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Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen).
Pest Manag Sci. 2011 Jun; 67(6):697-704.PM

Abstract

BACKGROUND

Synthetic pyrethroids are the primary insecticides that are widely used for controlling Locusta migratoria manilensis (Meyen), a major pest in eastern and southern Asia and the Pacific region. In this paper, ten cDNAs encoding glutathione S-transferases (GSTs) were sequenced and characterised in L. migratoria manilensis. The effects of deltamethrin on the ten GST gene expressions were studied.

RESULTS

Phylogenetic analysis revealed nine GSTs in three different classes, including seven in sigma, one in delta and one in theta. The remaining GST (LmGSTu1) was unclassified. RT-PCR analysis showed that most GST genes were expressed in all tissues examined, including the foregut, midgut, gastric caecum, hindgut, Malpighian tubules, fat bodies, muscles, spermaries and ovaries, except that LmGSTs2, LmGSTs4, LmGSTs7 and LmGSTu1 were expressed in several tissues. LmGSTu1 appeared to be the only gene whose expressions could not be detected in eggs. Real-time quantitative PCR showed that deltamethrin at 0.08 and/or 0.12 µg mL⁻¹ increased almost all ten GST gene expressions in third-instar nymph locusts. However, deltamethrin at 0.16 and/or 0.2 µg mL⁻¹ decreased the expressions of LmGSTd1, LmGSTs1, LmGSTs5 and LmGSTs6.

CONCLUSION

The increases in GST gene expressions after deltamethrin exposure in L. migratoria manilensis might result in its elevating tolerance to other insecticides and xenobiotics.

Authors+Show Affiliations

Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, China; Research Centre of Environmental Science and Engineering, Shanxi University, Taiyuan, Shanxi, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21413139

Citation

Qin, Guohua, et al. "Identification and Characterisation of Ten Glutathione S-transferase Genes From Oriental Migratory Locust, Locusta Migratoria Manilensis (Meyen)." Pest Management Science, vol. 67, no. 6, 2011, pp. 697-704.
Qin G, Jia M, Liu T, et al. Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen). Pest Manag Sci. 2011;67(6):697-704.
Qin, G., Jia, M., Liu, T., Xuan, T., Yan Zhu, K., Guo, Y., Ma, E., & Zhang, J. (2011). Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen). Pest Management Science, 67(6), 697-704. https://doi.org/10.1002/ps.2110
Qin G, et al. Identification and Characterisation of Ten Glutathione S-transferase Genes From Oriental Migratory Locust, Locusta Migratoria Manilensis (Meyen). Pest Manag Sci. 2011;67(6):697-704. PubMed PMID: 21413139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterisation of ten glutathione S-transferase genes from oriental migratory locust, Locusta migratoria manilensis (Meyen). AU - Qin,Guohua, AU - Jia,Miao, AU - Liu,Ting, AU - Xuan,Tao, AU - Yan Zhu,Kun, AU - Guo,Yaping, AU - Ma,Enbo, AU - Zhang,Jianzhen, Y1 - 2011/03/16/ PY - 2010/04/27/received PY - 2010/09/20/revised PY - 2010/11/21/accepted PY - 2011/3/18/entrez PY - 2011/3/18/pubmed PY - 2012/9/25/medline SP - 697 EP - 704 JF - Pest management science JO - Pest Manag Sci VL - 67 IS - 6 N2 - BACKGROUND: Synthetic pyrethroids are the primary insecticides that are widely used for controlling Locusta migratoria manilensis (Meyen), a major pest in eastern and southern Asia and the Pacific region. In this paper, ten cDNAs encoding glutathione S-transferases (GSTs) were sequenced and characterised in L. migratoria manilensis. The effects of deltamethrin on the ten GST gene expressions were studied. RESULTS: Phylogenetic analysis revealed nine GSTs in three different classes, including seven in sigma, one in delta and one in theta. The remaining GST (LmGSTu1) was unclassified. RT-PCR analysis showed that most GST genes were expressed in all tissues examined, including the foregut, midgut, gastric caecum, hindgut, Malpighian tubules, fat bodies, muscles, spermaries and ovaries, except that LmGSTs2, LmGSTs4, LmGSTs7 and LmGSTu1 were expressed in several tissues. LmGSTu1 appeared to be the only gene whose expressions could not be detected in eggs. Real-time quantitative PCR showed that deltamethrin at 0.08 and/or 0.12 µg mL⁻¹ increased almost all ten GST gene expressions in third-instar nymph locusts. However, deltamethrin at 0.16 and/or 0.2 µg mL⁻¹ decreased the expressions of LmGSTd1, LmGSTs1, LmGSTs5 and LmGSTs6. CONCLUSION: The increases in GST gene expressions after deltamethrin exposure in L. migratoria manilensis might result in its elevating tolerance to other insecticides and xenobiotics. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/21413139/Identification_and_characterisation_of_ten_glutathione_S_transferase_genes_from_oriental_migratory_locust_Locusta_migratoria_manilensis__Meyen__ L2 - https://doi.org/10.1002/ps.2110 DB - PRIME DP - Unbound Medicine ER -