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Stability of cefepime in aqueous eye drops.
Pharmazie. 2011 Jan; 66(1):17-23.P

Abstract

The aim of the studies was to determine HPLC the stability of cefepime in 1% and 5% buffered eye drops of developed formulary composition, which were stored for 30 days at the temperature of 4 degrees C and 20 degrees C, protected from light. Separation was performed on RP18 Gemini octadecylsilane column (250 mm x 4.6 mm, 5.0 microm) at a temperature of 25 degrees C. The mobile phase consisted of 0.015 M solution of sodium salt of pentane sulphonic acid brought to pH 4.0 with glacial acetic acid and 45% KOH solution and acetonitrile 94:6 w/w, with detection of 254 nm. The method was linear in the range of 12.6-125 microg/ml (R2 = 0.9996). The limit of detection (LOD) was 3 microg/ml and limit of quantification (LOQ) was 10 microg/ml. 10% degradation of cefepime in 1% and 5% buffered eye drops stored at the temperature of 4 degrees C, depending on the composition of the eye drops, occurred after 21-27 days in 1% eye drops and 18-21 days in 5% eye drops. In the eye drops, which were stored at the temperature of 20 degrees C, 10% degradation of cefepime took place on the third day of storage regardless of formulary composition of 1% and 5% drops. Cefepime stability lasting a couple of weeks in 1% and 5% solution allows extemporaneous preparation of buffered eye drops containing cefepime.

Authors+Show Affiliations

Department of Drug Form Technology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Bydgoszcz, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21391430

Citation

Kodym, A, et al. "Stability of Cefepime in Aqueous Eye Drops." Die Pharmazie, vol. 66, no. 1, 2011, pp. 17-23.
Kodym A, Pawłowska M, Rumiński JK, et al. Stability of cefepime in aqueous eye drops. Pharmazie. 2011;66(1):17-23.
Kodym, A., Pawłowska, M., Rumiński, J. K., Bartosińska, A., & Kieliba, A. (2011). Stability of cefepime in aqueous eye drops. Die Pharmazie, 66(1), 17-23.
Kodym A, et al. Stability of Cefepime in Aqueous Eye Drops. Pharmazie. 2011;66(1):17-23. PubMed PMID: 21391430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of cefepime in aqueous eye drops. AU - Kodym,A, AU - Pawłowska,M, AU - Rumiński,J K, AU - Bartosińska,A, AU - Kieliba,A, PY - 2011/3/12/entrez PY - 2011/3/12/pubmed PY - 2011/4/5/medline SP - 17 EP - 23 JF - Die Pharmazie JO - Pharmazie VL - 66 IS - 1 N2 - The aim of the studies was to determine HPLC the stability of cefepime in 1% and 5% buffered eye drops of developed formulary composition, which were stored for 30 days at the temperature of 4 degrees C and 20 degrees C, protected from light. Separation was performed on RP18 Gemini octadecylsilane column (250 mm x 4.6 mm, 5.0 microm) at a temperature of 25 degrees C. The mobile phase consisted of 0.015 M solution of sodium salt of pentane sulphonic acid brought to pH 4.0 with glacial acetic acid and 45% KOH solution and acetonitrile 94:6 w/w, with detection of 254 nm. The method was linear in the range of 12.6-125 microg/ml (R2 = 0.9996). The limit of detection (LOD) was 3 microg/ml and limit of quantification (LOQ) was 10 microg/ml. 10% degradation of cefepime in 1% and 5% buffered eye drops stored at the temperature of 4 degrees C, depending on the composition of the eye drops, occurred after 21-27 days in 1% eye drops and 18-21 days in 5% eye drops. In the eye drops, which were stored at the temperature of 20 degrees C, 10% degradation of cefepime took place on the third day of storage regardless of formulary composition of 1% and 5% drops. Cefepime stability lasting a couple of weeks in 1% and 5% solution allows extemporaneous preparation of buffered eye drops containing cefepime. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/21391430/Stability_of_cefepime_in_aqueous_eye_drops_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -