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Hepatoprotective activity of punarnavashtak kwath, an Ayurvedic formulation, against CCl4-induced hepatotoxicity in rats and on the HepG2 cell line.
Pharm Biol. 2011 Apr; 49(4):408-15.PB

Abstract

OBJECTIVE

Punarnavashtak kwath (PNK) is a classical Ayurvedic formulation, mentioned in Ayurvedic literature Bhaishajya Ratnavali, for hepatic disorders and asthma. This study investigated the hepatoprotective activity of PNK to validate the traditional use of this formulation.

MATERIALS AND METHODS

PNK was prepared in the laboratory according to the method given in Ayurvedic literature. Phytochemical screening was performed to determine the presence of phytoconstituents. Hepatoprotective activity was evaluated against CCl(4)-induced hepatotoxicity in rats and by its effect on the HepG2 cell line.

RESULTS

Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, saponins, and a bitter principle in PNK. Administration of PNK produced significant hepatoprotective effect as demonstrated by decreased levels of serum liver marker enzymes such as aspartate transaminase, serum alanine transaminase, serum alkaline phosphatase, and serum bilirubin and an increase in protein level. Thiopentone-induced sleeping time was also decreased in the PNK-treated animals compared with the CCl(4)-treated group. It also showed antioxidant activity by increase in activity of glutathione, superoxide dismutase, and catalase and by a decrease in thiobarbituric acid reactive substance level compared with the CCl(4)-treated group. Results of a histopathological study also support the hepatoprotective activity of PNK. Investigation carried out on the HepG2 cell line depicted significant increase in viability of cells exposed to PNK as compared with CCl(4)-treated cells.

DISCUSSION AND CONCLUSION

It can be concluded that PNK protects hepatocytes from CCl(4)-induced liver damages due to its antioxidant effect on hepatocytes. An in vitro study on HepG2 cell lines also supports its protective effect.

Authors+Show Affiliations

Department of Pharmacognosy, APMC College of Pharmaceutical Education and Research, Himatnagar, India. vnshah_02@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21391842

Citation

Shah, Vaishali N., et al. "Hepatoprotective Activity of Punarnavashtak Kwath, an Ayurvedic Formulation, Against CCl4-induced Hepatotoxicity in Rats and On the HepG2 Cell Line." Pharmaceutical Biology, vol. 49, no. 4, 2011, pp. 408-15.
Shah VN, Shah MB, Bhatt PA. Hepatoprotective activity of punarnavashtak kwath, an Ayurvedic formulation, against CCl4-induced hepatotoxicity in rats and on the HepG2 cell line. Pharm Biol. 2011;49(4):408-15.
Shah, V. N., Shah, M. B., & Bhatt, P. A. (2011). Hepatoprotective activity of punarnavashtak kwath, an Ayurvedic formulation, against CCl4-induced hepatotoxicity in rats and on the HepG2 cell line. Pharmaceutical Biology, 49(4), 408-15. https://doi.org/10.3109/13880209.2010.521162
Shah VN, Shah MB, Bhatt PA. Hepatoprotective Activity of Punarnavashtak Kwath, an Ayurvedic Formulation, Against CCl4-induced Hepatotoxicity in Rats and On the HepG2 Cell Line. Pharm Biol. 2011;49(4):408-15. PubMed PMID: 21391842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective activity of punarnavashtak kwath, an Ayurvedic formulation, against CCl4-induced hepatotoxicity in rats and on the HepG2 cell line. AU - Shah,Vaishali N, AU - Shah,Mamta B, AU - Bhatt,Parloop A, Y1 - 2011/03/11/ PY - 2011/3/12/entrez PY - 2011/3/12/pubmed PY - 2011/10/21/medline SP - 408 EP - 15 JF - Pharmaceutical biology JO - Pharm Biol VL - 49 IS - 4 N2 - OBJECTIVE: Punarnavashtak kwath (PNK) is a classical Ayurvedic formulation, mentioned in Ayurvedic literature Bhaishajya Ratnavali, for hepatic disorders and asthma. This study investigated the hepatoprotective activity of PNK to validate the traditional use of this formulation. MATERIALS AND METHODS: PNK was prepared in the laboratory according to the method given in Ayurvedic literature. Phytochemical screening was performed to determine the presence of phytoconstituents. Hepatoprotective activity was evaluated against CCl(4)-induced hepatotoxicity in rats and by its effect on the HepG2 cell line. RESULTS: Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, saponins, and a bitter principle in PNK. Administration of PNK produced significant hepatoprotective effect as demonstrated by decreased levels of serum liver marker enzymes such as aspartate transaminase, serum alanine transaminase, serum alkaline phosphatase, and serum bilirubin and an increase in protein level. Thiopentone-induced sleeping time was also decreased in the PNK-treated animals compared with the CCl(4)-treated group. It also showed antioxidant activity by increase in activity of glutathione, superoxide dismutase, and catalase and by a decrease in thiobarbituric acid reactive substance level compared with the CCl(4)-treated group. Results of a histopathological study also support the hepatoprotective activity of PNK. Investigation carried out on the HepG2 cell line depicted significant increase in viability of cells exposed to PNK as compared with CCl(4)-treated cells. DISCUSSION AND CONCLUSION: It can be concluded that PNK protects hepatocytes from CCl(4)-induced liver damages due to its antioxidant effect on hepatocytes. An in vitro study on HepG2 cell lines also supports its protective effect. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/21391842/Hepatoprotective_activity_of_punarnavashtak_kwath_an_Ayurvedic_formulation_against_CCl4_induced_hepatotoxicity_in_rats_and_on_the_HepG2_cell_line_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2010.521162 DB - PRIME DP - Unbound Medicine ER -