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Intravenous immunoglobulin modulates the maturation of TLR 4-primed peripheral blood monocytes.
Clin Immunol. 2011 May; 139(2):208-14.CI

Abstract

Intravenous immunoglobulin (IgIV) has immune modulating effects on the differentiation and function of dendritic cells (DC). Peripheral blood CD14+ monocytes were induced to differentiate into immature DC with IL-4/GM-CSF. DC maturation was analyzed by flow cytometry, and function assessed for antigen uptake and antigen processing. IgIV added during the differentiation process induced immature DC to differentiate into a mature DC with increased expression of CD83 and CCR7. A "priming" step with low concentrations of LPS or other TLR agonists that utilize the myD88 signaling pathway was necessary to observe these changes. These modulated DCs had reduced antigen uptake, but exhibited increased antigen presentation. Treatment of the IgIV with pepsin to generate F(ab')2 fragments abrogated these effects on DC maturation and function. The enhanced differentiation of PBM into DC required two signals: an initial exposure to low concentrations of LPS followed by IVIG. The second signal with IVIG was Fc dependent.

Authors+Show Affiliations

Allergy/Clinical Immunology Division, Women and Children's Hospital, 219 Bryant St, Buffalo, NY 14222, USA. ballow@buffalo.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21406333

Citation

Ballow, Mark, and Cheryl Allen. "Intravenous Immunoglobulin Modulates the Maturation of TLR 4-primed Peripheral Blood Monocytes." Clinical Immunology (Orlando, Fla.), vol. 139, no. 2, 2011, pp. 208-14.
Ballow M, Allen C. Intravenous immunoglobulin modulates the maturation of TLR 4-primed peripheral blood monocytes. Clin Immunol. 2011;139(2):208-14.
Ballow, M., & Allen, C. (2011). Intravenous immunoglobulin modulates the maturation of TLR 4-primed peripheral blood monocytes. Clinical Immunology (Orlando, Fla.), 139(2), 208-14. https://doi.org/10.1016/j.clim.2011.02.006
Ballow M, Allen C. Intravenous Immunoglobulin Modulates the Maturation of TLR 4-primed Peripheral Blood Monocytes. Clin Immunol. 2011;139(2):208-14. PubMed PMID: 21406333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intravenous immunoglobulin modulates the maturation of TLR 4-primed peripheral blood monocytes. AU - Ballow,Mark, AU - Allen,Cheryl, Y1 - 2011/03/15/ PY - 2010/08/25/received PY - 2011/01/28/revised PY - 2011/02/02/accepted PY - 2011/3/17/entrez PY - 2011/3/17/pubmed PY - 2011/6/24/medline SP - 208 EP - 14 JF - Clinical immunology (Orlando, Fla.) JO - Clin Immunol VL - 139 IS - 2 N2 - Intravenous immunoglobulin (IgIV) has immune modulating effects on the differentiation and function of dendritic cells (DC). Peripheral blood CD14+ monocytes were induced to differentiate into immature DC with IL-4/GM-CSF. DC maturation was analyzed by flow cytometry, and function assessed for antigen uptake and antigen processing. IgIV added during the differentiation process induced immature DC to differentiate into a mature DC with increased expression of CD83 and CCR7. A "priming" step with low concentrations of LPS or other TLR agonists that utilize the myD88 signaling pathway was necessary to observe these changes. These modulated DCs had reduced antigen uptake, but exhibited increased antigen presentation. Treatment of the IgIV with pepsin to generate F(ab')2 fragments abrogated these effects on DC maturation and function. The enhanced differentiation of PBM into DC required two signals: an initial exposure to low concentrations of LPS followed by IVIG. The second signal with IVIG was Fc dependent. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/21406333/Intravenous_immunoglobulin_modulates_the_maturation_of_TLR_4_primed_peripheral_blood_monocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(11)00055-6 DB - PRIME DP - Unbound Medicine ER -