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Centaurium erythrea (Gentianaceae) leaf extract alleviates streptozotocin-induced oxidative stress and β-cell damage in rat pancreas.
J Ethnopharmacol. 2011 May 17; 135(2):243-50.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Centaurium erytrea is used in traditional medicine for treat urine retention, abdominal colic and diabetes mellitus. The aim of the present study was to evaluate the possible protective effects of Centaurium erythrea leaf extract (CE) against pancreas β-cells' damage and antioxidant defense systems in streptozotocin induced diabetes rats.

MATERIALS AND METHODS

Experimental diabetes was induced by a single dose of STZ (65 mg/kg) administered by intraperitoneal way. The oxidative stress was measured by tissue MDA levels, protein carbonyl (PCO) content, reduced glutathione (GSH) content and by enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in pancreas. Biochemical observations were further substantiated with histological examination of pancreas.

RESULTS

The increase in blood glucose and MDA levels with the decrease in GSH content and in enzymatic activities were the salient features observed in diabetic rats. Administration of CE (200mg/kg bw/day, i.p) for 30 days caused a significant reduction in blood glucose and MDA levels in STZ treated rats when compared with diabetic rats. Furthermore, diabetic rats treated with CE leaf extract showed a significant increase in the activities of both enzymatic and non-enzymatic antioxidants when compared to those of diabetic rats. Degenerative changes of pancreatic β-cells in STZ treated rats were minimized to near normal morphology by administration of CE leaf extract as evidenced by histopathological examination.

CONCLUSION

Results clearly indicate that Centaurium erythrea treatment exerts a therapeutic protective nature in diabetes by decreasing oxidative stress and pancreatic β-cells' damage which may be attributed to its antioxidative potential.

Authors+Show Affiliations

Animal Physiology Laboratory, UR/08-73, Sciences Faculty of Sfax, BP 1171, 3000 Sfax, University of Sfax, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21414399

Citation

Sefi, Mediha, et al. "Centaurium Erythrea (Gentianaceae) Leaf Extract Alleviates Streptozotocin-induced Oxidative Stress and Β-cell Damage in Rat Pancreas." Journal of Ethnopharmacology, vol. 135, no. 2, 2011, pp. 243-50.
Sefi M, Fetoui H, Lachkar N, et al. Centaurium erythrea (Gentianaceae) leaf extract alleviates streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. J Ethnopharmacol. 2011;135(2):243-50.
Sefi, M., Fetoui, H., Lachkar, N., Tahraoui, A., Lyoussi, B., Boudawara, T., & Zeghal, N. (2011). Centaurium erythrea (Gentianaceae) leaf extract alleviates streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. Journal of Ethnopharmacology, 135(2), 243-50. https://doi.org/10.1016/j.jep.2011.02.029
Sefi M, et al. Centaurium Erythrea (Gentianaceae) Leaf Extract Alleviates Streptozotocin-induced Oxidative Stress and Β-cell Damage in Rat Pancreas. J Ethnopharmacol. 2011 May 17;135(2):243-50. PubMed PMID: 21414399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Centaurium erythrea (Gentianaceae) leaf extract alleviates streptozotocin-induced oxidative stress and β-cell damage in rat pancreas. AU - Sefi,Mediha, AU - Fetoui,Hamadi, AU - Lachkar,Nadya, AU - Tahraoui,Adil, AU - Lyoussi,Badiaa, AU - Boudawara,Tahia, AU - Zeghal,Najiba, Y1 - 2011/03/23/ PY - 2010/05/11/received PY - 2010/12/13/revised PY - 2011/02/19/accepted PY - 2011/3/19/entrez PY - 2011/3/19/pubmed PY - 2011/9/8/medline SP - 243 EP - 50 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 135 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Centaurium erytrea is used in traditional medicine for treat urine retention, abdominal colic and diabetes mellitus. The aim of the present study was to evaluate the possible protective effects of Centaurium erythrea leaf extract (CE) against pancreas β-cells' damage and antioxidant defense systems in streptozotocin induced diabetes rats. MATERIALS AND METHODS: Experimental diabetes was induced by a single dose of STZ (65 mg/kg) administered by intraperitoneal way. The oxidative stress was measured by tissue MDA levels, protein carbonyl (PCO) content, reduced glutathione (GSH) content and by enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in pancreas. Biochemical observations were further substantiated with histological examination of pancreas. RESULTS: The increase in blood glucose and MDA levels with the decrease in GSH content and in enzymatic activities were the salient features observed in diabetic rats. Administration of CE (200mg/kg bw/day, i.p) for 30 days caused a significant reduction in blood glucose and MDA levels in STZ treated rats when compared with diabetic rats. Furthermore, diabetic rats treated with CE leaf extract showed a significant increase in the activities of both enzymatic and non-enzymatic antioxidants when compared to those of diabetic rats. Degenerative changes of pancreatic β-cells in STZ treated rats were minimized to near normal morphology by administration of CE leaf extract as evidenced by histopathological examination. CONCLUSION: Results clearly indicate that Centaurium erythrea treatment exerts a therapeutic protective nature in diabetes by decreasing oxidative stress and pancreatic β-cells' damage which may be attributed to its antioxidative potential. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21414399/Centaurium_erythrea__Gentianaceae__leaf_extract_alleviates_streptozotocin_induced_oxidative_stress_and_β_cell_damage_in_rat_pancreas_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00114-0 DB - PRIME DP - Unbound Medicine ER -