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Platelet-derived growth factor receptor alpha is associated with oxidative stress-induced retinal cell death.
Curr Eye Res 2011; 36(4):336-40CE

Abstract

PURPOSE

The purpose of this study was to investigate the role played by platelet-derived growth factor-α (PDGFRα) in oxidative stress-induced retinal cell death. A previous proteomic study from our laboratory showed that expression of PDGFRα is elevated considerably in the retinas of an animal model of glaucoma-the excitatory amino acid carrier (EAAC) 1-deficient (EAAC1-/-) mouse.

METHODS

Retinal sites and expression patterns of PDGFRα were determined by immunohistochemistry in the retinas of EAAC1-/- and control CRL:CD1(ICR) mice. A retinal cell line was exposed to hydrogen peroxide, and expression PDGFRα determined by Western blot analysis. Effects of PDGF-AA and PDGFRα-siRNA on hydrogen peroxide-induced retinal cell death were examined.

RESULTS

PDGFRα was detected in the retinal ganglion cell layer (RGL) of both EAAC1-/- and ICR mice, and was also localized in the internal nuclear layer (INL) of EAAC1-/- mice. While treatment with excess PDGF-AA had no additional effect on retinal cell death, expression of PDGFRα increased with exposure to hydrogen peroxide. Hydrogen peroxide-induced retinal cell death was inhibited by exposure to PDGF-AA via phosphatidylinositol 3 kinase (PI3K); cell death was promoted by PDGFRα-siRNA.

CONCLUSIONS

PDGFRα is expressed in mouse retina, where it is essential for retinal cell survival under conditions of oxidative stress.

Authors+Show Affiliations

Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. tkana@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21405954

Citation

Kanamoto, Takashi, et al. "Platelet-derived Growth Factor Receptor Alpha Is Associated With Oxidative Stress-induced Retinal Cell Death." Current Eye Research, vol. 36, no. 4, 2011, pp. 336-40.
Kanamoto T, Rimayanti U, H O, et al. Platelet-derived growth factor receptor alpha is associated with oxidative stress-induced retinal cell death. Curr Eye Res. 2011;36(4):336-40.
Kanamoto, T., Rimayanti, U., H, O., & Kiuchi, Y. (2011). Platelet-derived growth factor receptor alpha is associated with oxidative stress-induced retinal cell death. Current Eye Research, 36(4), pp. 336-40. doi:10.3109/02713683.2011.556301.
Kanamoto T, et al. Platelet-derived Growth Factor Receptor Alpha Is Associated With Oxidative Stress-induced Retinal Cell Death. Curr Eye Res. 2011;36(4):336-40. PubMed PMID: 21405954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platelet-derived growth factor receptor alpha is associated with oxidative stress-induced retinal cell death. AU - Kanamoto,Takashi, AU - Rimayanti,Ulfah, AU - H,Okumichi, AU - Kiuchi,Yoshiaki, PY - 2011/3/17/entrez PY - 2011/3/17/pubmed PY - 2011/6/30/medline SP - 336 EP - 40 JF - Current eye research JO - Curr. Eye Res. VL - 36 IS - 4 N2 - PURPOSE: The purpose of this study was to investigate the role played by platelet-derived growth factor-α (PDGFRα) in oxidative stress-induced retinal cell death. A previous proteomic study from our laboratory showed that expression of PDGFRα is elevated considerably in the retinas of an animal model of glaucoma-the excitatory amino acid carrier (EAAC) 1-deficient (EAAC1-/-) mouse. METHODS: Retinal sites and expression patterns of PDGFRα were determined by immunohistochemistry in the retinas of EAAC1-/- and control CRL:CD1(ICR) mice. A retinal cell line was exposed to hydrogen peroxide, and expression PDGFRα determined by Western blot analysis. Effects of PDGF-AA and PDGFRα-siRNA on hydrogen peroxide-induced retinal cell death were examined. RESULTS: PDGFRα was detected in the retinal ganglion cell layer (RGL) of both EAAC1-/- and ICR mice, and was also localized in the internal nuclear layer (INL) of EAAC1-/- mice. While treatment with excess PDGF-AA had no additional effect on retinal cell death, expression of PDGFRα increased with exposure to hydrogen peroxide. Hydrogen peroxide-induced retinal cell death was inhibited by exposure to PDGF-AA via phosphatidylinositol 3 kinase (PI3K); cell death was promoted by PDGFRα-siRNA. CONCLUSIONS: PDGFRα is expressed in mouse retina, where it is essential for retinal cell survival under conditions of oxidative stress. SN - 1460-2202 UR - https://www.unboundmedicine.com/medline/citation/21405954/Platelet-derived_growth_factor_receptor_alpha_is_associated_with_oxidative_stress-induced_retinal_cell_death L2 - http://www.tandfonline.com/doi/full/10.3109/02713683.2011.556301 DB - PRIME DP - Unbound Medicine ER -