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Changes in chemical composition of cortical bone associated with bone fragility in rat model with chronic kidney disease.

Abstract

Bone fragility is a complication of chronic kidney disease (CKD). Patients on dialysis have higher risk of fracture than the general population, but the reason remains obscure. Bone strength is determined by bone mass and bone quality. Although factors affecting bone quality include microarchitecture, remodeling activity, mineral content, and collagen composition, it remains unclear which factor is critically important for bone strength in CKD. We conducted an in vivo study to elucidate the factors that reduce bone mechanical property in CKD. Rats underwent thyroparathyroidectomy and progressive partial nephrectomy (TPTx-Nx). Bone mechanical property, bone mineral density (BMD), and cortical bone chemical composition (all in femur) as well as histomorphometry (in tibia) were determined. The storage modulus, which is a mechanical factor, was reduced in CKD model rats compared with controls that underwent thyroparathyroidectomy alone (TPTx). There were no differences in BMD and histomorphometric parameters between groups. However, cortical bone chemical composition differed: mineral to matrix ratio and carbonate substitution increased whereas crystallinity decreased in TPTx-Nx. In addition, enzymatic crosslinks ratio and pentosidine to matrix ratio also increased. These changes were significant in TPTx-Nx rats with most impaired renal function. Stepwise multiple regression analysis identified mature to immature crosslink ratio and crystallinity as independent contributors to storage modulus. Deteriorated bone mechanical properties in CKD may be caused by changes in chemical composition of the cortical bone, and is independent of BMD or cancellous bone microarchitecture.

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  • Authors+Show Affiliations

    ,

    Department of Health Sciences, Oita University of Nursing and Health Sciences, Oita, 870-1201, Japan. iwasaki@oita-nhs.ac.jp

    , ,

    Source

    Bone 48:6 2011 Jun 01 pg 1260-7

    MeSH

    Absorptiometry, Photon
    Animals
    Bone Density
    Bone and Bones
    Disease Models, Animal
    Kidney Failure, Chronic
    Kidney Function Tests
    Male
    Rats
    Rats, Sprague-Dawley
    Spectrum Analysis, Raman

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21397740

    Citation

    Iwasaki, Yoshiko, et al. "Changes in Chemical Composition of Cortical Bone Associated With Bone Fragility in Rat Model With Chronic Kidney Disease." Bone, vol. 48, no. 6, 2011, pp. 1260-7.
    Iwasaki Y, Kazama JJ, Yamato H, et al. Changes in chemical composition of cortical bone associated with bone fragility in rat model with chronic kidney disease. Bone. 2011;48(6):1260-7.
    Iwasaki, Y., Kazama, J. J., Yamato, H., & Fukagawa, M. (2011). Changes in chemical composition of cortical bone associated with bone fragility in rat model with chronic kidney disease. Bone, 48(6), pp. 1260-7. doi:10.1016/j.bone.2011.03.672.
    Iwasaki Y, et al. Changes in Chemical Composition of Cortical Bone Associated With Bone Fragility in Rat Model With Chronic Kidney Disease. Bone. 2011 Jun 1;48(6):1260-7. PubMed PMID: 21397740.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Changes in chemical composition of cortical bone associated with bone fragility in rat model with chronic kidney disease. AU - Iwasaki,Yoshiko, AU - Kazama,Junichiro James, AU - Yamato,Hideyuki, AU - Fukagawa,Masafumi, Y1 - 2011/03/21/ PY - 2010/09/18/received PY - 2011/02/10/revised PY - 2011/03/04/accepted PY - 2011/3/15/entrez PY - 2011/3/15/pubmed PY - 2011/9/29/medline SP - 1260 EP - 7 JF - Bone JO - Bone VL - 48 IS - 6 N2 - Bone fragility is a complication of chronic kidney disease (CKD). Patients on dialysis have higher risk of fracture than the general population, but the reason remains obscure. Bone strength is determined by bone mass and bone quality. Although factors affecting bone quality include microarchitecture, remodeling activity, mineral content, and collagen composition, it remains unclear which factor is critically important for bone strength in CKD. We conducted an in vivo study to elucidate the factors that reduce bone mechanical property in CKD. Rats underwent thyroparathyroidectomy and progressive partial nephrectomy (TPTx-Nx). Bone mechanical property, bone mineral density (BMD), and cortical bone chemical composition (all in femur) as well as histomorphometry (in tibia) were determined. The storage modulus, which is a mechanical factor, was reduced in CKD model rats compared with controls that underwent thyroparathyroidectomy alone (TPTx). There were no differences in BMD and histomorphometric parameters between groups. However, cortical bone chemical composition differed: mineral to matrix ratio and carbonate substitution increased whereas crystallinity decreased in TPTx-Nx. In addition, enzymatic crosslinks ratio and pentosidine to matrix ratio also increased. These changes were significant in TPTx-Nx rats with most impaired renal function. Stepwise multiple regression analysis identified mature to immature crosslink ratio and crystallinity as independent contributors to storage modulus. Deteriorated bone mechanical properties in CKD may be caused by changes in chemical composition of the cortical bone, and is independent of BMD or cancellous bone microarchitecture. SN - 1873-2763 UR - https://www.unboundmedicine.com/medline/citation/21397740/Changes_in_chemical_composition_of_cortical_bone_associated_with_bone_fragility_in_rat_model_with_chronic_kidney_disease L2 - https://linkinghub.elsevier.com/retrieve/pii/S8756-3282(11)00774-5 DB - PRIME DP - Unbound Medicine ER -