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A single, recent origin of the accessory B chromosome of the grasshopper Eyprepocnemis plorans.
Genetics. 2011 Mar; 187(3):853-63.G

Abstract

B chromosomes are dispensable chromosomes found in >2000 eukaryotic species, usually behaving as genomic parasites. Most B chromosomes seem to be made up of the same kind of DNA sequences present in the A chromosomes. This sequence similarity makes it difficult to obtain specific molecular probes that may permit B-presence diagnosis without cytogenetic analysis. We have developed a sequence-characterized amplified region (SCAR) marker for B chromosomes in the grasshopper Eyprepocnemis plorans, which specifically amplifies a 1510-bp DNA fragment exclusively in B-carrying individuals. Fluorescent in situ hybridization and fiber FISH analyses showed that this marker is a tandemly repeated DNA sequence closely intermingled with 45S rDNA. PCR reactions showed the presence of SCAR-like sequences in the A chromosomes, but in two separate fragments, supporting the intraspecific origin of B chromosomes in this species. SCAR marker DNA sequence showed to be identical in B chromosome variants from several localities from Spain and Morocco, and it was very similar to those found in B chromosome variants from Greece and Armenia. This strongly suggests that this sequence was already present in the ancestral B chromosome of this species. In addition, the scarce sequence variation observed among several B variants from very distant populations suggests either a functional constraint or, more likely, a recent and unique origin for B chromosomes in this species.

Authors+Show Affiliations

Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21411624

Citation

Muñoz-Pajares, A Jesús, et al. "A Single, Recent Origin of the Accessory B Chromosome of the Grasshopper Eyprepocnemis Plorans." Genetics, vol. 187, no. 3, 2011, pp. 853-63.
Muñoz-Pajares AJ, Martínez-Rodríguez L, Teruel M, et al. A single, recent origin of the accessory B chromosome of the grasshopper Eyprepocnemis plorans. Genetics. 2011;187(3):853-63.
Muñoz-Pajares, A. J., Martínez-Rodríguez, L., Teruel, M., Cabrero, J., Camacho, J. P., & Perfectti, F. (2011). A single, recent origin of the accessory B chromosome of the grasshopper Eyprepocnemis plorans. Genetics, 187(3), 853-63. https://doi.org/10.1534/genetics.110.122713
Muñoz-Pajares AJ, et al. A Single, Recent Origin of the Accessory B Chromosome of the Grasshopper Eyprepocnemis Plorans. Genetics. 2011;187(3):853-63. PubMed PMID: 21411624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A single, recent origin of the accessory B chromosome of the grasshopper Eyprepocnemis plorans. AU - Muñoz-Pajares,A Jesús, AU - Martínez-Rodríguez,Laura, AU - Teruel,María, AU - Cabrero,Josefa, AU - Camacho,Juan Pedro M, AU - Perfectti,Francisco, PY - 2011/3/18/entrez PY - 2011/3/18/pubmed PY - 2011/7/13/medline SP - 853 EP - 63 JF - Genetics JO - Genetics VL - 187 IS - 3 N2 - B chromosomes are dispensable chromosomes found in >2000 eukaryotic species, usually behaving as genomic parasites. Most B chromosomes seem to be made up of the same kind of DNA sequences present in the A chromosomes. This sequence similarity makes it difficult to obtain specific molecular probes that may permit B-presence diagnosis without cytogenetic analysis. We have developed a sequence-characterized amplified region (SCAR) marker for B chromosomes in the grasshopper Eyprepocnemis plorans, which specifically amplifies a 1510-bp DNA fragment exclusively in B-carrying individuals. Fluorescent in situ hybridization and fiber FISH analyses showed that this marker is a tandemly repeated DNA sequence closely intermingled with 45S rDNA. PCR reactions showed the presence of SCAR-like sequences in the A chromosomes, but in two separate fragments, supporting the intraspecific origin of B chromosomes in this species. SCAR marker DNA sequence showed to be identical in B chromosome variants from several localities from Spain and Morocco, and it was very similar to those found in B chromosome variants from Greece and Armenia. This strongly suggests that this sequence was already present in the ancestral B chromosome of this species. In addition, the scarce sequence variation observed among several B variants from very distant populations suggests either a functional constraint or, more likely, a recent and unique origin for B chromosomes in this species. SN - 1943-2631 UR - https://www.unboundmedicine.com/medline/citation/21411624/A_single_recent_origin_of_the_accessory_B_chromosome_of_the_grasshopper_Eyprepocnemis_plorans_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=21411624 DB - PRIME DP - Unbound Medicine ER -