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Characterization and use of monoclonal antibodies to identify Haemophilus paragallinarum serovars.

Abstract

Two serovar-specific monoclonal antibodies (MAbs) to Haemophilus paragallinarum serovars A/1 and C/2 strains, respectively, were developed and characterized by hemagglutination-inhibition (HI) and dot-blotting tests using representative H. paragallinarum serovars A/1, B, and C/2 strains. In both the HI and dot-blotting tests, one MAb (E5C12D10), raised against strain 221, serovar A/1, reacted only with serovar A/1 strains, while the other MAb (F2E6), raised against strain S1 of serovar C/2, reacted with only serovar C/2 strains examined. In both tests, the two MAbs did not react with two serovar B strains. These results indicated that the two MAbs recognize serovar-specific hemagglutinating (HA) antigens of H. paragallinarum serovars A/1 and C/2 strains, respectively, and that a dot-blotting test using these MAbs is a practical alternative to the HI test for serotyping H. paragallinarum. Strains 0222 and Spross of serovar B, which did not react with these two MAbs, were found to possess serovar-specific HA antigen in cross-HI tests.

Authors+Show Affiliations

,

Aburahi Laboratories, Shionogi Research Laboratories, Shionogi & Co., Ltd., Shiga, Japan.

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Source

Avian diseases 34:1 pg 52-7

MeSH

Animals
Antibodies, Bacterial
Antibodies, Monoclonal
Antigens, Bacterial
Chickens
Female
Haemophilus
Hemagglutination Inhibition Tests
Immune Sera
Immunoblotting
Mice
Serotyping
Specific Pathogen-Free Organisms

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2138887

Citation

Yamaguchi, T, et al. "Characterization and Use of Monoclonal Antibodies to Identify Haemophilus Paragallinarum Serovars." Avian Diseases, vol. 34, no. 1, 1990, pp. 52-7.
Yamaguchi T, Takigami S, Iritani Y, et al. Characterization and use of monoclonal antibodies to identify Haemophilus paragallinarum serovars. Avian Dis. 1990;34(1):52-7.
Yamaguchi, T., Takigami, S., Iritani, Y., & Hayashi, Y. (1990). Characterization and use of monoclonal antibodies to identify Haemophilus paragallinarum serovars. Avian Diseases, 34(1), pp. 52-7.
Yamaguchi T, et al. Characterization and Use of Monoclonal Antibodies to Identify Haemophilus Paragallinarum Serovars. Avian Dis. 1990;34(1):52-7. PubMed PMID: 2138887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and use of monoclonal antibodies to identify Haemophilus paragallinarum serovars. AU - Yamaguchi,T, AU - Takigami,S, AU - Iritani,Y, AU - Hayashi,Y, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 52 EP - 7 JF - Avian diseases JO - Avian Dis. VL - 34 IS - 1 N2 - Two serovar-specific monoclonal antibodies (MAbs) to Haemophilus paragallinarum serovars A/1 and C/2 strains, respectively, were developed and characterized by hemagglutination-inhibition (HI) and dot-blotting tests using representative H. paragallinarum serovars A/1, B, and C/2 strains. In both the HI and dot-blotting tests, one MAb (E5C12D10), raised against strain 221, serovar A/1, reacted only with serovar A/1 strains, while the other MAb (F2E6), raised against strain S1 of serovar C/2, reacted with only serovar C/2 strains examined. In both tests, the two MAbs did not react with two serovar B strains. These results indicated that the two MAbs recognize serovar-specific hemagglutinating (HA) antigens of H. paragallinarum serovars A/1 and C/2 strains, respectively, and that a dot-blotting test using these MAbs is a practical alternative to the HI test for serotyping H. paragallinarum. Strains 0222 and Spross of serovar B, which did not react with these two MAbs, were found to possess serovar-specific HA antigen in cross-HI tests. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/2138887/Characterization_and_use_of_monoclonal_antibodies_to_identify_Haemophilus_paragallinarum_serovars_ DB - PRIME DP - Unbound Medicine ER -