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Magnetic relaxation switch and colorimetric detection of thrombin using aptamer-functionalized gold-coated iron oxide nanoparticles.
Anal Chim Acta. 2011 Mar 18; 689(2):243-9.AC

Abstract

We describe a sensitive biosensing system combining magnetic relaxation switch diagnosis and colorimetric detection of human α-thrombin, based on the aptamer-protein interaction induced aggregation of Fe(3)O(4)@Au nanoparticles. To demonstrate the concept, gold-coated iron oxide nanoparticle was synthesized by iterative reduction of HAuCl(4) onto the dextran-coated Fe(3)O(4) nanoparticles. The resulting core-shell structure had a flowerlike shape with pretty narrow size distribution (referred to as "nanorose"). The two aptamers corresponding to human α-thrombin were conjugated separately to two distinct nanorose populations. Once a solution containing human α-thrombin was introduced, the nanoroses switched from a well dispersed state to an aggregated one, leading to a change in the spin-spin relaxation time (T(2)) as well as the UV-Vis absorption spectra of the solution. Thus the qualitative and quantitative detection method for human α-thrombin was established. The dual-mode detection is clearly advantageous in obtaining a more reliable result; the detection range is widened as well. By using the dual-mode detection method, a detectable T(2) change is observed with 1.0 nM human α-thrombin, and the detection range is from 1.6 nM to 30.4 nM.

Authors+Show Affiliations

Department of Chemistry, Institute of Biomedical Sciences, Fudan University, Shanghai, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21397080

Citation

Liang, Guohai, et al. "Magnetic Relaxation Switch and Colorimetric Detection of Thrombin Using Aptamer-functionalized Gold-coated Iron Oxide Nanoparticles." Analytica Chimica Acta, vol. 689, no. 2, 2011, pp. 243-9.
Liang G, Cai S, Zhang P, et al. Magnetic relaxation switch and colorimetric detection of thrombin using aptamer-functionalized gold-coated iron oxide nanoparticles. Anal Chim Acta. 2011;689(2):243-9.
Liang, G., Cai, S., Zhang, P., Peng, Y., Chen, H., Zhang, S., & Kong, J. (2011). Magnetic relaxation switch and colorimetric detection of thrombin using aptamer-functionalized gold-coated iron oxide nanoparticles. Analytica Chimica Acta, 689(2), 243-9. https://doi.org/10.1016/j.aca.2011.01.046
Liang G, et al. Magnetic Relaxation Switch and Colorimetric Detection of Thrombin Using Aptamer-functionalized Gold-coated Iron Oxide Nanoparticles. Anal Chim Acta. 2011 Mar 18;689(2):243-9. PubMed PMID: 21397080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnetic relaxation switch and colorimetric detection of thrombin using aptamer-functionalized gold-coated iron oxide nanoparticles. AU - Liang,Guohai, AU - Cai,Shaoyu, AU - Zhang,Peng, AU - Peng,Youyuan, AU - Chen,Hui, AU - Zhang,Song, AU - Kong,Jilie, Y1 - 2011/01/27/ PY - 2010/11/05/received PY - 2011/01/16/revised PY - 2011/01/20/accepted PY - 2011/3/15/entrez PY - 2011/3/15/pubmed PY - 2011/7/2/medline SP - 243 EP - 9 JF - Analytica chimica acta JO - Anal Chim Acta VL - 689 IS - 2 N2 - We describe a sensitive biosensing system combining magnetic relaxation switch diagnosis and colorimetric detection of human α-thrombin, based on the aptamer-protein interaction induced aggregation of Fe(3)O(4)@Au nanoparticles. To demonstrate the concept, gold-coated iron oxide nanoparticle was synthesized by iterative reduction of HAuCl(4) onto the dextran-coated Fe(3)O(4) nanoparticles. The resulting core-shell structure had a flowerlike shape with pretty narrow size distribution (referred to as "nanorose"). The two aptamers corresponding to human α-thrombin were conjugated separately to two distinct nanorose populations. Once a solution containing human α-thrombin was introduced, the nanoroses switched from a well dispersed state to an aggregated one, leading to a change in the spin-spin relaxation time (T(2)) as well as the UV-Vis absorption spectra of the solution. Thus the qualitative and quantitative detection method for human α-thrombin was established. The dual-mode detection is clearly advantageous in obtaining a more reliable result; the detection range is widened as well. By using the dual-mode detection method, a detectable T(2) change is observed with 1.0 nM human α-thrombin, and the detection range is from 1.6 nM to 30.4 nM. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/21397080/Magnetic_relaxation_switch_and_colorimetric_detection_of_thrombin_using_aptamer_functionalized_gold_coated_iron_oxide_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(11)00134-6 DB - PRIME DP - Unbound Medicine ER -