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Molecular cytogenetic characterization of two high protein wheat-Thinopyrum intermedium partial amphiploids.
J Appl Genet. 2011 Aug; 52(3):269-77.JA

Abstract

Fluorescence and genomic in situ hybridization (FISH and GISH) were used to establish the cytogenetic constitution of two wheat × Thinopyrum intermedium partial amphiploids H95 and 55(1-57). Both partial amphiploids are high-protein lines having resistance to leaf rust, yellow rust and powdery mildew and have in total 56 chromosomes per cell. Repetitive DNA probes (pTa71, Afa family and pSc119.2) were used to identify the individual wheat chromosomes and to reveal the distribution of these probes within the alien chromosomes. FISH detected 6B tetrasomy in H95 and a null (1D)-tetrasomy (1B) in 55(1-57). GISH was carried out using biotin labeled Th. intermedium DNA and digoxigenin labeled Pseudoroegneria spicata DNA as probes, subsequently. GISH results revealed 44 wheat chromosomes and four Thinopyrum chromosome pairs, including three S and one J chromosome pairs in line H95. Line 55(1-57), contained 42 wheat chromosomes and six Th. intermedium pairs, including two S and one J(S) pairs. Additionally, two identical translocated chromosome pairs with diminished affinity to the alien chromatin were detected in both amphiploids. Another two translocations were found in 55(1-57), with satellite sections from the Thinopyrum J genome.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Georgieva M
Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Sepsi A
No affiliation info available
Tyankova N
No affiliation info available
Molnár-Láng M
No affiliation info available

MeSH

AscomycotaChromosome AberrationsChromosomes, PlantDNA ProbesGenome, PlantIn Situ Hybridization, FluorescenceKaryotypingMitosisPlant DiseasesPlant ProteinsTetrasomyTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21404041

Citation

Georgieva, Mariyana, et al. "Molecular Cytogenetic Characterization of Two High Protein wheat-Thinopyrum Intermedium Partial Amphiploids." Journal of Applied Genetics, vol. 52, no. 3, 2011, pp. 269-77.
Georgieva M, Sepsi A, Tyankova N, et al. Molecular cytogenetic characterization of two high protein wheat-Thinopyrum intermedium partial amphiploids. J Appl Genet. 2011;52(3):269-77.
Georgieva, M., Sepsi, A., Tyankova, N., & Molnár-Láng, M. (2011). Molecular cytogenetic characterization of two high protein wheat-Thinopyrum intermedium partial amphiploids. Journal of Applied Genetics, 52(3), 269-77. https://doi.org/10.1007/s13353-011-0037-1
Georgieva M, et al. Molecular Cytogenetic Characterization of Two High Protein wheat-Thinopyrum Intermedium Partial Amphiploids. J Appl Genet. 2011;52(3):269-77. PubMed PMID: 21404041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic characterization of two high protein wheat-Thinopyrum intermedium partial amphiploids. AU - Georgieva,Mariyana, AU - Sepsi,Adél, AU - Tyankova,Nedyalka, AU - Molnár-Láng,Márta, Y1 - 2011/03/15/ PY - 2010/11/09/received PY - 2011/02/26/accepted PY - 2011/3/16/entrez PY - 2011/3/16/pubmed PY - 2011/12/13/medline SP - 269 EP - 77 JF - Journal of applied genetics JO - J Appl Genet VL - 52 IS - 3 N2 - Fluorescence and genomic in situ hybridization (FISH and GISH) were used to establish the cytogenetic constitution of two wheat × Thinopyrum intermedium partial amphiploids H95 and 55(1-57). Both partial amphiploids are high-protein lines having resistance to leaf rust, yellow rust and powdery mildew and have in total 56 chromosomes per cell. Repetitive DNA probes (pTa71, Afa family and pSc119.2) were used to identify the individual wheat chromosomes and to reveal the distribution of these probes within the alien chromosomes. FISH detected 6B tetrasomy in H95 and a null (1D)-tetrasomy (1B) in 55(1-57). GISH was carried out using biotin labeled Th. intermedium DNA and digoxigenin labeled Pseudoroegneria spicata DNA as probes, subsequently. GISH results revealed 44 wheat chromosomes and four Thinopyrum chromosome pairs, including three S and one J chromosome pairs in line H95. Line 55(1-57), contained 42 wheat chromosomes and six Th. intermedium pairs, including two S and one J(S) pairs. Additionally, two identical translocated chromosome pairs with diminished affinity to the alien chromatin were detected in both amphiploids. Another two translocations were found in 55(1-57), with satellite sections from the Thinopyrum J genome. SN - 2190-3883 UR - https://www.unboundmedicine.com/medline/citation/21404041/Molecular_cytogenetic_characterization_of_two_high_protein_wheat_Thinopyrum_intermedium_partial_amphiploids_ L2 - https://dx.doi.org/10.1007/s13353-011-0037-1 DB - PRIME DP - Unbound Medicine ER -