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Hepatitis C virus sequences from different patients confirm the existence and transmissibility of subtype 2q, a rare subtype circulating in the metropolitan area of Barcelona, Spain.
J Med Virol. 2011 May; 83(5):820-6.JM

Abstract

The hepatitis C virus (HCV) has been classified into six genotypes and more than 70 subtypes with distinct geographical and epidemiological distributions. While 18 genotype 2 subtypes have been proposed, only 5 have had their complete sequence determined. The aim of this study was to characterize HCV isolates from three patients from the Barcelona metropolitan area of Spain for whom commercial genotyping methods provided discordant results. Full-length genome sequencing was carried out for 2 of the 3 patients; for the third patient only partial NS5B sequences could be obtained. The generated sequences were subjected to phylogenetic, recombination, and identity analyses. Sequences covering most of the HCV genome (9398 and 9566 nt in length) were obtained and showed a 90.3% identity to each other at the nucleotide level, while both sequences differed by 17.5-22.6% from the other fully sequenced genotype 2 subtypes. No evidence of recombination was found. The NS5B phylogenetic tree showed that sequences from the three patients cluster together with the only representative sequence of the provisionally designed 2q subtype, which also corresponds to a patient from Barcelona. Phylogenetic analysis of the full coding sequence showed that subtype 2q was more closely related to subtype 2k. The results obtained in this study suggest that subtype 2q now meets the requirements for confirmed designation status according to consensus criteria for HCV classification and nomenclature, and its epidemiological value is ensured as it has spread among several patients in the Barcelona metropolitan area.

Authors+Show Affiliations

Microbiology Service, Health Sciences Research Institute of Germans Trias i Pujol Foundation, Germans Trias i Pujol Hospital, Autonoma University of Barcelona, Badalona, Spain. emartro.igtp.germanstrias@gencat.catNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21412791

Citation

Martró, Elisa, et al. "Hepatitis C Virus Sequences From Different Patients Confirm the Existence and Transmissibility of Subtype 2q, a Rare Subtype Circulating in the Metropolitan Area of Barcelona, Spain." Journal of Medical Virology, vol. 83, no. 5, 2011, pp. 820-6.
Martró E, Valero A, Jordana-Lluch E, et al. Hepatitis C virus sequences from different patients confirm the existence and transmissibility of subtype 2q, a rare subtype circulating in the metropolitan area of Barcelona, Spain. J Med Virol. 2011;83(5):820-6.
Martró, E., Valero, A., Jordana-Lluch, E., Saludes, V., Planas, R., González-Candelas, F., Ausina, V., & Bracho, M. A. (2011). Hepatitis C virus sequences from different patients confirm the existence and transmissibility of subtype 2q, a rare subtype circulating in the metropolitan area of Barcelona, Spain. Journal of Medical Virology, 83(5), 820-6. https://doi.org/10.1002/jmv.22054
Martró E, et al. Hepatitis C Virus Sequences From Different Patients Confirm the Existence and Transmissibility of Subtype 2q, a Rare Subtype Circulating in the Metropolitan Area of Barcelona, Spain. J Med Virol. 2011;83(5):820-6. PubMed PMID: 21412791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatitis C virus sequences from different patients confirm the existence and transmissibility of subtype 2q, a rare subtype circulating in the metropolitan area of Barcelona, Spain. AU - Martró,Elisa, AU - Valero,Ana, AU - Jordana-Lluch,Elena, AU - Saludes,Verónica, AU - Planas,Ramón, AU - González-Candelas,Fernando, AU - Ausina,Vicente, AU - Bracho,Maria Alma, PY - 2011/3/18/entrez PY - 2011/3/18/pubmed PY - 2011/7/16/medline SP - 820 EP - 6 JF - Journal of medical virology JO - J Med Virol VL - 83 IS - 5 N2 - The hepatitis C virus (HCV) has been classified into six genotypes and more than 70 subtypes with distinct geographical and epidemiological distributions. While 18 genotype 2 subtypes have been proposed, only 5 have had their complete sequence determined. The aim of this study was to characterize HCV isolates from three patients from the Barcelona metropolitan area of Spain for whom commercial genotyping methods provided discordant results. Full-length genome sequencing was carried out for 2 of the 3 patients; for the third patient only partial NS5B sequences could be obtained. The generated sequences were subjected to phylogenetic, recombination, and identity analyses. Sequences covering most of the HCV genome (9398 and 9566 nt in length) were obtained and showed a 90.3% identity to each other at the nucleotide level, while both sequences differed by 17.5-22.6% from the other fully sequenced genotype 2 subtypes. No evidence of recombination was found. The NS5B phylogenetic tree showed that sequences from the three patients cluster together with the only representative sequence of the provisionally designed 2q subtype, which also corresponds to a patient from Barcelona. Phylogenetic analysis of the full coding sequence showed that subtype 2q was more closely related to subtype 2k. The results obtained in this study suggest that subtype 2q now meets the requirements for confirmed designation status according to consensus criteria for HCV classification and nomenclature, and its epidemiological value is ensured as it has spread among several patients in the Barcelona metropolitan area. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/21412791/Hepatitis_C_virus_sequences_from_different_patients_confirm_the_existence_and_transmissibility_of_subtype_2q_a_rare_subtype_circulating_in_the_metropolitan_area_of_Barcelona_Spain_ L2 - https://doi.org/10.1002/jmv.22054 DB - PRIME DP - Unbound Medicine ER -