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Subsets of CD3+ (T cell receptor alpha/beta or gamma/delta) and CD3- lymphocytes isolated from normal human gut epithelium display phenotypical features different from their counterparts in peripheral blood.
Eur J Immunol. 1990 May; 20(5):1097-103.EJ

Abstract

Intestinal intraepithelial lymphocytes (IEL) were studied, after isolation in humans, for their surface antigens with a large variety of monoclonal antibodies. They show peculiar characteristics when compared with peripheral blood lymphocytes and intestinal lamina propria lymphocytes. Although a majority of human intraepithelial lymphocytes (IEL) express an alpha/beta type of T cell receptor (TcR), 13% express a gamma/delta TcR, a percentage which was significantly higher than that found in blood and in lamina propria. In contrast to observations in mice, there was no evidence that normal human TcR gamma/delta+ intestinal IEL might use preferential variable segments of gamma genes. About 10% of human intestinal IEL expressed the alpha chain but not the beta chain of CD8, thus resembling a subset of CD8 alpha+beta- IEL, which was recently described in mice and found to be of thymoindependent origin. In addition, 10% of human IEL had a unique phenotype of immature T cells, as they bore only CD7, but no other T cell or natural killer cell markers. Finally, even the major population of IEL which expressed the usual markers of the T cell lineage (CD3, TcR alpha/beta, CD2, CD4 or CD8 alpha/beta) differed from peripheral blood T lymphocytes by their peculiar expression of surface antigens associated with activation. Indeed, 80% of IEL were CD45R0+, CD45A-, but co-expression of CD11a, CD29 and LFA-3 was inconstant. In addition, 90% of IEL expressed HML-1.

Authors+Show Affiliations

INSERUM U 239, Faculté de Médecine X. Bichat, Hôpital des Enfants Malades, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2141568

Citation

Jarry, A, et al. "Subsets of CD3+ (T Cell Receptor Alpha/beta or Gamma/delta) and CD3- Lymphocytes Isolated From Normal Human Gut Epithelium Display Phenotypical Features Different From Their Counterparts in Peripheral Blood." European Journal of Immunology, vol. 20, no. 5, 1990, pp. 1097-103.
Jarry A, Cerf-Bensussan N, Brousse N, et al. Subsets of CD3+ (T cell receptor alpha/beta or gamma/delta) and CD3- lymphocytes isolated from normal human gut epithelium display phenotypical features different from their counterparts in peripheral blood. Eur J Immunol. 1990;20(5):1097-103.
Jarry, A., Cerf-Bensussan, N., Brousse, N., Selz, F., & Guy-Grand, D. (1990). Subsets of CD3+ (T cell receptor alpha/beta or gamma/delta) and CD3- lymphocytes isolated from normal human gut epithelium display phenotypical features different from their counterparts in peripheral blood. European Journal of Immunology, 20(5), 1097-103.
Jarry A, et al. Subsets of CD3+ (T Cell Receptor Alpha/beta or Gamma/delta) and CD3- Lymphocytes Isolated From Normal Human Gut Epithelium Display Phenotypical Features Different From Their Counterparts in Peripheral Blood. Eur J Immunol. 1990;20(5):1097-103. PubMed PMID: 2141568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subsets of CD3+ (T cell receptor alpha/beta or gamma/delta) and CD3- lymphocytes isolated from normal human gut epithelium display phenotypical features different from their counterparts in peripheral blood. AU - Jarry,A, AU - Cerf-Bensussan,N, AU - Brousse,N, AU - Selz,F, AU - Guy-Grand,D, PY - 1990/5/1/pubmed PY - 1990/5/1/medline PY - 1990/5/1/entrez SP - 1097 EP - 103 JF - European journal of immunology JO - Eur J Immunol VL - 20 IS - 5 N2 - Intestinal intraepithelial lymphocytes (IEL) were studied, after isolation in humans, for their surface antigens with a large variety of monoclonal antibodies. They show peculiar characteristics when compared with peripheral blood lymphocytes and intestinal lamina propria lymphocytes. Although a majority of human intraepithelial lymphocytes (IEL) express an alpha/beta type of T cell receptor (TcR), 13% express a gamma/delta TcR, a percentage which was significantly higher than that found in blood and in lamina propria. In contrast to observations in mice, there was no evidence that normal human TcR gamma/delta+ intestinal IEL might use preferential variable segments of gamma genes. About 10% of human intestinal IEL expressed the alpha chain but not the beta chain of CD8, thus resembling a subset of CD8 alpha+beta- IEL, which was recently described in mice and found to be of thymoindependent origin. In addition, 10% of human IEL had a unique phenotype of immature T cells, as they bore only CD7, but no other T cell or natural killer cell markers. Finally, even the major population of IEL which expressed the usual markers of the T cell lineage (CD3, TcR alpha/beta, CD2, CD4 or CD8 alpha/beta) differed from peripheral blood T lymphocytes by their peculiar expression of surface antigens associated with activation. Indeed, 80% of IEL were CD45R0+, CD45A-, but co-expression of CD11a, CD29 and LFA-3 was inconstant. In addition, 90% of IEL expressed HML-1. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/2141568/Subsets_of_CD3+__T_cell_receptor_alpha/beta_or_gamma/delta__and_CD3__lymphocytes_isolated_from_normal_human_gut_epithelium_display_phenotypical_features_different_from_their_counterparts_in_peripheral_blood_ L2 - https://doi.org/10.1002/eji.1830200523 DB - PRIME DP - Unbound Medicine ER -