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Monitoring and characterization of oseltamivir-resistant pandemic (H1N1) 2009 virus, Japan, 2009-2010.
Emerg Infect Dis 2011; 17(3):470-9EI

Abstract

To monitor and characterize oseltamivir-resistant (OR) pandemic (H1N1) 2009 virus with the H275Y mutation, we analyzed 4,307 clinical specimens from Japan by neuraminidase (NA) sequencing or inhibition assay; 61 OR pandemic (H1N1) 2009 viruses were detected. NA inhibition assay and M2 sequencing indicated that OR pandemic (H1N1) 2009 virus was resistant to M2 inhibitors, but sensitive to zanamivir. Full-genome sequencing showed OR and oseltamivir-sensitive (OS) viruses had high sequence similarity, indicating that domestic OR virus was derived from OS pandemic (H1N1) 2009 virus. Hemagglutination inhibition test demonstrated that OR and OS pandemic (H1N1) 2009 viruses were antigenically similar to the A/California/7/2009 vaccine strain. Of 61 case-patients with OR viruses, 45 received oseltamivir as treatment, and 10 received it as prophylaxis, which suggests that most cases emerged sporadically from OS pandemic (H1N1) 2009, due to selective pressure. No evidence of sustained spread of OR pandemic (H1N1) 2009 was found in Japan; however, 2 suspected incidents of human-to-human transmission were reported.

Authors+Show Affiliations

National Institute of Infectious Diseases, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21392439

Citation

Ujike, Makoto, et al. "Monitoring and Characterization of Oseltamivir-resistant Pandemic (H1N1) 2009 Virus, Japan, 2009-2010." Emerging Infectious Diseases, vol. 17, no. 3, 2011, pp. 470-9.
Ujike M, Ejima M, Anraku A, et al. Monitoring and characterization of oseltamivir-resistant pandemic (H1N1) 2009 virus, Japan, 2009-2010. Emerging Infect Dis. 2011;17(3):470-9.
Ujike, M., Ejima, M., Anraku, A., Shimabukuro, K., Obuchi, M., Kishida, N., ... Odagiri, T. (2011). Monitoring and characterization of oseltamivir-resistant pandemic (H1N1) 2009 virus, Japan, 2009-2010. Emerging Infectious Diseases, 17(3), pp. 470-9. doi:10.3201/eid1703.101188.
Ujike M, et al. Monitoring and Characterization of Oseltamivir-resistant Pandemic (H1N1) 2009 Virus, Japan, 2009-2010. Emerging Infect Dis. 2011;17(3):470-9. PubMed PMID: 21392439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monitoring and characterization of oseltamivir-resistant pandemic (H1N1) 2009 virus, Japan, 2009-2010. AU - Ujike,Makoto, AU - Ejima,Miho, AU - Anraku,Akane, AU - Shimabukuro,Kozue, AU - Obuchi,Masatsugu, AU - Kishida,Noriko, AU - Hong,Xu, AU - Takashita,Emi, AU - Fujisaki,Seiichiro, AU - Yamashita,Kazuyo, AU - Horikawa,Hiroshi, AU - Kato,Yumiko, AU - Oguchi,Akio, AU - Fujita,Nobuyuki, AU - Tashiro,Masato, AU - Odagiri,Takato, AU - ,, PY - 2011/3/12/entrez PY - 2011/3/12/pubmed PY - 2011/7/21/medline SP - 470 EP - 9 JF - Emerging infectious diseases JO - Emerging Infect. Dis. VL - 17 IS - 3 N2 - To monitor and characterize oseltamivir-resistant (OR) pandemic (H1N1) 2009 virus with the H275Y mutation, we analyzed 4,307 clinical specimens from Japan by neuraminidase (NA) sequencing or inhibition assay; 61 OR pandemic (H1N1) 2009 viruses were detected. NA inhibition assay and M2 sequencing indicated that OR pandemic (H1N1) 2009 virus was resistant to M2 inhibitors, but sensitive to zanamivir. Full-genome sequencing showed OR and oseltamivir-sensitive (OS) viruses had high sequence similarity, indicating that domestic OR virus was derived from OS pandemic (H1N1) 2009 virus. Hemagglutination inhibition test demonstrated that OR and OS pandemic (H1N1) 2009 viruses were antigenically similar to the A/California/7/2009 vaccine strain. Of 61 case-patients with OR viruses, 45 received oseltamivir as treatment, and 10 received it as prophylaxis, which suggests that most cases emerged sporadically from OS pandemic (H1N1) 2009, due to selective pressure. No evidence of sustained spread of OR pandemic (H1N1) 2009 was found in Japan; however, 2 suspected incidents of human-to-human transmission were reported. SN - 1080-6059 UR - https://www.unboundmedicine.com/medline/citation/21392439/Monitoring_and_characterization_of_oseltamivir_resistant_pandemic__H1N1__2009_virus_Japan_2009_2010_ L2 - https://dx.doi.org/10.3201/eid1703.101188 DB - PRIME DP - Unbound Medicine ER -