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Development of andrographolide molecularly imprinted polymer for solid-phase extraction.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jun; 79(1):191-6.SA

Abstract

A method employing molecularly imprinted polymer (MIP) as selective sorbent for solid-phase extraction (SPE) to pretreat samples was developed. The polymers were prepared by precipitation polymerization with andrographolide as template molecule. The structure of MIP was characterized and its static adsorption capacity was measured by the Scatchard equation. In comparison with C(18)-SPE and non-imprinted polymer (NIP) SPE column, MIP-SPE column displays high selectivity and good affinity for andrographolide and dehydroandrographolide for extract of herb Andrographis paniculata (Burm.f.) Nees (APN). MIP-SPE column capacity was 11.9±0.6 μmol/g and 12.1±0.5 μmol/g for andrographolide and dehydroandrographolide, respectively and was 2-3 times higher than that of other two columns. The precision and accuracy of the method developed were satisfactory with recoveries between 96.4% and 103.8% (RSD 3.1-4.3%, n=5) and 96.0% and 104.2% (RSD 2.9-3.7%, n=5) for andrographolide and dehydroandrographolide, respectively. Various real samples were employed to confirm the feasibility of method. This developed method demonstrates the potential of molecularly imprinted solid phase extraction for rapid, selective, and effective sample pretreatment.

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Publisher Full Text

Authors+Show Affiliations

Yin X
College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Wanli District, Nanchang, China.
Liu Q
No affiliation info available
Jiang Y
No affiliation info available
Luo Y
No affiliation info available

MeSH

AcrylamideAdsorptionChromatography, High Pressure LiquidDiterpenesLimit of DetectionMolecular ImprintingPolymersRegression AnalysisReproducibility of ResultsSolid Phase ExtractionSpectroscopy, Fourier Transform InfraredTemperature

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21420352

Citation

Yin, Xiaoying, et al. "Development of Andrographolide Molecularly Imprinted Polymer for Solid-phase Extraction." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 79, no. 1, 2011, pp. 191-6.
Yin X, Liu Q, Jiang Y, et al. Development of andrographolide molecularly imprinted polymer for solid-phase extraction. Spectrochim Acta A Mol Biomol Spectrosc. 2011;79(1):191-6.
Yin, X., Liu, Q., Jiang, Y., & Luo, Y. (2011). Development of andrographolide molecularly imprinted polymer for solid-phase extraction. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 79(1), 191-6. https://doi.org/10.1016/j.saa.2011.02.034
Yin X, et al. Development of Andrographolide Molecularly Imprinted Polymer for Solid-phase Extraction. Spectrochim Acta A Mol Biomol Spectrosc. 2011;79(1):191-6. PubMed PMID: 21420352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of andrographolide molecularly imprinted polymer for solid-phase extraction. AU - Yin,Xiaoying, AU - Liu,Qingshan, AU - Jiang,Yifan, AU - Luo,Yongming, Y1 - 2011/02/23/ PY - 2010/12/17/received PY - 2011/02/02/revised PY - 2011/02/16/accepted PY - 2011/3/23/entrez PY - 2011/3/23/pubmed PY - 2011/8/27/medline SP - 191 EP - 6 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 79 IS - 1 N2 - A method employing molecularly imprinted polymer (MIP) as selective sorbent for solid-phase extraction (SPE) to pretreat samples was developed. The polymers were prepared by precipitation polymerization with andrographolide as template molecule. The structure of MIP was characterized and its static adsorption capacity was measured by the Scatchard equation. In comparison with C(18)-SPE and non-imprinted polymer (NIP) SPE column, MIP-SPE column displays high selectivity and good affinity for andrographolide and dehydroandrographolide for extract of herb Andrographis paniculata (Burm.f.) Nees (APN). MIP-SPE column capacity was 11.9±0.6 μmol/g and 12.1±0.5 μmol/g for andrographolide and dehydroandrographolide, respectively and was 2-3 times higher than that of other two columns. The precision and accuracy of the method developed were satisfactory with recoveries between 96.4% and 103.8% (RSD 3.1-4.3%, n=5) and 96.0% and 104.2% (RSD 2.9-3.7%, n=5) for andrographolide and dehydroandrographolide, respectively. Various real samples were employed to confirm the feasibility of method. This developed method demonstrates the potential of molecularly imprinted solid phase extraction for rapid, selective, and effective sample pretreatment. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/21420352/Development_of_andrographolide_molecularly_imprinted_polymer_for_solid_phase_extraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(11)00127-2 DB - PRIME DP - Unbound Medicine ER -