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Alloimmunity-induced autoimmunity as a potential mechanism in the pathogenesis of chronic rejection of human lung allografts.
J Heart Lung Transplant. 2011 Jun; 30(6):624-31.JH

Abstract

BACKGROUND

Bronchiolitis obliterans syndrome (BOS) is a major cause of morbidity and mortality after lung transplantation (LTx). We sought to better understand the relationship between alloimmune responses and autoimmunity and, subsequently, how autoimmunity leads to chronic rejection.

METHODS

We analyzed the development of donor-specific antibodies (Abs) in LTx by flow PRA and the development of Abs to K-α1 tubulin (K-α1T) and collagen V (ColV) by ELISA. The frequency of K-α1T- and ColV-specific T cells that secrete IFN-γ, IL-17 and IL-10 in LTx recipients was measured by ELISPOT.

RESULTS

In a retrospective analysis of 42 LTx recipients, we demonstrated a strong correlation between development of donor-specific anti-HLA Abs, Abs to self-antigens and BOS (p < 0.05). To test the hypothesis that alloimmunity is related to an immune response to self-antigens, we analyzed 103 LTx patients prospectively for the development of donor-specific Abs (DSA) and Abs to self-antigens. A total of 42.7% of recipients developed DSA and 30.1% developed Abs to K-α1T and ColV. Development of DSA preceded development of Abs to self-antigens. BOS(+) patients had higher frequency of T cells secreting IL-17 (p < 0.01) and IFN-γ (p < 0.05) with decreased IL-10 (p < 0.05) when compared with BOS(-) patients.

CONCLUSIONS

Based on these results we propose that alloimmune responses to donor HLA can induce autoimmune responses to airway epithelial self-antigens, characterized by activation of the IL-17 pathway. These immune responses to self-antigens along with alloimmunity contribute to the pathogenesis of BOS. Strategies to prevent development of autoimmunity may be play a key role in preventing the development of chronic rejection.

Authors+Show Affiliations

Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21414808

Citation

Saini, Deepti, et al. "Alloimmunity-induced Autoimmunity as a Potential Mechanism in the Pathogenesis of Chronic Rejection of Human Lung Allografts." The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation, vol. 30, no. 6, 2011, pp. 624-31.
Saini D, Weber J, Ramachandran S, et al. Alloimmunity-induced autoimmunity as a potential mechanism in the pathogenesis of chronic rejection of human lung allografts. J Heart Lung Transplant. 2011;30(6):624-31.
Saini, D., Weber, J., Ramachandran, S., Phelan, D., Tiriveedhi, V., Liu, M., Steward, N., Aloush, A., Hachem, R., Trulock, E., Meyers, B., Patterson, G. A., & Mohanakumar, T. (2011). Alloimmunity-induced autoimmunity as a potential mechanism in the pathogenesis of chronic rejection of human lung allografts. The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation, 30(6), 624-31. https://doi.org/10.1016/j.healun.2011.01.708
Saini D, et al. Alloimmunity-induced Autoimmunity as a Potential Mechanism in the Pathogenesis of Chronic Rejection of Human Lung Allografts. J Heart Lung Transplant. 2011;30(6):624-31. PubMed PMID: 21414808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alloimmunity-induced autoimmunity as a potential mechanism in the pathogenesis of chronic rejection of human lung allografts. AU - Saini,Deepti, AU - Weber,Joseph, AU - Ramachandran,Sabarinathan, AU - Phelan,Donna, AU - Tiriveedhi,Venkataswarup, AU - Liu,Michael, AU - Steward,Nancy, AU - Aloush,Aviva, AU - Hachem,Ramsey, AU - Trulock,Elbert, AU - Meyers,Bryan, AU - Patterson,G Alexander, AU - Mohanakumar,Thalachallour, Y1 - 2011/03/16/ PY - 2010/10/08/received PY - 2010/12/06/revised PY - 2011/01/17/accepted PY - 2011/3/19/entrez PY - 2011/3/19/pubmed PY - 2011/8/30/medline SP - 624 EP - 31 JF - The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation JO - J. Heart Lung Transplant. VL - 30 IS - 6 N2 - BACKGROUND: Bronchiolitis obliterans syndrome (BOS) is a major cause of morbidity and mortality after lung transplantation (LTx). We sought to better understand the relationship between alloimmune responses and autoimmunity and, subsequently, how autoimmunity leads to chronic rejection. METHODS: We analyzed the development of donor-specific antibodies (Abs) in LTx by flow PRA and the development of Abs to K-α1 tubulin (K-α1T) and collagen V (ColV) by ELISA. The frequency of K-α1T- and ColV-specific T cells that secrete IFN-γ, IL-17 and IL-10 in LTx recipients was measured by ELISPOT. RESULTS: In a retrospective analysis of 42 LTx recipients, we demonstrated a strong correlation between development of donor-specific anti-HLA Abs, Abs to self-antigens and BOS (p < 0.05). To test the hypothesis that alloimmunity is related to an immune response to self-antigens, we analyzed 103 LTx patients prospectively for the development of donor-specific Abs (DSA) and Abs to self-antigens. A total of 42.7% of recipients developed DSA and 30.1% developed Abs to K-α1T and ColV. Development of DSA preceded development of Abs to self-antigens. BOS(+) patients had higher frequency of T cells secreting IL-17 (p < 0.01) and IFN-γ (p < 0.05) with decreased IL-10 (p < 0.05) when compared with BOS(-) patients. CONCLUSIONS: Based on these results we propose that alloimmune responses to donor HLA can induce autoimmune responses to airway epithelial self-antigens, characterized by activation of the IL-17 pathway. These immune responses to self-antigens along with alloimmunity contribute to the pathogenesis of BOS. Strategies to prevent development of autoimmunity may be play a key role in preventing the development of chronic rejection. SN - 1557-3117 UR - https://www.unboundmedicine.com/medline/citation/21414808/Alloimmunity_induced_autoimmunity_as_a_potential_mechanism_in_the_pathogenesis_of_chronic_rejection_of_human_lung_allografts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1053-2498(11)00739-X DB - PRIME DP - Unbound Medicine ER -