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Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus in Hokkaido, northern main island of Japan: identification of sequence types 6 and 59 Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus.
Microb Drug Resist 2011; 17(2):241-50MD

Abstract

Prevalence and molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) were studied in Hokkaido, the main northern island of Japan. Among the 1,015 S. aureus isolates derived from clinical specimens of outpatients collected in 2009, methicillin resistance gene mecA was detected in 189 isolates (18.6%). The most frequent staphylococcal cassette chromosome mec (SCCmec) type in MRSA was II (83.1%), followed by IV (6.9%) and V (3.2%). MRSA with type II-SCCmec showed multiple drug resistance and harbored various toxin and virulence factor genes except for Panton-Valentine leucocidin (PVL) gene. These isolates were mostly classified into sequence type 5 (ST5) (or other STs in CC5) and coagulase genotype II and were thus genetically similar to hospital-acquired MRSA, which have been predominating in Japan (New York/Japan clone). PVL gene was detected in three MRSA strains belonging to ST6 (two strains) and ST59 (one strain), having type IVa- and Vt-SCCmec, respectively, and also in two methicillin-susceptible S. aureus ST121 and ST188. The arcA gene within the arginine catabolic mobile element (ACME) was detected in the two PVL-negative ST5 MRSA strains, which had type IIa- or V-SCCmec. The PVL gene-positive ST6 and ST59 CA-MRSA strains were susceptible to more antimicrobials and had less virulence factor genes than the PVL-negative ST5 MRSA, including the ACME-arcA-positive strains. In the present study, ST6 was identified as a lineage of PVL-positive CA-MRSA, the ACME-arcA was first detected in ST5 MRSA with type V-SCCmec, and ST59 Taiwanese CA-MRSA strain was isolated in Hokkaido for the first time. These findings suggest a potential spread of these emerging CA-MRSA clones in Japan.

Authors+Show Affiliations

Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan. kawaguchiya@sapmed.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21395449

Citation

Kawaguchiya, Mitsuyo, et al. "Molecular Characteristics of Community-acquired Methicillin-resistant Staphylococcus Aureus in Hokkaido, Northern Main Island of Japan: Identification of Sequence Types 6 and 59 Panton-Valentine Leucocidin-positive Community-acquired Methicillin-resistant Staphylococcus Aureus." Microbial Drug Resistance (Larchmont, N.Y.), vol. 17, no. 2, 2011, pp. 241-50.
Kawaguchiya M, Urushibara N, Kuwahara O, et al. Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus in Hokkaido, northern main island of Japan: identification of sequence types 6 and 59 Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus. Microb Drug Resist. 2011;17(2):241-50.
Kawaguchiya, M., Urushibara, N., Kuwahara, O., Ito, M., Mise, K., & Kobayashi, N. (2011). Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus in Hokkaido, northern main island of Japan: identification of sequence types 6 and 59 Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus. Microbial Drug Resistance (Larchmont, N.Y.), 17(2), pp. 241-50. doi:10.1089/mdr.2010.0136.
Kawaguchiya M, et al. Molecular Characteristics of Community-acquired Methicillin-resistant Staphylococcus Aureus in Hokkaido, Northern Main Island of Japan: Identification of Sequence Types 6 and 59 Panton-Valentine Leucocidin-positive Community-acquired Methicillin-resistant Staphylococcus Aureus. Microb Drug Resist. 2011;17(2):241-50. PubMed PMID: 21395449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus in Hokkaido, northern main island of Japan: identification of sequence types 6 and 59 Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus. AU - Kawaguchiya,Mitsuyo, AU - Urushibara,Noriko, AU - Kuwahara,Osamu, AU - Ito,Masahiko, AU - Mise,Keiji, AU - Kobayashi,Nobumichi, Y1 - 2011/03/13/ PY - 2011/3/15/entrez PY - 2011/3/15/pubmed PY - 2011/9/29/medline SP - 241 EP - 50 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb. Drug Resist. VL - 17 IS - 2 N2 - Prevalence and molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) were studied in Hokkaido, the main northern island of Japan. Among the 1,015 S. aureus isolates derived from clinical specimens of outpatients collected in 2009, methicillin resistance gene mecA was detected in 189 isolates (18.6%). The most frequent staphylococcal cassette chromosome mec (SCCmec) type in MRSA was II (83.1%), followed by IV (6.9%) and V (3.2%). MRSA with type II-SCCmec showed multiple drug resistance and harbored various toxin and virulence factor genes except for Panton-Valentine leucocidin (PVL) gene. These isolates were mostly classified into sequence type 5 (ST5) (or other STs in CC5) and coagulase genotype II and were thus genetically similar to hospital-acquired MRSA, which have been predominating in Japan (New York/Japan clone). PVL gene was detected in three MRSA strains belonging to ST6 (two strains) and ST59 (one strain), having type IVa- and Vt-SCCmec, respectively, and also in two methicillin-susceptible S. aureus ST121 and ST188. The arcA gene within the arginine catabolic mobile element (ACME) was detected in the two PVL-negative ST5 MRSA strains, which had type IIa- or V-SCCmec. The PVL gene-positive ST6 and ST59 CA-MRSA strains were susceptible to more antimicrobials and had less virulence factor genes than the PVL-negative ST5 MRSA, including the ACME-arcA-positive strains. In the present study, ST6 was identified as a lineage of PVL-positive CA-MRSA, the ACME-arcA was first detected in ST5 MRSA with type V-SCCmec, and ST59 Taiwanese CA-MRSA strain was isolated in Hokkaido for the first time. These findings suggest a potential spread of these emerging CA-MRSA clones in Japan. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/21395449/Molecular_characteristics_of_community_acquired_methicillin_resistant_Staphylococcus_aureus_in_Hokkaido_northern_main_island_of_Japan:_identification_of_sequence_types_6_and_59_Panton_Valentine_leucocidin_positive_community_acquired_methicillin_resistant_Staphylococcus_aureus_ L2 - https://www.liebertpub.com/doi/full/10.1089/mdr.2010.0136?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -