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Matrine inhibits matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells.
J Asian Nat Prod Res. 2011 Mar; 13(3):242-50.JA

Abstract

Matrine is the major active component of the traditional Chinese medicine Sophora flavescens, but the molecular mechanisms of matrine on tumor invasion inhibition remain unclear. The aim of this study is to elucidate the effects of matrine on invasion ability of human hepatocellular carcinoma (HCC) cells, matrix metalloproteinase-9 (MMP-9), and nuclear factor (NF)-kappa B expression. The expression activity of MMP-9 was measured by reverse transcription polymerase chain reaction, Western blot, and gelatin zymography analysis. The expression of NF-kappa B was measured by the Western blot analysis. Matrine significantly inhibited MMP-9 expression of SMMC-7721 cells. NF-kappa B inhibitor PTDC induced a marked reduction in MMP-9 expression, and it suggested that NF-kappa B could play an important role in MMP-9 expression. Furthermore, matrine significantly suppressed NF-kappa B expression and the invasion of SMMC-7721 cells. Our results showed that matrine inhibited MMP-9 expression and the invasion of human HCC cells. The inhibitory effects are partly associated with the downregulation of the NF-kappa B signaling pathway.

Authors+Show Affiliations

Department of Hepatobiliary Surgery of Henan Provincial People's Hospital, Zhengzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21409686

Citation

Yu, Hai-Bo, et al. "Matrine Inhibits Matrix Metalloproteinase-9 Expression and Invasion of Human Hepatocellular Carcinoma Cells." Journal of Asian Natural Products Research, vol. 13, no. 3, 2011, pp. 242-50.
Yu HB, Zhang HF, Li DY, et al. Matrine inhibits matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells. J Asian Nat Prod Res. 2011;13(3):242-50.
Yu, H. B., Zhang, H. F., Li, D. Y., Zhang, X., Xue, H. Z., & Zhao, S. H. (2011). Matrine inhibits matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells. Journal of Asian Natural Products Research, 13(3), 242-50. https://doi.org/10.1080/10286020.2010.551641
Yu HB, et al. Matrine Inhibits Matrix Metalloproteinase-9 Expression and Invasion of Human Hepatocellular Carcinoma Cells. J Asian Nat Prod Res. 2011;13(3):242-50. PubMed PMID: 21409686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrine inhibits matrix metalloproteinase-9 expression and invasion of human hepatocellular carcinoma cells. AU - Yu,Hai-Bo, AU - Zhang,Hui-Feng, AU - Li,De-Yu, AU - Zhang,Xiao, AU - Xue,Huan-Zhou, AU - Zhao,Si-Hai, PY - 2011/3/17/entrez PY - 2011/3/17/pubmed PY - 2011/5/19/medline SP - 242 EP - 50 JF - Journal of Asian natural products research JO - J Asian Nat Prod Res VL - 13 IS - 3 N2 - Matrine is the major active component of the traditional Chinese medicine Sophora flavescens, but the molecular mechanisms of matrine on tumor invasion inhibition remain unclear. The aim of this study is to elucidate the effects of matrine on invasion ability of human hepatocellular carcinoma (HCC) cells, matrix metalloproteinase-9 (MMP-9), and nuclear factor (NF)-kappa B expression. The expression activity of MMP-9 was measured by reverse transcription polymerase chain reaction, Western blot, and gelatin zymography analysis. The expression of NF-kappa B was measured by the Western blot analysis. Matrine significantly inhibited MMP-9 expression of SMMC-7721 cells. NF-kappa B inhibitor PTDC induced a marked reduction in MMP-9 expression, and it suggested that NF-kappa B could play an important role in MMP-9 expression. Furthermore, matrine significantly suppressed NF-kappa B expression and the invasion of SMMC-7721 cells. Our results showed that matrine inhibited MMP-9 expression and the invasion of human HCC cells. The inhibitory effects are partly associated with the downregulation of the NF-kappa B signaling pathway. SN - 1477-2213 UR - https://www.unboundmedicine.com/medline/citation/21409686/Matrine_inhibits_matrix_metalloproteinase_9_expression_and_invasion_of_human_hepatocellular_carcinoma_cells_ L2 - https://www.tandfonline.com/doi/full/10.1080/10286020.2010.551641 DB - PRIME DP - Unbound Medicine ER -