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Changes in expression of mRNA for interleukin-8 and effects of interleukin-8 receptor inhibitor in the spinal dorsal horn in a rat model of lumbar disc herniation.
Spine (Phila Pa 1976). 2011 Dec 01; 36(25):2139-46.S

Abstract

STUDY DESIGN

Autologous nucleus pulposus obtained from coccygeal intervertebral discs was grafted on the proximal of L5 dorsal root ganglion. Pain behavior, mRNA expression of Interleukin-8 (IL-8), and immunohistochemical changes were assessed.

OBJECTIVE

The purpose of this study is to investigate temporal changes of IL-8 expression in the spinal cord and dorsal root ganglion and the pain-related behaviors with time course and to elucidate whether repertaxin (IL-8 receptor inhibitor) attenuates pain-related behaviors in a rat model of lumbar disc herniation.

SUMMARY OF BACKGROUND DATA

Inflammatory mediators like cytokines and chemokines have been implicated in radicular pain because of disc herniation. IL-8, known as CXCL8, is a chemokine, which has been reported to be associated with painful degenerative disc disorders and chronic inflammatory pain states.

METHODS

Lumbar disc herniated rat model was made by implantation of the autologous nucleus pulposus, harvested from the coccygeal vertebra of each tail, on the left L5 nerve root just proximal to the dorsal root ganglion. Rats were tested for mechanical allodynia and thermal hyperalgesia at 2 days before surgery, and on days 1, 5, 10, 20, 30, 40, 50, and 60 postoperatively. Experimental group was intrathecally injected with the IL-8 receptor inhibitor at L5 level on postoperative day 10. Mechanical allodynia of the plantar surface of both hindpaw was tested on 30 minutes, 1, 3 hours, 1, 3, 5, and 10 days after administration. For the staining of astrocytes and microglia, immunohistochemical study was done 20 days after surgery.

RESULTS

Mechanical allodynia in ipsilateral hindpaw developed 1 day after surgery and lasted until 60 days and thermal withdrawal latency decreased significantly on the ipsilateral side 10 days after surgery and gradually increased through day 60. The IL-8 receptor inhibitor attenuated the mechanical allodynia caused by nucleus pulposus when it was administered on postoperative day 10 and reduced microglial activation and phosphorylated form of mitogen-activated protein kinase (pERK) expression in the spinal dorsal horn.

CONCLUSION

IL-8 might be a potential therapeutic target in chronic radicular neuropathic pain because of disc herniation, CXCL8 inhibitor could be one of its promising therapeutic agents.

Authors+Show Affiliations

Institute of Medical Science, Yeungnam University, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21415806

Citation

Kim, Su-Jeong, et al. "Changes in Expression of mRNA for Interleukin-8 and Effects of Interleukin-8 Receptor Inhibitor in the Spinal Dorsal Horn in a Rat Model of Lumbar Disc Herniation." Spine, vol. 36, no. 25, 2011, pp. 2139-46.
Kim SJ, Park SM, Cho YW, et al. Changes in expression of mRNA for interleukin-8 and effects of interleukin-8 receptor inhibitor in the spinal dorsal horn in a rat model of lumbar disc herniation. Spine (Phila Pa 1976). 2011;36(25):2139-46.
Kim, S. J., Park, S. M., Cho, Y. W., Jung, Y. J., Lee, D. G., Jang, S. H., Park, H. W., Hwang, S. J., & Ahn, S. H. (2011). Changes in expression of mRNA for interleukin-8 and effects of interleukin-8 receptor inhibitor in the spinal dorsal horn in a rat model of lumbar disc herniation. Spine, 36(25), 2139-46. https://doi.org/10.1097/BRS.0b013e31821945a3
Kim SJ, et al. Changes in Expression of mRNA for Interleukin-8 and Effects of Interleukin-8 Receptor Inhibitor in the Spinal Dorsal Horn in a Rat Model of Lumbar Disc Herniation. Spine (Phila Pa 1976). 2011 Dec 1;36(25):2139-46. PubMed PMID: 21415806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in expression of mRNA for interleukin-8 and effects of interleukin-8 receptor inhibitor in the spinal dorsal horn in a rat model of lumbar disc herniation. AU - Kim,Su-Jeong, AU - Park,Sung-Min, AU - Cho,Yun-Woo, AU - Jung,Yong-Jae, AU - Lee,Dong-Gyu, AU - Jang,Sung-Ho, AU - Park,Hea-Woon, AU - Hwang,Se-Jin, AU - Ahn,Sang-Ho, PY - 2011/3/19/entrez PY - 2011/3/19/pubmed PY - 2012/3/31/medline SP - 2139 EP - 46 JF - Spine JO - Spine (Phila Pa 1976) VL - 36 IS - 25 N2 - STUDY DESIGN: Autologous nucleus pulposus obtained from coccygeal intervertebral discs was grafted on the proximal of L5 dorsal root ganglion. Pain behavior, mRNA expression of Interleukin-8 (IL-8), and immunohistochemical changes were assessed. OBJECTIVE: The purpose of this study is to investigate temporal changes of IL-8 expression in the spinal cord and dorsal root ganglion and the pain-related behaviors with time course and to elucidate whether repertaxin (IL-8 receptor inhibitor) attenuates pain-related behaviors in a rat model of lumbar disc herniation. SUMMARY OF BACKGROUND DATA: Inflammatory mediators like cytokines and chemokines have been implicated in radicular pain because of disc herniation. IL-8, known as CXCL8, is a chemokine, which has been reported to be associated with painful degenerative disc disorders and chronic inflammatory pain states. METHODS: Lumbar disc herniated rat model was made by implantation of the autologous nucleus pulposus, harvested from the coccygeal vertebra of each tail, on the left L5 nerve root just proximal to the dorsal root ganglion. Rats were tested for mechanical allodynia and thermal hyperalgesia at 2 days before surgery, and on days 1, 5, 10, 20, 30, 40, 50, and 60 postoperatively. Experimental group was intrathecally injected with the IL-8 receptor inhibitor at L5 level on postoperative day 10. Mechanical allodynia of the plantar surface of both hindpaw was tested on 30 minutes, 1, 3 hours, 1, 3, 5, and 10 days after administration. For the staining of astrocytes and microglia, immunohistochemical study was done 20 days after surgery. RESULTS: Mechanical allodynia in ipsilateral hindpaw developed 1 day after surgery and lasted until 60 days and thermal withdrawal latency decreased significantly on the ipsilateral side 10 days after surgery and gradually increased through day 60. The IL-8 receptor inhibitor attenuated the mechanical allodynia caused by nucleus pulposus when it was administered on postoperative day 10 and reduced microglial activation and phosphorylated form of mitogen-activated protein kinase (pERK) expression in the spinal dorsal horn. CONCLUSION: IL-8 might be a potential therapeutic target in chronic radicular neuropathic pain because of disc herniation, CXCL8 inhibitor could be one of its promising therapeutic agents. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/21415806/Changes_in_expression_of_mRNA_for_interleukin_8_and_effects_of_interleukin_8_receptor_inhibitor_in_the_spinal_dorsal_horn_in_a_rat_model_of_lumbar_disc_herniation_ L2 - https://doi.org/10.1097/BRS.0b013e31821945a3 DB - PRIME DP - Unbound Medicine ER -