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DNA amount of X and B chromosomes in the grasshoppers Eyprepocnemis plorans and Locusta migratoria.
Cytogenet Genome Res. 2011; 134(2):120-6.CG

Abstract

We analyzed the DNA amount in X and B chromosomes of 2 XX/X0 grasshopper species (Eyprepocnemis plorans and Locusta migratoria), by means of Feulgen image analysis densitometry (FIAD), using previous estimates in L. migratoria as standard (5.89 pg). We first analyzed spermatids of 0B males and found a bimodal distribution of integrated optical densities (IODs), suggesting that one peak corresponded to +X and the other to -X spermatids. The difference between the 2 peaks corresponded to the X chromosome DNA amount, which was 1.28 pg in E. plorans and 0.80 pg in L. migratoria. In addition, the +X peak in E. plorans gave an estimate of the C-value in this species (10.39 pg). We next analyzed diplotene cells from 1B males in E. plorans and +B males in L. migratoria (a species where Bs are mitotically unstable and no integer B number can be defined for an individual) and measured B chromosome IOD relative to X chromosome IOD, within the same cell, taking advantage of the similar degree of condensation for both positively heteropycnotic chromosomes at this meiotic stage. From this proportion, we estimated the DNA amount for 3 different B chromosome variants found in individuals from 3 E. plorans Spanish populations (0.54 pg for B1 from Saladares, 0.51 pg for B2 from Salobreña and 0.64 for B24 from Torrox). Likewise, we estimated the DNA amount of the B chromosome in L. migratoria to be 0.15 pg. To automate measurements, we wrote a GPL3 licensed Python program (pyFIA). We discuss the utility of the present approach for estimating X and B chromosome DNA amount in a variety of situations, and the meaning of the DNA amount estimates for X and B chromosomes in these 2 species.

Authors+Show Affiliations

Departamento de Genética, Universidad de Granada, Granada, España.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21389690

Citation

Ruiz-Ruano, F J., et al. "DNA Amount of X and B Chromosomes in the Grasshoppers Eyprepocnemis Plorans and Locusta Migratoria." Cytogenetic and Genome Research, vol. 134, no. 2, 2011, pp. 120-6.
Ruiz-Ruano FJ, Ruiz-Estévez M, Rodríguez-Pérez J, et al. DNA amount of X and B chromosomes in the grasshoppers Eyprepocnemis plorans and Locusta migratoria. Cytogenet Genome Res. 2011;134(2):120-6.
Ruiz-Ruano, F. J., Ruiz-Estévez, M., Rodríguez-Pérez, J., López-Pino, J. L., Cabrero, J., & Camacho, J. P. (2011). DNA amount of X and B chromosomes in the grasshoppers Eyprepocnemis plorans and Locusta migratoria. Cytogenetic and Genome Research, 134(2), 120-6. https://doi.org/10.1159/000324690
Ruiz-Ruano FJ, et al. DNA Amount of X and B Chromosomes in the Grasshoppers Eyprepocnemis Plorans and Locusta Migratoria. Cytogenet Genome Res. 2011;134(2):120-6. PubMed PMID: 21389690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA amount of X and B chromosomes in the grasshoppers Eyprepocnemis plorans and Locusta migratoria. AU - Ruiz-Ruano,F J, AU - Ruiz-Estévez,M, AU - Rodríguez-Pérez,J, AU - López-Pino,J L, AU - Cabrero,J, AU - Camacho,J P M, Y1 - 2011/03/08/ PY - 2010/11/29/accepted PY - 2011/3/11/entrez PY - 2011/3/11/pubmed PY - 2011/7/9/medline SP - 120 EP - 6 JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. VL - 134 IS - 2 N2 - We analyzed the DNA amount in X and B chromosomes of 2 XX/X0 grasshopper species (Eyprepocnemis plorans and Locusta migratoria), by means of Feulgen image analysis densitometry (FIAD), using previous estimates in L. migratoria as standard (5.89 pg). We first analyzed spermatids of 0B males and found a bimodal distribution of integrated optical densities (IODs), suggesting that one peak corresponded to +X and the other to -X spermatids. The difference between the 2 peaks corresponded to the X chromosome DNA amount, which was 1.28 pg in E. plorans and 0.80 pg in L. migratoria. In addition, the +X peak in E. plorans gave an estimate of the C-value in this species (10.39 pg). We next analyzed diplotene cells from 1B males in E. plorans and +B males in L. migratoria (a species where Bs are mitotically unstable and no integer B number can be defined for an individual) and measured B chromosome IOD relative to X chromosome IOD, within the same cell, taking advantage of the similar degree of condensation for both positively heteropycnotic chromosomes at this meiotic stage. From this proportion, we estimated the DNA amount for 3 different B chromosome variants found in individuals from 3 E. plorans Spanish populations (0.54 pg for B1 from Saladares, 0.51 pg for B2 from Salobreña and 0.64 for B24 from Torrox). Likewise, we estimated the DNA amount of the B chromosome in L. migratoria to be 0.15 pg. To automate measurements, we wrote a GPL3 licensed Python program (pyFIA). We discuss the utility of the present approach for estimating X and B chromosome DNA amount in a variety of situations, and the meaning of the DNA amount estimates for X and B chromosomes in these 2 species. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/21389690/DNA_amount_of_X_and_B_chromosomes_in_the_grasshoppers_Eyprepocnemis_plorans_and_Locusta_migratoria_ L2 - https://www.karger.com?DOI=10.1159/000324690 DB - PRIME DP - Unbound Medicine ER -