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[Change of Th17 and expression of RORγt in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid].
Zhonghua Yi Xue Za Zhi 2011; 91(6):401-4ZY

Abstract

OBJECTIVE

To study the effects of allergen and corticosteroid on T help 17 (Th17) and orphan nuclear receptor gammat (RORγt) in allergic-rhinitis mice.

METHODS

Experimental allergic rhinitis (AR) was induced by the extract of dermatophagoides pteronyssinus (DP) including 2% ovalbumin (OVA) sensitization in 30 male mice with DP allergen group (n = 10), intranasal corticosteroid (budesonide, BUD) group (n = 10) or without treatment (model group, n = 10). And another 10 were included into the normal control group. The murine levels of Th17 were measured by flow cytometry (FCM). The expression of RORγt mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) while that of RORγt protein in nasal mucosa detected by immunohistochemical staining.

RESULTS

The expression of RORγt in nasal mucosa of AR model was higher than that in the control group (25 ± 5 vs 48 ± 10, P < 0.01). But its expression declined significantly after the administration of local corticosteroid (48 ± 10 vs 31 ± 6, P < 0.01). The levels of RORγt mRNA and Th17 in the AR model group were significantly higher than those in the control group (18.4% ± 1.3% vs 27.5% ± 1.6%, 0.43 ± 0.04 vs 0.64 ± 0.05, both P < 0.01). The levels of RORγt mRNA and Th17 in the spleen of AR mice were significantly reduced by allergen dosing (27.5% ± 1.6% vs 20.0% ± 2.1%, 0.64 ± 0.05 vs 0.54 ± 0.03, both P < 0.01) but not by corticosteroid (P > 0.05).

CONCLUSIONS

Allergen may affect the systemic immunity to inhibit the RORγt expression and block the Th17 differentiation in AR model. Local steroid only produces a marked effect through a down-regulated RORγt expression in nasal mucosa.

Authors+Show Affiliations

Department of Otolaryngology, People's Hospital, Guangxi Zhuang Autonomous Region, Nanning 530021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21418913

Citation

Qu, Shen-Hong, et al. "[Change of Th17 and Expression of RORγt in a Murine Model of Allergic Rhinitis After a Stimulation of Allergen and Corticosteroid]." Zhonghua Yi Xue Za Zhi, vol. 91, no. 6, 2011, pp. 401-4.
Qu SH, Li M, Liang JP, et al. [Change of Th17 and expression of RORγt in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid]. Zhonghua Yi Xue Za Zhi. 2011;91(6):401-4.
Qu, S. H., Li, M., Liang, J. P., Yuan, Y. L., & Lin, Z. B. (2011). [Change of Th17 and expression of RORγt in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid]. Zhonghua Yi Xue Za Zhi, 91(6), pp. 401-4.
Qu SH, et al. [Change of Th17 and Expression of RORγt in a Murine Model of Allergic Rhinitis After a Stimulation of Allergen and Corticosteroid]. Zhonghua Yi Xue Za Zhi. 2011 Feb 15;91(6):401-4. PubMed PMID: 21418913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Change of Th17 and expression of RORγt in a murine model of allergic rhinitis after a stimulation of allergen and corticosteroid]. AU - Qu,Shen-Hong, AU - Li,Min, AU - Liang,Jian-Ping, AU - Yuan,Yu-Lin, AU - Lin,Zhi-Bin, PY - 2011/3/23/entrez PY - 2011/3/23/pubmed PY - 2011/11/16/medline SP - 401 EP - 4 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 91 IS - 6 N2 - OBJECTIVE: To study the effects of allergen and corticosteroid on T help 17 (Th17) and orphan nuclear receptor gammat (RORγt) in allergic-rhinitis mice. METHODS: Experimental allergic rhinitis (AR) was induced by the extract of dermatophagoides pteronyssinus (DP) including 2% ovalbumin (OVA) sensitization in 30 male mice with DP allergen group (n = 10), intranasal corticosteroid (budesonide, BUD) group (n = 10) or without treatment (model group, n = 10). And another 10 were included into the normal control group. The murine levels of Th17 were measured by flow cytometry (FCM). The expression of RORγt mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) while that of RORγt protein in nasal mucosa detected by immunohistochemical staining. RESULTS: The expression of RORγt in nasal mucosa of AR model was higher than that in the control group (25 ± 5 vs 48 ± 10, P < 0.01). But its expression declined significantly after the administration of local corticosteroid (48 ± 10 vs 31 ± 6, P < 0.01). The levels of RORγt mRNA and Th17 in the AR model group were significantly higher than those in the control group (18.4% ± 1.3% vs 27.5% ± 1.6%, 0.43 ± 0.04 vs 0.64 ± 0.05, both P < 0.01). The levels of RORγt mRNA and Th17 in the spleen of AR mice were significantly reduced by allergen dosing (27.5% ± 1.6% vs 20.0% ± 2.1%, 0.64 ± 0.05 vs 0.54 ± 0.03, both P < 0.01) but not by corticosteroid (P > 0.05). CONCLUSIONS: Allergen may affect the systemic immunity to inhibit the RORγt expression and block the Th17 differentiation in AR model. Local steroid only produces a marked effect through a down-regulated RORγt expression in nasal mucosa. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/21418913/[Change_of_Th17_and_expression_of_RORγt_in_a_murine_model_of_allergic_rhinitis_after_a_stimulation_of_allergen_and_corticosteroid]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2011&amp;vol=91&amp;issue=6&amp;fpage=401 DB - PRIME DP - Unbound Medicine ER -