Tags

Type your tag names separated by a space and hit enter

A validated method for the detection and quantitation of 50 drugs of abuse and medicinal drugs in oral fluid by gas chromatography-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15; 879(13-14):859-70.JC

Abstract

Oral fluid is of increasing interest as an alternative sample matrix in drugs of abuse analysis. Compared to the conventional sample matrix blood, sample volumes of oral fluid are smaller and the concentrations of some drugs can be much lower. This imposes some restrictions on the analysis method, which has to be able to detect and quantify multiple analytes from a small sample volume at low concentrations. A sensitive multi-component method for quantitative determination of 50 drug compounds from oral fluid samples collected with the StatSure SalivaSampler™ device was developed. The compounds analysed include cannabis, cocaine, amphetamines, opioids, benzodiazepines and other psychoactive medicines. Both liquid-liquid-extraction (LLE) and solid-phase-extraction (SPE) are employed in the sample pre-treatment and the samples are analysed using gas chromatography-mass spectrometry (GC-MS) with the mass selective detector (MSD) operating in either electron ionization (EI) or negative-ion chemical ionization (NICI) mode. The method was fully validated, and the validated parameters included linearity, selectivity, accuracy, precision, and extraction efficiency. Stability of the collected samples during storage at -18°C was also studied, and even after over a year's storage all analyte concentrations were more than 60% of the original concentrations. The described method is suitable for routine analysis of oral fluid samples and it has been applied to analysis of more than 4000 oral fluid samples collected anonymously from volunteer road users in Finland during 2007-2009 as a part of the EU project DRUID (Driving under the Influence of Drugs, Alcohol and Medicines). At the moment the developed method is the most comprehensive validated analysis method for oral fluid samples.

Authors+Show Affiliations

National Institute for Health and Welfare, Helsinki, Finland. kaarina.langel@thl.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21411382

Citation

Langel, Kaarina, et al. "A Validated Method for the Detection and Quantitation of 50 Drugs of Abuse and Medicinal Drugs in Oral Fluid By Gas Chromatography-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 879, no. 13-14, 2011, pp. 859-70.
Langel K, Gunnar T, Ariniemi K, et al. A validated method for the detection and quantitation of 50 drugs of abuse and medicinal drugs in oral fluid by gas chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879(13-14):859-70.
Langel, K., Gunnar, T., Ariniemi, K., Rajamäki, O., & Lillsunde, P. (2011). A validated method for the detection and quantitation of 50 drugs of abuse and medicinal drugs in oral fluid by gas chromatography-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 879(13-14), 859-70. https://doi.org/10.1016/j.jchromb.2011.02.027
Langel K, et al. A Validated Method for the Detection and Quantitation of 50 Drugs of Abuse and Medicinal Drugs in Oral Fluid By Gas Chromatography-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15;879(13-14):859-70. PubMed PMID: 21411382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A validated method for the detection and quantitation of 50 drugs of abuse and medicinal drugs in oral fluid by gas chromatography-mass spectrometry. AU - Langel,Kaarina, AU - Gunnar,Teemu, AU - Ariniemi,Kari, AU - Rajamäki,Outi, AU - Lillsunde,Pirjo, Y1 - 2011/02/23/ PY - 2010/11/30/received PY - 2011/02/02/revised PY - 2011/02/15/accepted PY - 2011/3/18/entrez PY - 2011/3/18/pubmed PY - 2011/8/4/medline SP - 859 EP - 70 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 879 IS - 13-14 N2 - Oral fluid is of increasing interest as an alternative sample matrix in drugs of abuse analysis. Compared to the conventional sample matrix blood, sample volumes of oral fluid are smaller and the concentrations of some drugs can be much lower. This imposes some restrictions on the analysis method, which has to be able to detect and quantify multiple analytes from a small sample volume at low concentrations. A sensitive multi-component method for quantitative determination of 50 drug compounds from oral fluid samples collected with the StatSure SalivaSampler™ device was developed. The compounds analysed include cannabis, cocaine, amphetamines, opioids, benzodiazepines and other psychoactive medicines. Both liquid-liquid-extraction (LLE) and solid-phase-extraction (SPE) are employed in the sample pre-treatment and the samples are analysed using gas chromatography-mass spectrometry (GC-MS) with the mass selective detector (MSD) operating in either electron ionization (EI) or negative-ion chemical ionization (NICI) mode. The method was fully validated, and the validated parameters included linearity, selectivity, accuracy, precision, and extraction efficiency. Stability of the collected samples during storage at -18°C was also studied, and even after over a year's storage all analyte concentrations were more than 60% of the original concentrations. The described method is suitable for routine analysis of oral fluid samples and it has been applied to analysis of more than 4000 oral fluid samples collected anonymously from volunteer road users in Finland during 2007-2009 as a part of the EU project DRUID (Driving under the Influence of Drugs, Alcohol and Medicines). At the moment the developed method is the most comprehensive validated analysis method for oral fluid samples. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/21411382/A_validated_method_for_the_detection_and_quantitation_of_50_drugs_of_abuse_and_medicinal_drugs_in_oral_fluid_by_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(11)00110-3 DB - PRIME DP - Unbound Medicine ER -