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Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of dextromethorphan in human plasma samples.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 01; 879(11-12):777-82.JC

Abstract

In this paper, a novel method is described for automated determination of dextromethorphan in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and dextromethorphan as template molecule. These imprinted polymers were used as solid-phase extraction sorbent for the extraction of dextromethorphan from human plasma samples. Various parameters affecting the extraction efficiency of the MIP cartridges were evaluated. The high selectivity of the sorbent coupled to the high performance liquid chromatographic system permitted a simple and rapid analysis of this drug in plasma samples with limits of detection (LOD) and quantification (LOQ) of 0.12 ng/mL and 0.35 ng/mL, respectively. The MIP selectivity was evaluated by analyzing of the dextromethorphan in presence of several substances with similar molecular structures and properties. Results from the HPLC analyses showed that the recoveries of dextromethorphan using MIP cartridges from human plasma samples in the range of 1-50 ng/mL were higher than 87%.

Authors+Show Affiliations

Department of Chemistry, Amirkabir University of Technology, Hafez, Tehran, Iran.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21411381

Citation

Moein, Mohammad Mahdi, et al. "Molecularly Imprinted Polymer Cartridges Coupled On-line With High Performance Liquid Chromatography for Simple and Rapid Analysis of Dextromethorphan in Human Plasma Samples." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 879, no. 11-12, 2011, pp. 777-82.
Moein MM, Javanbakht M, Akbari-Adergani B. Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of dextromethorphan in human plasma samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879(11-12):777-82.
Moein, M. M., Javanbakht, M., & Akbari-Adergani, B. (2011). Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of dextromethorphan in human plasma samples. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 879(11-12), 777-82. https://doi.org/10.1016/j.jchromb.2011.02.031
Moein MM, Javanbakht M, Akbari-Adergani B. Molecularly Imprinted Polymer Cartridges Coupled On-line With High Performance Liquid Chromatography for Simple and Rapid Analysis of Dextromethorphan in Human Plasma Samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 1;879(11-12):777-82. PubMed PMID: 21411381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted polymer cartridges coupled on-line with high performance liquid chromatography for simple and rapid analysis of dextromethorphan in human plasma samples. AU - Moein,Mohammad Mahdi, AU - Javanbakht,Mehran, AU - Akbari-Adergani,Behrouz, Y1 - 2011/02/25/ PY - 2010/12/16/received PY - 2011/02/18/revised PY - 2011/02/19/accepted PY - 2011/3/18/entrez PY - 2011/3/18/pubmed PY - 2011/7/22/medline SP - 777 EP - 82 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 879 IS - 11-12 N2 - In this paper, a novel method is described for automated determination of dextromethorphan in biological fluids using molecularly imprinted solid-phase extraction (MISPE) as a sample clean-up technique combined with high performance liquid chromatography (HPLC). The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, chloroform as porogen and dextromethorphan as template molecule. These imprinted polymers were used as solid-phase extraction sorbent for the extraction of dextromethorphan from human plasma samples. Various parameters affecting the extraction efficiency of the MIP cartridges were evaluated. The high selectivity of the sorbent coupled to the high performance liquid chromatographic system permitted a simple and rapid analysis of this drug in plasma samples with limits of detection (LOD) and quantification (LOQ) of 0.12 ng/mL and 0.35 ng/mL, respectively. The MIP selectivity was evaluated by analyzing of the dextromethorphan in presence of several substances with similar molecular structures and properties. Results from the HPLC analyses showed that the recoveries of dextromethorphan using MIP cartridges from human plasma samples in the range of 1-50 ng/mL were higher than 87%. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/21411381/Molecularly_imprinted_polymer_cartridges_coupled_on_line_with_high_performance_liquid_chromatography_for_simple_and_rapid_analysis_of_dextromethorphan_in_human_plasma_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(11)00125-5 DB - PRIME DP - Unbound Medicine ER -