Tags

Type your tag names separated by a space and hit enter

Ketamine depresses toll-like receptor 3 signaling in spinal microglia in a rat model of neuropathic pain.
Neurosignals. 2011; 19(1):44-53.N

Abstract

Reports suggest that microglia play a key role in spinal nerve ligation (SNL)-induced neuropathic pain, and toll-like receptor 3 (TLR3) has a substantial role in the activation of spinal microglia and the development of tactile allodynia after nerve injury. In addition, ketamine application could suppress microglial activation in vitro, and ketamine could inhibit proinflammatory gene expression possibly by suppressing TLR-mediated signal transduction. Therefore, the present study was designed to disclose whether intrathecal ketamine could suppress SNL-induced spinal microglial activation and exert some antiallodynic effects on neuropathic pain by suppressing TLR3 activation. Behavioral results showed that intrathecal ketamine attenuated SNL-induced mechanical allodynia, as well as spinal microglial activation, in a dose-dependent manner. Furthermore, Western blot analysis displayed that ketamine application downregulated SNL-induced phosphorylated-p38 (p-p38) expression, which was specifically expressed in spinal microglia but not in astrocytes or neurons. Besides, ketamine could reverse TLR3 agonist (polyinosine-polycytidylic acid)-induced mechanical allodynia and spinal microglia activation. It was concluded that intrathecal ketamine depresses TLR3-induced spinal microglial p-p38 mitogen-activated protein kinase pathway activation after SNL, probably contributing to the antiallodynic effect of ketamine on SNL-induced neuropathic pain.

Authors+Show Affiliations

Department of Anesthesiology, School of Stomatology, Fourth Military Medical University, Xi'an, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21389680

Citation

Mei, Xiao-Peng, et al. "Ketamine Depresses Toll-like Receptor 3 Signaling in Spinal Microglia in a Rat Model of Neuropathic Pain." Neuro-Signals, vol. 19, no. 1, 2011, pp. 44-53.
Mei XP, Zhou Y, Wang W, et al. Ketamine depresses toll-like receptor 3 signaling in spinal microglia in a rat model of neuropathic pain. Neurosignals. 2011;19(1):44-53.
Mei, X. P., Zhou, Y., Wang, W., Tang, J., Wang, W., Zhang, H., Xu, L. X., & Li, Y. Q. (2011). Ketamine depresses toll-like receptor 3 signaling in spinal microglia in a rat model of neuropathic pain. Neuro-Signals, 19(1), 44-53. https://doi.org/10.1159/000324293
Mei XP, et al. Ketamine Depresses Toll-like Receptor 3 Signaling in Spinal Microglia in a Rat Model of Neuropathic Pain. Neurosignals. 2011;19(1):44-53. PubMed PMID: 21389680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ketamine depresses toll-like receptor 3 signaling in spinal microglia in a rat model of neuropathic pain. AU - Mei,Xiao-Peng, AU - Zhou,Yang, AU - Wang,Wei, AU - Tang,Jun, AU - Wang,Wen, AU - Zhang,Hui, AU - Xu,Li-Xian, AU - Li,Yun-Qing, Y1 - 2011/03/09/ PY - 2010/11/29/received PY - 2011/01/14/accepted PY - 2011/3/11/entrez PY - 2011/3/11/pubmed PY - 2011/9/20/medline SP - 44 EP - 53 JF - Neuro-Signals JO - Neurosignals VL - 19 IS - 1 N2 - Reports suggest that microglia play a key role in spinal nerve ligation (SNL)-induced neuropathic pain, and toll-like receptor 3 (TLR3) has a substantial role in the activation of spinal microglia and the development of tactile allodynia after nerve injury. In addition, ketamine application could suppress microglial activation in vitro, and ketamine could inhibit proinflammatory gene expression possibly by suppressing TLR-mediated signal transduction. Therefore, the present study was designed to disclose whether intrathecal ketamine could suppress SNL-induced spinal microglial activation and exert some antiallodynic effects on neuropathic pain by suppressing TLR3 activation. Behavioral results showed that intrathecal ketamine attenuated SNL-induced mechanical allodynia, as well as spinal microglial activation, in a dose-dependent manner. Furthermore, Western blot analysis displayed that ketamine application downregulated SNL-induced phosphorylated-p38 (p-p38) expression, which was specifically expressed in spinal microglia but not in astrocytes or neurons. Besides, ketamine could reverse TLR3 agonist (polyinosine-polycytidylic acid)-induced mechanical allodynia and spinal microglia activation. It was concluded that intrathecal ketamine depresses TLR3-induced spinal microglial p-p38 mitogen-activated protein kinase pathway activation after SNL, probably contributing to the antiallodynic effect of ketamine on SNL-induced neuropathic pain. SN - 1424-8638 UR - https://www.unboundmedicine.com/medline/citation/21389680/Ketamine_depresses_toll_like_receptor_3_signaling_in_spinal_microglia_in_a_rat_model_of_neuropathic_pain_ L2 - https://www.karger.com?DOI=10.1159/000324293 DB - PRIME DP - Unbound Medicine ER -