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A comparative study of tissue ω-6 and ω-3 polyunsaturated fatty acids (PUFA) in benign and malignant pathologic stage pT2a radical prostatectomy specimens.
Urol Oncol. 2013 Apr; 31(3):318-24.UO

Abstract

OBJECTIVE

To analyze different polyunsaturated fatty acid (PUFA) tissue levels in malignant compared with benign prostatic tissue from the same prostate specimens.

MATERIALS AND METHODS

Fresh frozen benign and malignant prostatic tissue was obtained from radical prostatectomy specimens in 49 men with pathologic stage pT2a prostate cancer. Histopathologic examination confirmed that all tissues from each prostate being analyzed were either completely benign or almost totally malignant. The PUFA composition in these tissues was determined by gas-liquid chromatography on a capillary column. The relative amount of each PUFA (% of total fatty acids) was quantified by integrating the area under the peak and dividing the result by the total area of all fatty acids.

RESULTS

Tissue levels of dihomo-γ-linolenic acid, (C20:3w6), an ω-6 PUFA and a major precursor of ω-6 PUFA metabolites, were significantly higher in malignant compared with benign tissues (P = 0.002). Tissue levels of the downstream ω-6 metabolites, arachidonic acid (AA) (20:4ω6), and adrenic acid, (22:4ω6), were significantly lower in cancer tissues, (P < 0.0001 and P = 0.013, respectively). Overall, the total levels of ω-6 PUFA were lower in cancer (P = 0.001).

CONCLUSION

We found that the ω-6 PUFA AA and adrenic acid are decreased in malignant prostatic tissues compared with benign tissues from the same prostates. These findings provide additional evidence that dietary fat is associated with prostatic carcinogenesis.

Authors+Show Affiliations

Department of Molecular Medicine and Surgery, Karolinska Institutet Department of Urology, Stockholm, Sweden. Martin.Schumacher@ki.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21414816

Citation

Schumacher, Martin C., et al. "A Comparative Study of Tissue Ω-6 and Ω-3 Polyunsaturated Fatty Acids (PUFA) in Benign and Malignant Pathologic Stage pT2a Radical Prostatectomy Specimens." Urologic Oncology, vol. 31, no. 3, 2013, pp. 318-24.
Schumacher MC, Laven B, Petersson F, et al. A comparative study of tissue ω-6 and ω-3 polyunsaturated fatty acids (PUFA) in benign and malignant pathologic stage pT2a radical prostatectomy specimens. Urol Oncol. 2013;31(3):318-24.
Schumacher, M. C., Laven, B., Petersson, F., Cederholm, T., Onelöv, E., Ekman, P., & Brendler, C. (2013). A comparative study of tissue ω-6 and ω-3 polyunsaturated fatty acids (PUFA) in benign and malignant pathologic stage pT2a radical prostatectomy specimens. Urologic Oncology, 31(3), 318-24. https://doi.org/10.1016/j.urolonc.2011.01.014
Schumacher MC, et al. A Comparative Study of Tissue Ω-6 and Ω-3 Polyunsaturated Fatty Acids (PUFA) in Benign and Malignant Pathologic Stage pT2a Radical Prostatectomy Specimens. Urol Oncol. 2013;31(3):318-24. PubMed PMID: 21414816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study of tissue ω-6 and ω-3 polyunsaturated fatty acids (PUFA) in benign and malignant pathologic stage pT2a radical prostatectomy specimens. AU - Schumacher,Martin C, AU - Laven,Brett, AU - Petersson,Fredrik, AU - Cederholm,Tommy, AU - Onelöv,Eric, AU - Ekman,Peter, AU - Brendler,Charles, Y1 - 2011/04/01/ PY - 2010/10/19/received PY - 2011/01/21/revised PY - 2011/01/24/accepted PY - 2011/3/19/entrez PY - 2011/3/19/pubmed PY - 2013/9/17/medline SP - 318 EP - 24 JF - Urologic oncology JO - Urol. Oncol. VL - 31 IS - 3 N2 - OBJECTIVE: To analyze different polyunsaturated fatty acid (PUFA) tissue levels in malignant compared with benign prostatic tissue from the same prostate specimens. MATERIALS AND METHODS: Fresh frozen benign and malignant prostatic tissue was obtained from radical prostatectomy specimens in 49 men with pathologic stage pT2a prostate cancer. Histopathologic examination confirmed that all tissues from each prostate being analyzed were either completely benign or almost totally malignant. The PUFA composition in these tissues was determined by gas-liquid chromatography on a capillary column. The relative amount of each PUFA (% of total fatty acids) was quantified by integrating the area under the peak and dividing the result by the total area of all fatty acids. RESULTS: Tissue levels of dihomo-γ-linolenic acid, (C20:3w6), an ω-6 PUFA and a major precursor of ω-6 PUFA metabolites, were significantly higher in malignant compared with benign tissues (P = 0.002). Tissue levels of the downstream ω-6 metabolites, arachidonic acid (AA) (20:4ω6), and adrenic acid, (22:4ω6), were significantly lower in cancer tissues, (P < 0.0001 and P = 0.013, respectively). Overall, the total levels of ω-6 PUFA were lower in cancer (P = 0.001). CONCLUSION: We found that the ω-6 PUFA AA and adrenic acid are decreased in malignant prostatic tissues compared with benign tissues from the same prostates. These findings provide additional evidence that dietary fat is associated with prostatic carcinogenesis. SN - 1873-2496 UR - https://www.unboundmedicine.com/medline/citation/21414816/A_comparative_study_of_tissue_ω_6_and_ω_3_polyunsaturated_fatty_acids__PUFA__in_benign_and_malignant_pathologic_stage_pT2a_radical_prostatectomy_specimens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1078-1439(11)00037-8 DB - PRIME DP - Unbound Medicine ER -