-ccrA4B4 (four isolates) and class A mec-ccrC (one isolate). In the remaining four S. haemolyticus strains with class C1 mec, no known ccr allotypes could be detected. A single S. sciuri strain with class A mec complex carried a ccrA gene belonging to a novel allotype designated ccrA7, together with ccrB3. The ccrA7 gene in the S. sciuri strain showed 61.7%-82.7% sequence identity to the ccrA gene sequences published so far, and 75.3% identity to ccrA3, which is a component of the type 3 ccr complex (ccrA3-ccrB3) in methicillin-resistant Staphylococcus aureus. The results of the present study indicated that mec gene complex and ccr genes in coagulase-negative staphylococci are highly divergent, and distinct from those of common methicillin-resistant S. aureus. Identification of the novel ccrA7 allotype combined with ccrB3 suggested an occurrence of recombination between different ccr complexes in nature.

Authors+Show Affiliations

Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan. noriko-u@sapmed.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21388304

Citation

Urushibara, Noriko, et al. "Analysis of Staphylococcal Cassette Chromosome Mec in Staphylococcus Haemolyticus and Staphylococcus Sciuri: Identification of a Novel Ccr Gene Complex With a Newly Identified ccrA Allotype (ccrA7)." Microbial Drug Resistance (Larchmont, N.Y.), vol. 17, no. 2, 2011, pp. 291-7.
Urushibara N, Paul SK, Hossain MA, et al. Analysis of Staphylococcal cassette chromosome mec in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7). Microb Drug Resist. 2011;17(2):291-7.
Urushibara, N., Paul, S. K., Hossain, M. A., Kawaguchiya, M., & Kobayashi, N. (2011). Analysis of Staphylococcal cassette chromosome mec in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7). Microbial Drug Resistance (Larchmont, N.Y.), 17(2), 291-7. https://doi.org/10.1089/mdr.2010.0144
Urushibara N, et al. Analysis of Staphylococcal Cassette Chromosome Mec in Staphylococcus Haemolyticus and Staphylococcus Sciuri: Identification of a Novel Ccr Gene Complex With a Newly Identified ccrA Allotype (ccrA7). Microb Drug Resist. 2011;17(2):291-7. PubMed PMID: 21388304.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Staphylococcal cassette chromosome mec in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7). AU - Urushibara,Noriko, AU - Paul,Shyamal Kumar, AU - Hossain,Mohammad Akram, AU - Kawaguchiya,Mitsuyo, AU - Kobayashi,Nobumichi, Y1 - 2011/03/09/ PY - 2011/3/11/entrez PY - 2011/3/11/pubmed PY - 2011/9/29/medline SP - 291 EP - 7 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb Drug Resist VL - 17 IS - 2 N2 - Methicillin resistance in staphylococci is conferred by the acquisition in its chromosome of the mecA gene, which is located on a mobile genetic element called staphylococcal cassette chromosome mec (SCCmec). Genetic type of SCCmec is defined by combination of mec gene complex class and cassette chromosome recombinase gene (ccr) allotype. In this study, we analyzed genetic diversity of the SCCmec in 11 Staphylococcus haemolyticus strains and a Staphylococcus sciuri strain, which were recently isolated from clinical specimens in Bangladesh. Among these strains, only two S. haemolyticus strains were proved to have the known types of SCCmec, that is, SCCmec V (class C2 mec-ccrC) and VII (class C1 mec-ccrC). Five S. haemolyticus strains were assigned two unique mec-ccr gene complexes combination; that is, class C1 mec-ccrA4B4 (four isolates) and class A mec-ccrC (one isolate). In the remaining four S. haemolyticus strains with class C1 mec, no known ccr allotypes could be detected. A single S. sciuri strain with class A mec complex carried a ccrA gene belonging to a novel allotype designated ccrA7, together with ccrB3. The ccrA7 gene in the S. sciuri strain showed 61.7%-82.7% sequence identity to the ccrA gene sequences published so far, and 75.3% identity to ccrA3, which is a component of the type 3 ccr complex (ccrA3-ccrB3) in methicillin-resistant Staphylococcus aureus. The results of the present study indicated that mec gene complex and ccr genes in coagulase-negative staphylococci are highly divergent, and distinct from those of common methicillin-resistant S. aureus. Identification of the novel ccrA7 allotype combined with ccrB3 suggested an occurrence of recombination between different ccr complexes in nature. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/21388304/Analysis_of_Staphylococcal_cassette_chromosome_mec_in_Staphylococcus_haemolyticus_and_Staphylococcus_sciuri:_identification_of_a_novel_ccr_gene_complex_with_a_newly_identified_ccrA_allotype__ccrA7__ L2 - https://www.liebertpub.com/doi/10.1089/mdr.2010.0144?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -